Site-directed mutagenesis of multi-copy-number plasmids: Red/ET recombination and unique restriction site elimination

BioTechniques

Volumn 46, Issue 7, 2009, Pages 527-533

  Noll, Stephan ^a   Hampp, Gabriele ^a   Bausbacher, Hanna ^a   Pellegata, Natalia S ^b   Kranz, Harald ^a  

^a Gene Bridges GmbH   (Germany)

^b INSTITUTE OF EPIDEMIOLOGY   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

DNA FRAGMENT; NUCLEOTIDE; OLIGONUCLEOTIDE; PRIMER DNA;

AMPLICON; ANIMAL CELL; ARTICLE; BACTERIAL CELL; CODON; DIGESTION; DNA SEQUENCE; DNA SYNTHESIS; ESCHERICHIA COLI; GENE AMPLIFICATION; GENE CASSETTE; GENE DELETION; GENE TARGETING; GENE VECTOR; IN VITRO STUDY; MOUSE; MUTAGENESIS; NONHUMAN; PLASMID; RECOMBINANT PLASMID; RESTRICTION SITE; SITE DIRECTED MUTAGENESIS; SPECTRUM; ANIMAL; EXON; GENE DOSAGE; GENETIC RECOMBINATION; GENETIC TRANSFORMATION; GENETICS; METABOLISM; METHODOLOGY; MOLECULAR GENETICS; NUCLEOTIDE SEQUENCE; POINT MUTATION; RESTRICTION MAPPING;

ESCHERICHIA COLI;

ANIMALS; BASE SEQUENCE; CODON; EXONS; GENE DOSAGE; GENETIC VECTORS; MICE; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; OLIGONUCLEOTIDES; PLASMIDS; POINT MUTATION; RECOMBINATION, GENETIC; RESTRICTION MAPPING; TRANSFORMATION, GENETIC;

EID: 70349258338     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/000113150     Document Type: Article

References (25)

1. Smith, M. 1985. In vitro mutagenesis. Annu. Rev. Genet. 19:423-462.
2. Higuchi, R., B. Krummel, and R.K. Saiki. 1988. A general method of in vitro preparation and specific mutagenesis of DNA fragments: study of protein and DNA interactions. Nucleic Acids Res. 16:7351-7367.
3. Shimada, A. 1996. PCR-based site-directed mutagenesis. Methods Mol. Biol. 57:157-165.
4. Ho, S.N., H.D. Hunt, R.M. Horton, J.K. Pullen, and L.R. Pease. 1989. Site-directed mutagenesis by overlap extension using the polymerase chain reaction. Gene 77:51-59. (Pubitemid 19125653)
5. Horton, R.M., H.D. Hunt, S.N. Ho, J.K. Pullen, and L.R. Pease. 1989. Engineering hybrid genes without the use of restriction enzymes: gene splicing by overlap extension. Gene 77:61-68.
6. Sarkar, G. and S.S. Sommer. 1990. The "megaprimer" method of site-directed mutagenesis. BioTechniques 8:404-407.
7. Zoller, M.J. and M. Smith. 1982. Oligo-nucleotide- directed mutagenesis using M13-derived vectors: an efficient and general procedure for the production of point mutations in any fragment of DNA. Nucleic Acids Res. 10:6487-6500.
8. Deng, W.P. and J.A. Nickoloff. 1992. Site-directed mutagenesis of virtually any plasmid by eliminating a unique site. Anal. Biochem. 200:81-88.
9. Sawitzke, J.A., L.C. Thomason, N. Costantino, M. Bubunenko, S. Datta, and D.L. Court. 2007. Recombineering: in vivo genetic engineering in E. coli, S. enterica, and beyond. Methods Enzymol. 421:171-199.
10. Zhang, Y., J.P. Muyrers, G. Testa, and A.F. Stewart. 2000. DNA cloning by homologous recombination in Escherichia coli. Nat. Biotechnol. 18:1314-1317.
11. Warming, S., N. Costantino, D.L. Court, N.A. Jenkins, and N.G. Copeland. 2005. Simple and highly efficient BAC recombineering using galK selection. Nucleic Acids Res. 33:e36.
12. Wong, Q.N., V.C. Ng, M.C. Lin, H.F. Kung, D. Chan, and J.D. Huang. 2005. Efficient and seamless DNA recombineering using a thymidylate synthase A selection system in Escherichia coli. Nucleic Acids Res. 33:e59.
13. DeVito, J.A. 2008. Recombineering with tolC as a selectable/counter- selectable marker: remodeling the rRNA operons of Escherichia coli. Nucleic Acids Res. 36:e4.
14. Heermann, R., T. Zeppenfeld, and K. Jung. 2008. Simple generation of site-directed point mutations in the Escherichia coli chromosome using Red/ET recombination. Microb. Cell Fact. 7:14.
15. Thomason, L.C., N. Costantino, D.V. Shaw, and D.L. Court. 2007. Multicopy plasmid modification with phage lambda Red recombineering. Plasmid 58:148-158.
16. Zhang, Y., F. Buchholz, J.P. Muyrers, and A.F. Stewart. 1998. A new logic for DNA engineering using recombination in Escherichia coli. Nat. Genet. 20:123-128.
17. Yu, D., H.M. Ellis, E.C. Lee, N.A. Jenkins, N.G. Copeland, and D.L. Court. 2000. An efficient recombination system for chromosome engineering in Escherichia coli. Proc. Natl. Acad. Sci. USA 97:5978-5983.
18. Dower, W.J., J.F. Miller, and C.W. Ragsdale. 1988. High efficiency transformation of E. coli by high voltage electroporation. Nucleic Acids Res. 16:6127-6145.
19. Sambrook, J. and D.W. Russell. 2001. Molecular Cloning: A Laboratory Manual, 3rd ed. CSH Laboratory Press, Cold Spring Harbor, NY.
20. Inselburg, J. 1974. Replication of colicin E1 plasmid DNA in minicells from a unique replication initiation site. Proc. Natl. Acad. Sci. USA 71:2256-2259.
21. Shigekawa, K. and W.J. Dower. 1988. Electroporation of eukaryotes and prokaryotes: a general approach to the introduction of macromolecules into cells. BioTechniques 6:742-751.
22. Thomason, L., D.L. Court, M. Bubunenko, N. Costantino, H. Wilson, S. Datta, and A. Oppenheim. 2007. Recombineering: genetic engineering in bacteria using homologous recombination. In Current Protocols in Molecular Biology, John Wiley and Sons, New York.
23. Kramer, B., W. Kramer, and H.J. Fritz. 1984. Different base/base mismatches are corrected with different efficiencies by the methyl-directed DNA mismatch-repair system of E. coli. Cell 38:879-887.
24. Zell, R. and H.J. Fritz. 1987. DNA mismatch-repair in Escherichia coli counteracting the hydrolytic deamination of 5-methyl-cytosine residues. EMBO J. 6:1809-1815.
25. Schofield, M.J. and P. Hsieh. 2003. DNA mismatch repair: molecular mechanisms and biological function. Annu. Rev. Microbiol. 57:579-608.