Generation of chimeras by morula aggregation

Methods in Molecular Biology

Volumn 561, Issue , 2009, Pages 219-229

  Plück, Anne ^a   Klasen, Christian ^a  

^a UNIVERSITY OF COLOGNE   (Germany)

Author keywords

Electrofusion; Embryo donor mice; ES cell transfer techniques; Gene targeting; Germline transmission; Morula aggregation; Preimplantation stage embryos; Sandwich aggregation; Stud males; Tetraploid embryos

Indexed keywords

MUS;

ANIMAL; ANIMAL EMBRYO; ARTICLE; BLASTOCYST; CELL AGGREGATION; CELL DIFFERENTIATION; CHIMERA; CYTOLOGY; EMBRYO CULTURE; EMBRYONIC STEM CELL; FETAL WELL BEING; GENETICS; METHODOLOGY; MICROINJECTION; MORULA; MOUSE; PHYSIOLOGY; PLOIDY; POLYMERASE CHAIN REACTION; TRANSGENIC ANIMAL;

ANIMALS; ANIMALS, GENETICALLY MODIFIED; BLASTOCYST; CELL AGGREGATION; CELL DIFFERENTIATION; CHIMERA; EMBRYO CULTURE TECHNIQUES; EMBRYO, MAMMALIAN; EMBRYONIC STEM CELLS; FETAL VIABILITY; MICE; MICROINJECTIONS; MORULA; PLOIDIES; POLYMERASE CHAIN REACTION;

EID: 70349234213     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-60327-019-9_14     Document Type: Article

References (4)

1. Tarkowsky, A. K. (1961) Mouse chimaeras from fused eggs. Nature 190, 857-860.
2. Thomas, K. R., and Capecchi M. R. (1990) Targeted disruption of the murine int-1 protooncogene resulting in severe abnormalities in midbrain and cerebellar development. Nature 346, 847-850.
3. Wood, S. A., Allen, N. D., Rossant, J., Auerbach, A., and Nagy, A. (1993) Noninjection methods for the production of chimeric stem cell-embryo chimeras. Nature 365, 87-89.
4. Wang, Z., and Jaenisch, R. (2004) At most three ES cells contribute to the somatic lineages of chimeric mice and of mice produced by ES-tetraploid complementation. Dev. Biol 275, 192-201.