Isolation of an alkane-degrading Alcanivorax sp. strain 2B5 and cloning of the alkB gene

Bioresource Technology

Volumn 101, Issue 1, 2010, Pages 310-316

  Liu, Yi Chen ^a   Li, Ling Zhi ^a   Wu, Ying ^a   Tian, Wei ^a   Zhang, Li Ping ^a   Xu, Lian ^a   Shen, Qi Rong ^a   Shen, Biao ^a  

^a NANJING AGRICULTURAL UNIVERSITY   (China)

Author keywords

Alcanivorax sp.; alkB gene; Biodegradation; Isolation and identification

Indexed keywords

16S RRNA GENE SEQUENCE; ALCANIVORAX SP.; ALKANE HYDROXYLASE; ALKB GENE; AMINO ACID SEQUENCE; BRANCHED ALKANES; CRUDE OIL; DONGHAI; HETEROLOGOUS EXPRESSION; HYDROXYLASES; ISOLATION AND IDENTIFICATION; LONG CHAIN ALKANE; N-ALKANES; NACL CONCENTRATION; OCTADECANE; OPTIMAL TEMPERATURE; PHYSIOLOGICAL CHARACTERISTICS; PRISTANE; PSEUDOMONAS FLUORESCENS; RT-PCR ANALYSIS; SOLE CARBON SOURCE;

AMINES; AMINO ACIDS; BIOCHEMISTRY; BIODEGRADATION; CLONING; CRUDE PETROLEUM; GENES; MICROBIOLOGY; ORGANIC ACIDS; PARAFFINS; SODIUM CHLORIDE;

DEGRADATION;

ALKANE; ALKANE HYDROXYLASE; ALKB PROTEIN; ALKB1 PROTEIN; BACTERIAL PROTEIN; OCTADECANE; OXYGENASE; PETROLEUM; PRISTANE; RNA 16S; SODIUM CHLORIDE; UNCLASSIFIED DRUG;

ALKANE; BIODEGRADATION; CONCENTRATION (COMPOSITION); ISOLATION EFFECT; MICROBIAL ACTIVITY; PHYSIOLOGY; TEMPERATURE EFFECT;

ALCANIVORAX; ALCANIVORAX BORKUMENSIS; ARTICLE; BACTERIAL GROWTH; BACTERIAL STRAIN; BACTERIUM IDENTIFICATION; BACTERIUM ISOLATION; CARBON SOURCE; CHINA; CONCENTRATION RESPONSE; CONTROLLED STUDY; GENE EXPRESSION; HETEROLOGOUS EXPRESSION; NONHUMAN; NUCLEOTIDE SEQUENCE; PH; PRIORITY JOURNAL; PSEUDOMONAS FLUORESCENS; SEA POLLUTION; SEQUENCE ANALYSIS; SEQUENCE HOMOLOGY; TEMPERATURE DEPENDENCE;

ALCANIVORACEAE; ALKANES; CLONING, MOLECULAR; ESCHERICHIA COLI PROTEINS; MIXED FUNCTION OXYGENASES; RECOMBINANT PROTEINS; SPECIES SPECIFICITY;

ASIA; CHINA; DONGHAI [JIANGSU]; EURASIA; FAR EAST; JIANGSU;

ALCANIVORAX; ALCANIVORAX BORKUMENSIS; NEGIBACTERIA; PSEUDOMONAS; PSEUDOMONAS FLUORESCENS;

EID: 70249108475     PISSN: 09608524     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.biortech.2009.08.028     Document Type: Article

References (27)

