Integrated microscopy for real-time imaging of mechanotransduction studies in live cells

Journal of Biomedical Optics

Volumn 14, Issue 3, 2009, Pages

  Trache, Andreea ^a,b   Lim, Soon Mi ^a,b  

^a TEXAS A M COLLEGE OF MEDICINE   (United States)

^b TEXAS A AND M UNIVERSITY   (United States)

Author keywords

atomic force microscopy; integrated microscopy; spinning disk confocal; total internal reflection fluorescence

Indexed keywords

3D IMAGING; ADHESION SITES; AFM; ATOMIC FORCE MICROSCOPES; CELL ATTACHMENTS; CELL RESPONSE; CELLULAR BEHAVIORS; FOCAL ADHESIONS; HIGH PRECISION; HIGH-CONTRAST IMAGES; INTEGRATED MICROSCOPY; INTEGRATED SYSTEMS; INTRACELLULAR SIGNALING; LIVE CELL; LIVING CELL; MECHANICAL FORCE; MECHANICAL STIMULATION; MECHANOTRANSDUCTION; OPTICAL IMAGING; OPTICAL IMAGING TECHNIQUE; REAL TIME; REALTIME IMAGING; SPINNING-DISK CONFOCAL; SUB-CELLULAR; TEMPORAL RESOLUTION; TIRF MICROSCOPY; TOTAL INTERNAL REFLECTION FLUORESCENCE; TRANSMISSION OF FORCES; VASCULAR SMOOTH MUSCLE CELLS;

ADHESION; CELL MEMBRANES; CONFOCAL MICROSCOPY; CYTOLOGY; DYNAMICS; FLUORESCENCE; IMAGING TECHNIQUES; MECHANICAL ENGINEERING; MOLECULAR DYNAMICS; OPTICAL IMAGE STORAGE; REFRACTIVE INDEX; SIGNAL TRANSDUCTION; SURFACE TOPOGRAPHY;

ATOMIC FORCE MICROSCOPY;

ANIMAL; ARTICLE; ATOMIC FORCE MICROSCOPY; CELL; CELL FUNCTION; COMPUTER PROGRAM; CONFOCAL MICROSCOPY; CULTURE TECHNIQUE; CYTOLOGY; CYTOSKELETON; EPITHELIUM CELL; EQUIPMENT DESIGN; FLUORESCENCE MICROSCOPY; INSTRUMENTATION; LASER; LIVER; MECHANOTRANSDUCTION; METHODOLOGY; MICROSCOPY; PHYSIOLOGY; RAT; SYSTEM ANALYSIS; TIME;

ANIMALS; CELL CULTURE TECHNIQUES; CELL PHYSIOLOGICAL PHENOMENA; CELLS; CYTOSKELETON; EPITHELIAL CELLS; EQUIPMENT DESIGN; LASERS; LIVER; MECHANOTRANSDUCTION, CELLULAR; MICROSCOPY; MICROSCOPY, ATOMIC FORCE; MICROSCOPY, CONFOCAL; MICROSCOPY, FLUORESCENCE; MICROSCOPY, VIDEO; RATS; SOFTWARE; SYSTEMS INTEGRATION; TIME FACTORS;

EID: 70349231029     PISSN: 10833668     EISSN: 15602281     Source Type: Journal    
DOI: 10.1117/1.3155517     Document Type: Article

References (48)

