Characterization of single heat-activated Bacillus spores using laser tweezers a Raman spectroscopy

Optics Express

Volumn 17, Issue 19, 2009, Pages 16480-16491

  Zhang, Pengfei ^a   Setlow, Peter ^b   Li, Yongqing ^a  

^a EAST CAROLINA UNIVERSITY   (United States)

^b UNIVERSITY OF CONNECTICUT HEALTH CENTER   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BACILLUS CEREUS; BACTERIOLOGY; CHEMICAL ACTIVATION; PRINCIPAL COMPONENT ANALYSIS; PROTEINS; RAMAN SCATTERING; RAMAN SPECTROSCOPY;

LASER TWEEZERS RAMAN SPECTROSCOPIES; PARTIAL DENATURATION; POLARIZED COMPONENTS; POLARIZED RAMAN SPECTRA; PRINCIPLE COMPONENT ANALYSIS; PROTEIN DENATURATION; SPECTRAL DIFFERENCES; SUBLETHAL TEMPERATURES;

ACTIVATION ANALYSIS;

ARTICLE; BACILLUS CEREUS; BACILLUS SUBTILIS; BACTERIAL SPORE; HEAT; METHODOLOGY; OPTICAL TWEEZERS; PHYSIOLOGY; PRINCIPAL COMPONENT ANALYSIS; RAMAN SPECTROMETRY;

BACILLUS CEREUS; BACILLUS SUBTILIS; HOT TEMPERATURE; OPTICAL TWEEZERS; PRINCIPAL COMPONENT ANALYSIS; SPECTRUM ANALYSIS, RAMAN; SPORES, BACTERIAL;

EID: 70349195018     PISSN: None     EISSN: 10944087     Source Type: Journal    
DOI: 10.1364/OE.17.016480     Document Type: Article

References (34)

