Lipid segregation explains selective toxicity of a series of fragments derived from the human cathelicidin LL-37

Antimicrobial Agents and Chemotherapy

Volumn 53, Issue 9, 2009, Pages 3705-3714

  Epand, Raquel F ^a   Wang, Guangshun ^b   Berno, Bob ^c   Epand, Richard M ^a  

^a MCMASTER UNIVERSITY MEDICAL CENTRE   (Canada)

^b UNIVERSITY OF NEBRASKA MEDICAL CENTER   (United States)

^c MCMASTER UNIVERSITY   (Canada)

Author keywords

[No Author keywords available]

Indexed keywords

CATHELICIDIN ANTIMICROBIAL PEPTIDE LL 37; PEPTIDE GF 17; PEPTIDE GF 17D3; PEPTIDE KR 12; PEPTIDE RI 10; UNCLASSIFIED DRUG;

AMINO ACID SEQUENCE; ANTIMICROBIAL ACTIVITY; ARTICLE; BACTERIAL MEMBRANE; CONTROLLED STUDY; GRAM NEGATIVE BACTERIUM; GRAM POSITIVE BACTERIUM; LIPID ANALYSIS; MINIMUM INHIBITORY CONCENTRATION; NONHUMAN; PHOSPHORUS NUCLEAR MAGNETIC RESONANCE; PRIORITY JOURNAL;

ANTIMICROBIAL CATIONIC PEPTIDES; CALORIMETRY, DIFFERENTIAL SCANNING; GRAM-NEGATIVE BACTERIA; HUMANS; MAGNETIC RESONANCE SPECTROSCOPY; MICROBIAL SENSITIVITY TESTS;

EID: 70349119495     PISSN: 00664804     EISSN: 10986596     Source Type: Journal    
DOI: 10.1128/AAC.00321-09     Document Type: Article

References (34)