1. A., Trache and G. A., Meininger, " Atomic force - multi optical imaging integrated microscope for monitoring molecular dynamics in live cells, ", J. Biomed. Opt., 10 (6), 064023 (2005). jbo JBOPFO 1083-3668 10.1117/1.2146963
2. G., Binnig, C. F., Quate, and C. H., Gerber, " Atomic force microscope, ", Phys. Rev. Lett., 56 (9), 930-933 (1986). prl PRLTAO 0031-9007 10.1103/PhysRevLett.56.930
3. S., Inoue and T., Inoue, " Direct-view high-speed confocal scanner: the CSU-10, ", Methods Cell Biol., 70, 87-127 (2002). 637 MCBLAG 0091-679X 10.1016/S0091-679X(02)70003-4
4. D., Axelrod, " Selective imaging of surface fluorescence with very high aperture microscope objectives, ", J. Biomed. Opt., 6 (6), 6-13 (2001). jbo JBOPFO 1083-3668 10.1117/1.1335689
5. D. E., Ingber, L., Dike, L., Hansen, S., Karp, H., Liley, A., Maniotis, H., McNamee, D., Mooney, G., Plopper, J., Sims, and N., Wang, " Cellular tensegrity: exploring how mechanical changes in the cytoskeleton regulate cell growth, migration, and tissue pattern during morphogenesis, ", Int. Rev. Cytol., 150, 173-224 (1994). iry IRCYAJ 0074-7696 10.1016/S0074-7696(08)61542-9
6. Y., Sawada and M. P., Sheetz, " Force transduction by Triton cytoskeletons, ", J. Cell Biol., 156 (4), 609-615 (2002). jla JCLBA3 0021-9525 10.1083/jcb.200110068
7. J. R., Sims, S., Karp, and D. E., Ingber, " Altering the cellular mechanical force balance results in integrated changes in cell, cytoskeletal and nuclear shape, ", J. Cell. Sci., 103 (4), 1215-1222 (1992). jcb JNCSAI 0021-9533
8. N. F., Worth, B. E., Rolfe, J., Song, and G. R., Campbell, " Vascular smooth muscle cell phenotypic modulation in culture is associated with reorganization of contractile and cytoskeletal proteins, ", Cell Motil. Cytoskeleton, 49 (3), 130-145 (2001). cms CMCYEO 0886-1544 10.1002/cm.1027
9. J. D., Humphries, P., Wang, C., Streuli, B., Geiger, M. J., Humphries, and C., Ballestrem, " Vinculin controls focal adhesion formation by direct interactions with talin and actin, ", J. Cell Biol., 179 (5), 1043-1057 (2007). jla JCLBA3 0021-9525 10.1083/jcb.200703036
10. Y. L., Hu, J. H., Haga, H., Miao, Y., Wang, Y. S., Li, and S., Chien, " Roles of microfilaments and microtubules in paxillin dynamics, ", Biochem. Biophys. Res. Commun., 348 (4), 1463-1471 (2006). bbr BBRCA9 0006-291X 10.1016/j.bbrc.2006.08.022
11. J., Pourati, A., Maniotis, D., Spiegel, J. L., Schaffer, J. P., Butler, J. J., Fredberg, D. E., Ingber, D., Stamenovic, and N., Wang, " Is cytoskeletal tension a major determinant of cell deformability in adherent endothelial cells ?", Am. J. Physiol.: Cell Physiol., 274 (5), C1283-C1289 (1998). bde AJPHAP 0363-6143
12. P. A., Janmey, " The cytoskeleton and cell signaling: component localization and mechanical coupling, ", Physiol. Rev., 78 (3), 763-775 (1998). psi PHREA7 0031-9333
13. A. O., Saez, W., Zhang, Y., Wu, C. E., Turner, D. D., Tang, and S. J., Gunst, " Tension development during contractile stimulation of smooth muscle requires recruitment of paxilin and vinculin to the membrane, ", Am. J. Physiol.: Cell Physiol., 286 (2), C433-C447 (2004). bde AJPHAP 0363-6143 10.1152/ajpcell.00030.2003
14. F. J., Alenghat and D. E., Ingber, " Mechanotransduction: all signals point to cytoskeleton, matrix and integrins, ", Sci. STKE, 2002 (119), 1-4 (2002). d7z ZZZZZZ 1525-8882 10.1126/stke.2002.119.pe6
15. E., Zamir and B., Geiger, " Molecular complexity and dynamics of cell-matrix adhesions, ", J. Cell. Sci., 114 (20), 3583-3590 (2001). jcb JNCSAI 0021-9533
16. T. P., Lele, C. K., Thodeti, and D. E., Ingber, " Force meets chemistry: analysis of mechanochemical conversion in focal adhesions using fluorescence recovery after photobleaching, ", J. Cell. Biochem., 97 (6), 1175-1183 (2006). cbc JCEBD5 0730-2312 10.1002/jcb.20761