1. P. Setlow, "Spores of Bacillus subtilis: their resistance to and killing by radiation, heat and chemicals," J. Appl. Microbiol. 101(3), 514-525 (2006).
2. P. Setlow, "Spore germination," Curr. Opin. Microbiol. 6(6), 550-556 (2003).
3. F. R. Evans, and H. R. Curran, "The accelerating effect of sublethal heat on spore germination in mesophilic aerobic bacteria," J. Bacteriol. 46(6), 513-523 (1943).
4. A. Keynan, and Z. Evenchik, "Activation", in The Bacterial Spore, G.W. Gould and A. Hurst, eds. (Academic, 1969), pp. 359-396.
5. A. M. Cook, and M. R. W. Brown, "Relationship between heat activation and percentage colony formation for Bacillus stearothermophilus spores: effects of storage and pH of the recovery medium," J. Appl. Bacteriol. 28(3), 361-364 (1965).
6. J. H. Martin, and P. W. Blackwood, "Effects of sub-lethal heat-shock, L-alanine, and L-alanine on germination and subsequent destruction of Bacillus spores by pasteurization," J. Dairy Sci. 55, 577-580 (1971).
7. S. J. Foster, and K. Johnstone, "The trigger mechanism of bacterial spore germination", in Regulation of Prokaryotic Development, I. Smith, R.A. Slepecky, P. Setlow, eds. (American Society for Microbiology, 1989), pp. 89-108.
8. S. Ablett, A. H. Darke, P. J. Lillford, and D. R. Martin, "Glass formation and dormancy in bacterial spores," Int. J. Food Sci. Technol. 34(1), 59-69 (1999).
9. R. G. K. Leuschner, and P. J. Lillford, "Thermal properties of bacterial spores and biopolymers," Int. J. Food Microbiol. 80(2), 131-143 (2003).
10. V. Sapru, and T. P. Labuza, "Glassy state in bacterial spores predicted by polymer glass-transition theory," J. Food Sci. 58(2), 445-448 (1993).
11. G. W. Gould, and A. D. Hitchins, "Sensitization of bacterial spores to lysozyme and to hydrogen peroxide with agents which rupture disulfide bonds," J. Gen. Microbiol. 33, 413-1123 (1962).
12. A. Alimova, A. Katz, P. Gottlieb, and R. R. Alfano, "Proteins and dipicolinic acid released during heat shock activation of Bacillus subtilis spores probed by optical spectroscopy," Appl. Opt. 45(3), 445-450 (2006).
13. W. E. Huang, R. I. Griffiths, I. P. Thompson, M. J. Bailey, and A. S. Whiteley, "Raman microscopic analysis of single microbial cells," Anal. Chem. 76(15), 4452-4458 (2004).
14. A. Mahadevan-Jansen, and R. Richards-Kortum, "Raman spectroscopy for the detection of cancers and precancers," J. Biomed. Opt. 1(1), 31-70 (1996).
15. C G. Xie, M. A. Dinno, and Y. Q. Li, "Near-infrared Raman spectroscopy of single optically trapped biological cells," Opt. Lett. 27(4), 249-251 (2002).
16. 2+-dipicolinic acid in individual Bacillus spores determined using microfluidic Raman tweezers," J. Bacteriol. 189(13), 4681-4687 (2007).
17. C Creely, G. Singh, and D. Petrov, "Dual wavelength optical tweezers for confocal Raman spectroscopy," Opt. Commun. 245(1-6), 465-470 (2005).
18. A. C De Luca, G. Rusciano, R. Ciancia, V. Martinelli, G. Pesce, B. Rotoli, L. Selvaggi, and A. Sasso, "Spectroscopical and Mechanical Characterization of Normal and Thalassemic Red Blood Cells by Raman Tweezers," Opt. Express 16(11), 7943-7957 (2008).
19. D. Chen, S. S. Huang, and Y. Q. Li, "Real-time detection of kinetic germination and heterogeneity of single Bacillus spores by laser tweezers Raman spectroscopy," Anal. Chem. 78(19), 6936-6941 (2006).
20. L. Peng, D. Chen, P. Setlow, and Y. Q. Li, "Elastic and inelastic light scattering from single bacterial spores in an optical trap allows the monitoring of spore germination dynamics," Anal. Chem. 81(10), 4035-4042 (2009).
21. C Xie, D. Chen, and Y. Q. Li, "Raman sorting and identification of single living micro-organisms with optical tweezers," Opt. Lett. 30(14), 1800-1802 (2005).
22. C Xie, J. Mace, M. A. Dinno, Y. Q. Li, W. Tang, R. J. Newton, and P. J. Gemperline, "Identification of single bacterial cells in aqueous solution using confocal laser tweezers Raman spectroscopy," Anal. Chem. 77(14), 4390-4397 (2005).
23. D. Chen, L. Shelenkova, Y. Q. Li, C R. Kempf, and A. Sabelnikov, "Laser tweezers Raman spectroscopy potential for studies of complex dynamic cellular processes: single cell bacterial lysis," Anal. Chem. 81(9), 3227-3238 (2009).
24. J. R. Ferraro, K. Nakamoto, and C. W. Brown, Introductory Raman spectroscopy, 2nd Edition. (Academic, 2000), pp. 267-293.
25. A. C.-T. Ko, L.-P. Choo-Smith, M. Hewko, M. G. Sowa, C C Dong, and B. Cleghorn, "Detection of early dental caries using polarized Raman spectroscopy," Opt. Express 14(1), 203-215 (2006).
26. H. Liem, J. Cabanillas-Gonzalez, P. Etchegoin, and D. D. C Bradley, "Glass transition temperatures of polymer thin films monitored by Raman scattering," J. Phys. Condens. Matter 16(6), 721-728 (2004).
27. J. M. Benevides, M. Tsuboi, J. K. H. Bamford, and G. J. Thomas, Jr., "Polarized Raman spectroscopy of doublestranded RNA from bacteriophage 46: local Raman tensors of base and backbone vibrations," Biophys. J. 72(6), 2748-2762 (1997).
28. S. Ghosh, and P. Setlow, "The preparation, germination properties and stability of superdormant spores of Bacillus cereus," J. Appl. Microbiol, (to be published).
29. M. Paidhungat, B. Setlow, A. Driks, and P. Setlow, "Characterization of spores of Bacillus subtilis which lack dipicolinic acid," J. Bacteriol. 182(19), 5505-5512 (2000).
30. W. L. Nicholson, and P. Setlow, "Sporulation, germination and outgrowth", in Molecular Biological Methods for Bacillus, C. R. Harwood, and S. M. Cutting, eds. (John Wiley, 1990), pp. 391-450.
31. T. Kitagawa, and S. Hirota, "Raman spectroscopy of proteins", in Handbook of Vibrational Spectroscopy, volume 5, J. M. Chalmers and P. R. Griffiths, eds. (John Wiley, 2002), pp. 3426-3446.
32. V. J. C Lin, and J. L. Koenig, "Raman studies of bovine serum albumin", Biopoly. 15(1), 203-218 (1976).
33. W. H. Coleman, D. Chen, Y. Q. Li, A. E. Cowan, and P. Setlow, "How moist heat kills spores of Bacillus subtilis," J. Bacteriol. 189(23), 8458-8466 (2007).
34. S. Ghosh, P. Zhang, Y.-q. Li, and P. Setlow, "Superdormant spores of Bacillus species have elevated wet heat resistance and temperature requirements for heat activation", (unpublished).