A double TROSY hNCAnH experiment for efficient assignment of large and challenging proteins

Journal of the American Chemical Society

Volumn 131, Issue 36, 2009, Pages 12880-12881

  Frueh, Dominique P ^a   Arthanari, Haribabu ^a   Koglin, Alexander ^a,b   Walsh, Christopher T ^a   Wagner, Gerhard ^a  

^a HARVARD MEDICAL SCHOOL   (United States)

^b LOS ALAMOS NATIONAL LABORATORY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ALPHA CARBON; AMIDE PROTON; CHAIN ELONGATION; DATA SETS; NONRIBOSOMAL PEPTIDE SYNTHETASES; SEQUENTIAL CORRELATIONS; SIGNAL INTENSITIES; TRIPLE-RESONANCE;

AMIDES; AMINES; AMINO ACIDS; ORGANIC ACIDS; PROTEINS;

EXPERIMENTS;

AMIDE; BACTERIAL ENZYME; CARBON; DEUTERIUM OXIDE; ENTEROBACTIN SYNTHETASE; HYDROGEN; NITROGEN; PROTEIN; PROTON; UNCLASSIFIED DRUG;

AMINO ACID SEQUENCE; ARTICLE; CHEMICAL STRUCTURE; CORRELATION ANALYSIS; ESCHERICHIA COLI; HETERONUCLEAR SINGLE QUANTUM COHERENCE; NONHUMAN; NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY; PROTEIN STRUCTURE; SPECTROSCOPY; TRANSVERSE RELAXATION OPTIMIZED SPECTROSCOPY;

NUCLEAR MAGNETIC RESONANCE, BIOMOLECULAR; PROTEIN CONFORMATION; PROTEINS;

EID: 70349102122     PISSN: 00027863     EISSN: None     Source Type: Journal    
DOI: 10.1021/ja9046685     Document Type: Article

References (16)

1. (a) Sattler, M.; Schleucher, J.; Griesinger, C. Progr. NMR Spectrosc. 1999, 34, 93-158.
2. (b) Ferentz, A. E.; Wagner, G. Q. Rev. Biophys. 2000, 33, 29-65.
3. Weisemann, R.; Ruterjans, H.; Bermel, W. J. Biomol. NMR 1993, 3, 113-120
4. Panchal, S. C.; Bhavesh, N. S.; Hosur, R. V. J. Biomol. NMR 2001, 20, 135-147
5. (a) Grzesiek, S.; Bax, A. J. Am. Chem. Soc. 1993, 115, 4369.
6. (b) Matsuo, H.; Kupce, E.; Li, H.; Wagner, G. J. Magn. Reson. B 1996, 111, 194-198
7. (c) Bracken, C.; Palmer, A. G., 3rd; Cavanagh, J. J. Biomol. NMR 1997, 9, 94-100.
8. Sun, Z. Y.; Frueh, D. P.; Selenko, P.; Hoch, J. C.; Wagner, G. J. Biomol. NMR 2005, 33, 43-50.
9. Frueh, D. P.; Arthanari, H.; Wagner, G. J. Biomol. NMR 2005, 33, 187-196
10. Frueh, D. P.; Sun, Z. Y.; Vosburg, D. A.; Walsh, C. T.; Hoch, J. C.; Wagner, G. J. Am. Chem. Soc. 2006, 128, 5757-5763.
11. (a) Frueh, D. P.; Arthanari, H.; Koglin, A.; Vosburg, D. A.; Bennett, A. E.; Walsh, C. T.; Wagner, G. Nature 2008, 454, 903-906
12. (b) Koglin, A.; Lohr, F.; Bernhard, F.; Rogov, V. V.; Frueh, D. P.; Strieter, E. R.; Mofid, M. R.; Guntert, P.; Wagner, G.; Walsh, C. T.; Marahiel, M. A.; Dotsch, V. Nature 2008, 454, 907-911
13. Gehring, A. M.; Bradley, K. A.; Walsh, C. T. Biochemistry 1997, 36, 8495-8503
14. Drake, E. J.; Nicolai, D. A.; Gulick, A. M. Chem. Biol. 2006, 13, 409-419
15. Rovnyak, D.; Frueh, D. P.; Sastry, M.; Sun, Z. Y.; Stern, A. S.; Hoch, J. C.; Wagner, G. J. Magn. Reson. 2004, 170, 15-21.
16. Takeuchi, K.; Frueh, D.; Hyberts, S.; Sun, Z. Y.; Wagner, G. In preparation.