17. B., Geiger and A., Bershdsky, " Assembly and mechanosensory function of focal contacts, ", Curr. Opin. Cell Biol., 13 (5), 584-592 (2001). coc COCBE3 0955-0674 10.1016/S0955-0674(00)00255-6
18. H. R., Romer, K. G., Birukov, and J. G. N., Garcia, " Focal adhesions. Paradigm for a signaling nexus, ", Circ. Res., 98 (5), 606-616 (2006). cis CIRUAL 0009-7330 10.1161/01.RES.0000207408.31270.db
19. S. K., Sastry and K., Burridge, " Focal adhesions: a nexus for intracellular signaling and cytoskeletal dynamics, ", Exp. Cell Res., 261 (1), 25-36 (2000). ecr ECREAL 0014-4827 10.1006/excr.2000.5043
20. K., Burridge and M., Chrzanowska-Wodnicka, " Focal adhesions, contractility and signaling, ", Annu. Rev. Cell Dev. Biol., 12, 463-518 (1996). arw ARDBF8 1081-0706 10.1146/annurev.cellbio.12.1.463
21. R., Zaidel-Bar, R., Milo, Z., Kam, and B., Geiger, " A paxillin tyrosine phosphorylation switch regulates the assembly and form of cell-matrix adhesions, ", J. Cell. Sci., 120 (Pt 1), 137-48 (2007). jcb JNCSAI 0021-9533 10.1242/jcs.03314
22. D., Tang, D., Mehta, and S. J., Gunst, " Mechanosensitive tyrosine phosphorylation of paxillin and focal adhesion kinase in tracheal smooth muscle, ", Am. J. Physiol.: Cell Physiol., 276 (1), C250-258 (1999). bde AJPHAP 0363-6143
23. C. E., Turner, " Paxillin and focal adhesion signaling, ", Nat. Cell Biol., 2 (12), E231-E236 (2000). 9go NCBIFN 1465-7392 10.1038/35046659
24. M., Yoshigi, L. M., Hoffman, C. C., Jensen, H. J., Yost, and M. C., Beckerle, " Mechanical force mobilizes zyxin from focal adhesions to actin filaments and regulates cytoskeletal reinforcement, ", J. Cell Biol., 171 (2), 209-215 (2005). jla JCLBA3 0021-9525 10.1083/jcb.200505018
25. M., Cattaruza, C., Lattrich, and M., Hecker, " Focal adhesion protein Zyxin is a mechanosensitive modulator of gene expression in vascular smooth muscle cells, ", Hypertension, 43 (4), 726-730 (2004). hyt HPRTDN 0194-911X 10.1161/01.HYP.0000119189.82659.52
26. Y., Wang and T. D., Gilmore, " Zyxin and paxillin proteins: focal adhesion plaque LIM domain proteins go nuclear, ", Biochim. Biophys. Acta, 1593 (2-3), 115-120 (2003). bba BBACAQ 0006-3002 10.1016/S0167-4889(02)00349-X
27. R. Y., Tsien, " Imagining imaging's future, ", Nat. Rev. Mol. Cell Biol., 4, Suppl. SS16-21 (2003). b9r ZZZZZZ 1471-0072
28. D., Axelrod and G. M., Omann, " Combinatorial microscopy, ", Nat. Rev. Mol. Cell Biol., 7 (12), 944-952 (2006). b9r ZZZZZZ 1471-0072 10.1038/nrm2062
29. S. R., Heidemann and D., Wirtz, " Towards a regional approach to cell mechanics, ", Trends Cell Biol., 14 (4), 160-166 (2004). tcb TCBIEK 0962-8924 10.1016/j.tcb.2004.02.003
30. K. J., Van Vliet, G., Bao, and S., Suresh, " The biomechanics toolbox: experimental approaches for living cells and biomolecules, ", Acta Mater., 51 (19), 5881-5905 (2003). acz ACMAFD 1359-6454 10.1016/j.actamat.2003. 09.001
31. M. C., Adams, W. C., Salmon, S. L., Gupton, C. S., Cohan, T., Wittmann, N., Prigozhina, and C. M., Waterman-Storer, " A high-speed multispectral spinning-disk confocal microscope system for fluorescent speckle microscopy of living cells, ", Methods, 29 (1), 29-41 (2003). 8as ZZZZZZ 1046-2023 10.1016/S1046-2023(02)00282-7
32. R. J., Graf, J., Rietdorf, and T., Zimmermann, " Live cell spinning disk microscopy, ", Adv. Biochem. Eng./Biotechnol., 95, 57-75 (2005). 28w ABEBDZ 0724-6145
33. E., Wang, C. M., Babbey, and K. W., Dunn, " Performance comparison between the high-speed Yokogawa spinning disc confocal system and single-point scanning confocal systems, ", J. Microsc., 218 (Pt 2), 148-159 (2005). jmi JMICAR 0022-2720 10.1111/j.1365-2818.2005.01473.x
