A novel, label-free immunosensor for the detection of α-fetoprotein using functionalised gold nanoparticles

Clinical Biochemistry

Volumn 42, Issue 15, 2009, Pages 1524-1530

  Liang, Wenbin ^a,b   Yi, Weijing ^b   Li, Shuhui ^b   Yuan, Ruo ^a   Chen, An ^b   Chen, Sha ^b   Xiang, Guiming ^b   Hu, Chuanmin ^b  

^a SOUTHWEST UNIVERSITY   (China)

^b THIRD MILITARY MEDICAL UNIVERSITY   (China)

Author keywords

1 fetoprotein; Functional gold nanoparticle; Immunosensor; Sulfhyrdryl viologen

Indexed keywords

ALPHA FETOPROTEIN; ALPHA FETOPROTEIN ANTIBODY; CARBON; GOLD NANOPARTICLE; VIOLOGEN;

ARTICLE; CLINICAL ARTICLE; CONTROLLED STUDY; CORRELATION COEFFICIENT; COST EFFECTIVENESS ANALYSIS; COVALENT BOND; ELECTRODE; HUMAN; IMMUNOASSAY; IMMUNOSENSOR; PRIORITY JOURNAL; PROTEIN DETERMINATION; SIGNAL NOISE RATIO; SYNTHESIS;

ALPHA-FETOPROTEINS; BIOSENSING TECHNIQUES; CARBON; ELECTROCHEMISTRY; ELECTRODES; GOLD; HUMANS; IMMUNOASSAY; METAL NANOPARTICLES; MOLECULAR STRUCTURE; SENSITIVITY AND SPECIFICITY; SURFACE PROPERTIES;

EID: 70349088554     PISSN: 00099120     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.clinbiochem.2009.07.009     Document Type: Article

References (31)