1. Ames, B. N. 1966. Assay of inorganic phosphate, total phosphate and phosphatases. Methods Enzymol. 8:115-118.
2. Arouri, A., M. Dathe, and A. Blume. 2009. Peptide induced demixing in PG/PE lipid mixtures: a mechanism for the specificity of antimicrobial peptides towards bacterial membranes? Biochim. Biophys. Acta 1788:650-659.
3. Beining, P. R., E. Huff, B. Prescott, and T. S. Theodore. 1975. Characterization of the lipids of mesosomal vesicles and plasma membranes from Staphylococcus aureus. J. Bacteriol. 121:137-143.
4. Braff, M. H., M. A. Hawkins, N. A. Di, B. Lopez-Garcia, M. D. Howell, C. Wong, K. Lin, J. E. Streib, R. Dorschner, D. Y. Leung, and R. L. Gallo. 2005. Structure-function relationships among human cathelicidin peptides: dissociation of antimicrobial properties from host immunostimulatory activities. J. Immunol. 174:4271-4278.
5. Bucki, R., F. J. Byfield, and P. A. Janmey. 2007. Release of the antimicrobial peptide LL-37 from DNA/F-actin bundles in cystic fibrosis sputum. Eur. Respir. J. 29:624-632.
6. Epand, R. F., B. P. Mowery, S. E. Lee, S. S. Stahl, R. I. Lehrer, S. H. Gellman, and R. M. Epand. 2008. Dual mechanism of bacterial lethality for a cationic sequence-random copolymer that mimics host-defense antimicrobial peptides. J. Mol. Biol. 379:38-50.
7. Epand, R. F., M. A. Schmitt, S. H. Gellman, and R. M. Epand. 2006. Role of membrane lipids in the mechanism of bacterial species selective toxicity by two alpha/beta-antimicrobial peptides. Biochim. Biophys. Acta 1758:1343-1350.
8. Epand, R. F., M. Tokarska-Schlattner, U. Schlattner, T. Wallimann, and R. M. Epand. 2007. Cardiolipin clusters and membrane domain formation induced by mitochondrial proteins. J. Mol. Biol. 365:968-980.
9. Epand, R. M. 2007. Detecting the presence of membrane domains using DSC. Biophys. Chem. 126:197-200.
10. Epand, R. M., and R. F. Epand. 2009. Lipid domains in bacterial membranes and the action of antimicrobial agents. Biochim. Biophys. Acta 1788:289-294.
11. Epand, R. M., S. Rotem, A. Mor, B. Berno, and R. F. Epand. 2008. Bacterial membranes as predictors of antimicrobial potency. J. Am. Chem. Soc. 130:14346-14352.
12. Li, X., Y. Li, H. Han, D. W. Miller, and G. Wang. 2006. Solution structures of human LL-37 fragments and NMR-based identification of a minimal membrane-targeting antimicrobial and anticancer region. J. Am. Chem. Soc. 128:5776-5785.
13. Lopez-Garcia, B., W. Ubhayasekera, R. L. Gallo, and J. F. Marcos. 2007. Parallel evaluation of antimicrobial peptides derived from the synthetic PAF26 and the human LL37. Biochem. Biophys. Res. Commun. 356:107-113.
14. Matsuzaki, K. 1998. Magainins as paradigm for the mode of action of pore forming polypeptides. Biochim. Biophys. Acta 1376:391-400.
15. Mileykovskaya, E., A. C. Ryan, X. Mo, C. C. Lin, K. I. Khalaf, W. Dowhan, and T. A. Garrett. 2009. Phosphatidic acid and N-acylphosphatidylethanolamine form membrane domains in Escherichia coli mutant lacking cardiolipin and phosphatidylglycerol. J. Biol. Chem. 284:2990-3000.
16. Morein, S., A. Andersson, L. Rilfors, and G. Lindblom. 1996. Wild-type Escherichia coli cells regulate the membrane lipid composition in a "window" between gel and non-lamellar structures. J. Biol. Chem. 271:6801-6809.
17. Morgera, F., L. Vaccari, N. Antcheva, D. Scaini, S. Pacor, and A. Tossi. 2009. Primate cathelicidin orthologues display different structures and membrane interactions. Biochem. J. 417:727-735.
18. Nell, M. J., G. S. Tjabringa, A. R. Wafelman, R. Verrijk, P. S. Hiemstra, J. W. Drijfhout, and J. J. Grote. 2006. Development of novel LL-37 derived antimicrobial peptides with LPS and LTA neutralizing and antimicrobial activities for therapeutic application. Peptides 27:649-660.
19. Nizet, V., T. Ohtake, X. Lauth, J. Trowbridge, J. Rudisill, R. A. Dorschner, V. Pestonjamasp, J. Piraino, K. Huttner, and R. L. Gallo. 2001. Innate antimicrobial peptide protects the skin from invasive bacterial infection. Nature 414:454-457. (Pubitemid 34173727)
20. Oren, Z., J. C. Lerman, G. H. Gudmundsson, B. Agerberth, and Y. Shai. 1999. Structure and organization of the human antimicrobial peptide LL-37 in phospholipid membranes: relevance to the molecular basis for its non-cell-selective activity. Biochem. J. 341:501-513. (Pubitemid 29389178)
21. Ouhara, K., H. Komatsuzawa, T. Kawai, H. Nishi, T. Fujiwara, Y. Fujiue, M. Kuwabara, K. Sayama, K. Hashimoto, and M. Sugai. 2008. Increased resistance to cationic antimicrobial peptide LL-37 in methicillin-resistant strains of Staphylococcus aureus. J. Antimicrob. Chemother. 61:1266-1269.
22. Porcelli, F., R. Verardi, L. Shi, K. A. Henzler-Wildman, A. Ramamoorthy, and G. Veglia. 2008. NMR structure of the cathelicidin-derived human antimicrobial peptide LL-37 in dodecylphosphocholine micelles. Biochemistry 47:5565-5572. (Pubitemid 351705167)
23. Putsep, K., G. Carlsson, H. G. Boman, and M. Andersson. 2002. Deficiency of antibacterial peptides in patients with morbus Kostmann: an observation study. Lancet 360:1144-1149. (Pubitemid 35246490)
24. Schlattner, U., F. Gehring, N. Vernoux, M. Tokarska-Schlattner, D. Neumann, O. Marcillat, C. Vial, and T. Wallimann. 2004. C-terminal lysines determine phospholipid interaction of sarcomeric mitochondrial creatine kinase. J. Biol. Chem. 279:24334-24342.
25. Sieprawska-Lupa, M., P. Mydel, K. Krawczyk, K. Wojcik, M. Puklo, B. Lupa, P. Suder, J. Silberring, M. Reed, J. Pohl, W. Shafer, F. McAleese, T. Foster, J. Travis, and J. Potempa. 2004. Degradation of human antimicrobial peptide LL-37 by Staphylococcus aureus-derived proteinases. Antimicrob. Agents Chemother. 48:4673-4679.
26. Sigurdardottir, T., P. Andersson, M. Davoudi, M. Malmsten, A. Schmidtchen, and M. Bodelsson. 2006. In silico identification and biological evaluation of antimicrobial peptides based on human cathelicidin LL-37. Antimicrob. Agents Chemother. 50:2983-2989.
27. Strömstedt, A. A., M. Pasupuleti, A. Schmidtchen, and M. Malmsten. 2009. Evaluation of strategies for improving proteolytic resistance of antimicrobial peptides by using variants of EFK17, an internal segment of LL-37. Antimicrob. Agents Chemother. 53:593-602.
28. Turner, J., Y. Cho, N. N. Dinh, A. J. Waring, and R. I. Lehrer. 1998. Activities of LL-37, a cathelin-associated antimicrobial peptide of human neutrophils. Antimicrob. Agents Chemother. 42:2206-2214.
29. Wang, G. 2007. Determination of solution structure and lipid micelle location of an engineered membrane peptide by using one NMR experiment and one sample. Biochim. Biophys. Acta 1768:3271-3281.
30. Wang, G. 2007. Tool developments for structure-function studies of host defense peptides. Protein Pept. Lett. 14:57-69.
31. Wang, G., Y. Li, and X. Li. 2005. Correlation of three-dimensional structures with the antibacterial activity of a group of peptides designed based on a nontoxic bacterial membrane anchor. J. Biol. Chem. 280:5803-5811.
32. Wang, G. 2008. Structures of human host defense cathelicidin LL-37 and its smallest antimicrobial peptide KR-12 in lipid micelles. J. Biol. Chem. 283:32637-32643.
33. Wang, Z., and G. Wang. 2004. APD: the Antimicrobial Peptide Database. Nucleic Acids Res. 32:D590-D592.
34. Zanetti, M. 2004. Cathelicidins, multifunctional peptides of the innate immunity. J. Leukoc. Biol. 75:39-48.