34. D., Axelrod, N. L., Thompson, and T. P., Burghardt, " Total internal reflection fluorescent microscopy, ", J. Microsc., 129, 19-28 (1983). jmi JMICAR 0022-2720
35. D., Axelrod, T. P., Burghardt, and N. L., Thompson, " Total internal reflection fluorescence, ", Annu. Rev. Biophys. Bioeng., 13, 247-268 (1984). abp ABPBBK 0084-6589 10.1146/annurev.bb.13.060184.001335
36. A., Stout and D., Axelrod, " Evanescent field excitation of fluorescence by epi-illumination microscopy, ", Appl. Opt., 28 (24), 5237-5242 (1989). apo APOPAI 0003-6935 10.1364/AO.28.005237
37. W. M., Reichert, " Evanscent detection of absorbed films: assessment of optical considerations for absorbance and fluorescence spectroscopy at the crystal/solution and polymer/solution interfaces, ", Crit. Rev. Biocompat., 5, 173-205 (1989).
38. W. M., Reichert and G. A., Truskey, " Total internal reflection fluorescence (TIRF) microscopy. I. Modeling cell contact region fluorescence, ", J. Cell. Sci., 96 (2), 219-230 (1990). jcb JNCSAI 0021-9533
39. J. S., Burmeister, L. A., Olivier, G. A., Truskey, and W. M., Reichert, " Application of total internal reflection fluorescence microscopy to study cell adhesion to biomaterials, ", Biomaterials, 19 (4-5), 307-325 (1998). bit BIMADU 0142-9612 10.1016/S0142-9612(97)00109-9
40. M., Radmacher, R. W., Tillman, M., Fritz, and H. E., Gaub, " From molecules to cells: imaging soft samples with the atomic force microscope, ", Science, 257 (5078), 1900-1905 (1992). sci SCIEAS 0036-8075 10.1126/science.1411505
41. M., Gad and A., Ikai, " Imaging living cells and mapping their surface molecules with the atomic force microscope, " in, Methods in Cellular Imaging, A., Periasamy, Ed., pp. 409-423 (2001).
42. A., Trache, J. P., Trzeciakowski, L., Gardiner, Z., Sun, M., Muthuchamy, M., Guo, S. Y., Yuan, and G. A., Meininger, " Histamine effects on endothelial cell fibronectin interaction studied by atomic force microscopy, ", Biophys. J., 89 (4), 2888-2898 (2005). bio BIOJAU 0006-3495 10.1529/biophysj.104.057026
43. H. J., Butt, B., Cappella, and M., Kappl, " Force measurements with atomic force microscope: technique, interpretation and applications, ", Surf. Sci. Rep., 59 (1-6), 1-152 (2005). ssr SSREDI 0167-5729 10.1016/j.surfrep.2005.08.003
44. O. H., Willemsen, M. M., Snel, A., Cambi, J., Greve, B. G., De Grooth, and C. G., Figdor, " Biomolecular interactions measured by atomic force microscopy, ", Biophys. J., 79 (6), 3267-3281 (2000). bio BIOJAU 0006-3495 10.1016/S0006-3495(00)76559-3
45. E. P., Wojcikiewicz, X., Zhang, and V. T., Moy, " Force and compliance measurements on living cells using atomic force microscopy (AFM), ", Bio. Proceed Online, 6, 1-9 (2004). 10.1251/bpo67
46. F., Chong, C. G., Coates, D. J., Denvir, N., McHale, K., Thornbury, and M., Hollywood, " Optimization of spinning disk confocal microscopy: synchronization with the ultra-sensitive EMCCD, ", Proc. SPIE, 5324, 65-76 (2004). spi PSISDG 0277-786X 10.1117/12.529220
47. X., Wu, G. E., Davis, G. A., Meininger, E., Wilson, and M. J., Davis, " Regulation of the L-type calcium channel by alpha 5-beta 1 integrin requires signaling between focal adhesion proteins, ", J. Biol. Chem., 276 (32), 30285-30292 (2001). jbc JBCHA3 0021-9258 10.1074/jbc.M102436200
48. R., Barhoumi, Y., Mouneimne, I., Awooda, S. H., Safe, K. C., Donnelly, and R. C., Burghardt, " Characterization of calcium oscillations in normal and benzo[a]pyrene-treated Clone 9 cells, ", Toxicol. Sci., 68 (2), 444-450 (2002). 8ir TOSCF2 1096-6080 10.1093/toxsci/68.2.444