1. Cappello S., Denaro R., Genovese M., Giuliano L., and Yakimov M.M. Predominant growth of Alcanivorax during experiments on "oil spill bioremediation" in mesocosms. Microbiol. Res. 162 (2007) 185-190
2. Dyksterhouse S.E., Gray J.P., Herwig R.P., Lara J.C., and Staley J.T. Cycloclasticus pugetii gen. nov., sp. nov., an aromatic hydrocarbon-degrading bacterium from marine sediments. Int. J. Syst. Bacteriol. 45 (1995) 116-123
3. Engelhardt M.A., Daly K., Swannell R.P.J., and Head I.M. Isolation and characterization of a novel hydrocarbon-degrading, gram-positive bacterium, isolated from intertidal beach sediment, and description of Planococcus alkanoclasticus sp. Nov. J. Appl. Microbiol. 90 (2001) 237-247
4. Gauthier M.J., Lafay B., Christen R., Fernandez L., Acquaviva M., Bonin P., and Bertrand J.C. Marinobacter hydrocarbonoclasticus gen. nov., sp. nov., a new, extremely halotolerant, hydrocarbon-degrading marine bacterium. Int. J. Syst. Bacteriol. 42 4 (1992) 568-576
5. Golyshin P.N., Chernikova T.N., Abraham W.R., Lünsdorf H., Timmis K.N., and Yakimov M.M. Oleiphilaceae fam. nov., to include Oleiphilus messinensis gen. nov., sp. nov., a novel marine bacterium that obligatory utilizes hydrocarbons. Int. J. Syst. Evol. Microbiol. 52 (2002) 901-911
6. Harayama S., Kishira H., Kasai Y., and Shutsubo K. Petroleum biodegradation in marine environments. J. Mol. Microbiol. Biotechnol. 1 (1999) 63-70
7. Hedlund B.P., Geiselbrecht A.D., Bair T.J., and Staley J.T. Polycyclic aromatic hydrocarbon degradation by a new marine bacterium, Neptunomonas naphthovorans gen. nov., sp. nov. Appl. Environ. Microbiol. 65 (1999) 251-259
8. Højberg O., Schnider U., Winteler H.V., Sørensen J., and Haas D. Oxygen-sensing reporter strain of Pseudomonas fluorescens for monitoring the distribution of low-oxygen habitats in soil. Appl. Environ. Microbiol. 65 9 (1999) 4085-4093
9. Holt J.G., Krieg N.R., Sneath P.H.A., Staley J.T., and Williams S.T. Bergey's Manual of Determinative Bacteriology. ninth ed. (1994), Wilkins, USA
10. Kasai Y., Kishira H., and Harayama S. Bacteria belonging to the genus Cycloclasticus play a primary role in the degradation of aromatic hydrocarbons released in a mae environment. Appl. Environ. Microbiol. 68 (2002) 5625-5633
11. Kasai Y., Kishira H., Sasaki T., Syutsubo K., Watanabe K., and Harayama S. Predominant growth of Alcanivorax strains in oil-contaminated and nutrient-supplemented seawater. Environ. Microbiol. 4 (2002) 141-147
12. Lageveen R.G., Huisman G.W., Preusting H., Ketelaar P., Eggink G., and Witholt B. Formation of polyesters by Pseudomonas oleovorans: effect of substrates on formation and composition of poly-(R)-3-hydroxyalkanoates and poly-(R)-3-hydroxyalkenoates. Appl. Environ. Microbiol. 54 12 (1988) 2924-2932
13. Lane D.J. 16S/23S rRNA sequencing. In: Stackebrandt E., and Goodfellow M. (Eds). Nucleic Acid Techniques in Bacterial Systematics (1991), Chichester, England 115-175
14. Liu C., and Shao Z. Alcanivorax dieselolei sp. nov., a novel alkane-degrading bacterium isolated from sea water and deep-sea sediment. Int. J. Syst. Evol. Microbiol. 55 (2005) 1181-1186
15. Marin M., Pedregosa A., Rios S., and Laborda F. Study of factors influencing the degradation of heating oil by Acinetobacter calcoaceticus MM5. Int. Biodeter. Biodegr. 33 (1996) 69-75
16. Miller S.A., Dykes D.D., and Polesky H.F. A simple salting out procedure for extracting DNA from human nucleated cells. Nucleic Acid Res. 16 (1988) 1215
17. Sambrook J., Fritsch E.F., and Maniatis T. Molecular Cloning: A Laboratory Manual. second ed. (1989), Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York pp. 908-910
18. Schneiker S., et al. Genome sequence of the ubiquitous hydrocarbon-degrading marine bacterium Alcanivorax borkumensis. Nat. Biotechnol. 24 8 (2006) 997-1004
19. Shanklin J., Whittle E., and Fox B.G. Eight histidine residues are catalytically essential in a membrane-associated iron enzyme, stearoyl-CoA desaturase, and are conserved in alkane hydroxylase and xylene monooxygenase. Biochemistry 33 43 (1994) 12787-12794
20. Smits T.H.M., Röthlisberger M., Witholt B., and van Beilen J.B. Molecular screening for alkane hydroxylase genes in gram-negative and gram-positive strains. Environ. Microbiol. 1 4 (1999) 307-317
21. Smits T.H.M., Seeger M.A., Witholt B., and van Beilen J.B. New alkane-responsive expression vectors for E. coli and Pseudomonas. Plasmid 46 (2001) 16-24
22. Smits T.H.M., Balada S.B., Witholt B., and van Beilen J.B. Functional analysis of alkane hydroxylases from gram-negative and gram-positive bacteria. J. Bacteriol. 184 (2002) 1733-1742
23. van Beilen J.B., Wubbolts M.G., and Witholt B. Genetics of alkane oxidation by Pseudomonas oleovorans. Biodegradation 5 (1994) 161-174
24. van Beilen J.B., Smits T.H.M., Whyte L.G., Schorcht S., Röthlisberger M., Plaggemeier T., Engesser K.H., and Witholt B. Alkane hydroxylase homologues in gram-positive strains. Environ. Microbiol. 4 (2002) 676-682
25. Wang S., He J., Cui Z., and Li S. Self-formed adaptor PCR: a simple and efficient method for chromosome walking. Appl. Environ. Microbiol. 73 15 (2007) 5048-5051
26. Yakimov M.M., Golyshin P.N., Lang S., Moore E.R., Abraham W.R., Lünsdorf H., and Timmis K.N. Alcanivorax borkumensis gen. nov., sp. nov., a new, hydrocarbon-degrading and surfactant-producing marine bacterium. Int. J. Syst. Bacteriol. 48 (1998) 339-348
27. Yakimov M.M., Giuliano L., Gentile G., Crisafi E., Chernikova T.N., Abraham W.R., Lünsdorf H., Timmis K.N., and Golyshin P.N. Oleispira antarctica gen nov., sp. nov., a novel hydrocarbonoclastic marine bacterium isolated from Antarctic coastal sea water. Int. J. Syst. Evol. Microbiol. 53 (2003) 779-785