1. Santandreu M., Cespedes F., Alegret S., and Martinez-Fabregas E. Amperometric immunosensor based on rigid conducting immunocomposites. Anal. Chem. 69 (1997) 2080-2085
2. Wu L.N., Yan F., and Ju H.X. An amperometric immunosensor for separation-free immunoassay of CA125 based on its covalent immobilization coupled with thionine on carbon nanofiber. J. Immunol. Methods 322 (2007) 12-19
3. Dai Z., Yan F., Yu H., Hu X.Y., and Ju H.X. Novel amperometric immunosensor for rapid separation-free immunoassay of carcinoembryonic antigen. J. Immunol. Methods 287 (2004) 13-20
4. Tsutsumi H., Takeoka K., and Oishi T. Preparation and characterization of new self-assembled polyamide film containing disulfide bonds on gold electrode. J. Colloid Interface Sci. 185 (1997) 432-435
5. Storri S., Santoni T., Minunni M., and Mascini M. Surface modifications for the development of piezoimmunosensors. Biosens. Bioelec-tron 13 (1998) 347-357
6. Hianik T., Snejdarkova M., Sokolikova L., et al. Immunosensors based on supported lipid membranes, protein films and liposomes modified by antibodies. Sens. Actuators B. 57 (1999) 201-212
7. Zhuo Y., Yuan R., Chai Y.Q., et al. A reagentless amperometric immunosensor based on gold nanoparticles/thionine/Nafion-membrane-modified gold electrode for determination of a-1-fetoprotein. Electrochem. Commun. 7 (2005) 355-360
8. Zhuo Y., Yuan R., Chai Y.Q., et al. Amperometric enzyme immunosensors based on layer-by-layer assembly of gold nanoparticles and thionine on Nafion modified electrode surface for a -1-fetoprotein determinations. Sens. Actuators B. 114 (2006) 631-639
9. Darain F., Park S.U., and Shim Y.B. Disposable amperometric immunosensor system for rabbit IgG using a conducting polymer modified screen-printed electrode Biosens. Bioelectron 18 (2003) 773-780
10. Dai Z., Yan F., Yu H., Hu X.Y., and Ju H.X. Novel amperometric immunosensor for rapid separation-free immunoassay of carcinoembryonic antigen. J. Immun. Meth. 287 (2004) 13-20
11. Yu H., Yan F., Dai Z., and Ju H.X.A. A disposable amperometric immunosensor for α-1-fetoprotein based on enzyme-labeled antibody/chitosan-membrane-modified screen-printed carbon electrode. Anal. Biochem. 331 (2004) 98-105
12. Ruan C.M., Yang L.J., and Li Y.B. Immunosensor clips for detection of Escherichia coli 0157:H7 using electrochemical impedance spectroscopy. Anal. Chem. 74 (2002) 4814-4820
13. 2 phytate. Langmuir 19 (2003) 4327-4331
14. 2 nanoparticle films on pyrolytic graphite electrodes: direct electrochemistry and bioelectrocatalysis. Electrochim. Acta 49 (2004) 1981-1988
15. Li J.S., Wu Z.S., Wang H., Shen G.L., and Yu R.Q.A. A mild hydrophobic interaction chromatography involving polyethylene glycol immobilized to agarose media refolding recombinant Staphylococcus aureus elongation factor G. Sens. Actuators, B. 110 (2005) 327-335
16. Moulton S.E., Barisci J.N., Bath A., Stella R., and Wallace G.G. Investigation of Ig.G adsorption and the effect on electrochemical responses at titanium dioxide electrode. Langmuir 21 (2005) 316-322
17. Liang W.B., Yuan R., Chai Y.Q., Li Y., and Zhuo Y.A. A novel label-free voltammetric immunosensor for the detection of α-fetoprotein using functional titanium dioxide nanoparticles. Electrochim. Acta 53 (2008) 2302-2308
18. Shi Y.T., Yuan R., Chai Y.Q., Tang M.Y., and He X.L. Amplification of antigen-antibody interactions via back-filling of HRP on the layer-by-layer self-assembling of thionine and gold nanoparticles films on titania nanoparticles/gold nanoparticles-coated Au electrode. J. Electroanal. Chem. 604 (2007) 9-16
19. Hosono H., and Kaneko M. Effect of methylene chain length on electron transfer and photocurrent generation using viologen-linked porphyrin monolayer modified ITO electrode. J. Photochem. Photobiol. A. 107 (1997) 63-70
20. Alpert E., Hershberg R., Schur P.H., and Lsselbacher K.J. a-fetoprotein in human hepatoma: improved detection in serum, and quantitative studies using a new sensitive technique. Gastroenterology 61 (1971) 137-143
21. Okuda K. Primary liver cancer. Quadriennal review lecture. Dig. Dis. Sci. 31 (1986) 133-146
22. Okuda K., Kotoda K., Obata H., et al. Clinical observations during a relatively early stage of hepatocellular carcinoma, with special reference to serum alpha-fetoprotein levels. Gastroenterology 69 (1975) 226-234
23. Nomura F., Ohnishi K., and Tanabe Y. Clinical features and prognosis of hepatocellular carcinoma with reference to serum alpha-fetoprotein levels. Analysis of 606 patients. Cancer 64 (1989) 1700-1707
24. Oka H., Tamori A., Kuroki T., Kobayashi K., and Yamamotc S. Prospective study of α-fetoprotein in cirrhotic patients monitored for development of hepatocellular carcinoma. Hepatology 19 (1994) 61-66
25. Stefanova I., Horejisi V., Kristofova H., Angelisova P., Zizkovsky V., and Hilgert I. Monoclonal antibodies against human α-fetoprotein. Exploitation of an unusual calcium-dependent interaction with the antigen for analytical and preparative purposes. J. Immun. Meth. 111 (1988) 67-73
26. Vermeulen L.A., and Thompson M.E. Stable photoinduced charge separation in layered viologen compounds. Lett. Nat. 358 (1992) 656
27. Katz E., and Willner I. Probing biomolecular interactions at conductive and semiconductive surfaces by impedance spectroscopy: routes to impedimetric immunosensors, DNA-sensors, and enzyme biosensors. Electroanalysis 15 (2003) 913-947
28. Pardo-Yissar V., Katz E., Wasserman J., and Willner I. Acetylcholine esterase-labeled CdS nanoparticles on electrodes: photoelectrochemical sensing of the enzyme inhibitors. J. Am. Chem. Soc. 125 (2003) 622-623
29. Bardea A., Katz E., and Willner I. Probing antigen-antibody interactions on electrode supports by the biocatalyzed precipitation of an insoluble product. Electroanalysis 12 (2000) 1097-1106
30. Chen Z.P., Jiang J.H., Shen G.L., and Yu Y.Q. Impedance immunosensor based on receptor protein adsorbed directly on porous gold film. Analytica Chimica Acta 553 (2005) 190-195
31. Chen X.J., Wang Y.Y., Zhou J.J., Yan W., Li X.i.n.g.h.u.a., and Zhu J.J. Electrochemical impedance immunosensor based on three-dimensionally ordered macroporous gold film. Anal. Chem. 80 (2008) 2133-2140