Signal transduction pathway involved in the ex vivo expansion of limbal epithelial cells cultured on various substrates

Indian Journal of Medical Research

Volumn 129, Issue 4, 2009, Pages 382-389

  Sudha, Balasubramanian ^a   Jasty, Srilatha ^a   Krishnan, Sasirekha ^a   Krishnakumar, Subramanian ^a  

^a VISION RESEARCH FOUNDATION   (India)

Author keywords

Chitosan matrices; Denuded human amniotic membrane; Limbal stem cells; PI3K Akt JNK pathway; Signal pathway

Indexed keywords

BETA ACTIN; CHITOSAN; GOLD; MITOGEN ACTIVATED PROTEIN KINASE; PHOSPHATIDYLINOSITOL 3 KINASE; PROTEIN KINASE B; PROTEIN P42; PROTEIN P44; SERINE; SILVER; STRESS ACTIVATED PROTEIN KINASE; SYNAPTOPHYSIN; THREONINE; TRANSCRIPTION FACTOR FKHRL1; NERVE GROWTH FACTOR;

AMNION; ARTICLE; CELL CULTURE; CONTROLLED STUDY; CORNEA LIMBUS; ENZYME ACTIVATION; ENZYME ACTIVITY; ENZYME INHIBITION; ENZYME SUBSTRATE COMPLEX; EPITHELIUM CELL; EX VIVO STUDY; EXPLANT; HUMAN; HUMAN CELL; HUMAN TISSUE; SIGNAL TRANSDUCTION; STEM CELL; STEM CELL EXPANSION; WESTERN BLOTTING; CELL PROLIFERATION; CORNEA; CYTOLOGY; METABOLISM; PHYSIOLOGY;

1-PHOSPHATIDYLINOSITOL 3-KINASE; AMNION; BLOTTING, WESTERN; CELL PROLIFERATION; CELLS, CULTURED; CHITOSAN; CORNEA; EPITHELIAL CELLS; HUMANS; NERVE GROWTH FACTOR; SIGNAL TRANSDUCTION; STEM CELLS;

EID: 70149119531     PISSN: 09715916     EISSN: 09715916     Source Type: Journal    
DOI: None     Document Type: Article

References (30)

1. Tsai RJ, Li LM, Chen JK. Reconstruction of damaged corneas by transplantation of autologous limbal epithelial cells. N Engl J Med 2000; 343: 86-93.
2. Koizumi N, Inatomi T, Suzuki T, Sotozono C, Kinoshita S. cultivated corneal epithelial transplantation for ocular surface reconstruction in acute phase of Stevens-Johnson syndrome. Arch Ophthal mol 2001; 119: 298-300.
3. Koizumi N, Inatomi T, Suzuki T, Sotozono C, Kinoshita S. Cultivated corneal epithelial stem cell transplantation in ocular surface disorders. Ophthalmology 2001; 108: 1569-74.
4. Touhami A, Grueterich M, Tseng SC. The role of NGF signaling in human limbal epithelium expanded by amniotic membrane culture. Invest Ophthalmol Vis Sci 2002; 43: 987-94.
5. Ti SE, Anderson D, Touhami A, Kim C, Tseng SC. Factors affecting outcome following transplantation of ex vivo expanded limbal epithelium on amniotic membrane for total limbal deficiency in rabbits. Invest Ophthalmol Vis Sci 2002; 43: 2584-92.
6. Ti SE, Grueterich M, Espana EM, Touhami A, Anderson DF, Tseng SC. Correlation of long term phenotypic and clinical outcomes following limbal epithelial transplantation cultivated on amniotic membrane in rabbits. Br J Ophthalmol 2003; 88: 422-7.
7. Espana EM, Ti SE, Grueterich M, Touhami A, Tseng SC. Corneal stromal changes following reconstruction by ex vivo expanded limbal epithelial cells in rabbits with total limbal stem cell deficiency. Br J Ophthalmol 2003; 87: 1509-14.
8. Meller D, Dabul V, Tseng SC. Expansion of conjunctival epithelial progenitor cells on amniotic membrane. Exp Eye Res 2002; 74: 537-45.
9. Hernandez GEE, Theiss C, Steuhl KP, Meller D. Gap junctional communication in microinjected human limbal and peripheral corneal epithelial cells cultured on intact amniotic membrane. Exp Eye Res 2003; 76: 303-14.
10. Tseng SCG, Tsubota K. Amniotic membrane transplantation for ocular surface reconstruction. In: Holland EJ, Marris MJ, editors. Ocular surface diseases: Medical and surgical management. New York: Springer Verlag; 2002 p. 226-31.
11. Zheng WH, Kar S, Quirion R. FKHRL1 and its homologs are new targets of nerve growth factor Trk receptor signaling. J Neurochem 2002; 80: 1049-61.
12. Datta SR, Brunet A, Greenberg ME. Cellular survival: a play in three Akts. Genes Dev 1999; 13: 2905-27.
13. Johnson GL, Lapadat R. Mitogen-activated protein kinase pathways mediated by ERK, JNK, and p38 protein kinases. Science 2002; 298: 1911-2.
14. Graves JD, Draves KE, Craxton A, Saklatvala J, Krebs EG, Clark EA. Involvement of stress activated protein kinase and p38 mitogen-activated protein kinase in mIgM-induced apoptosis of human B-lymphocytes. Proc Natl Acad Sci USA 1996; 93: 13814-8.
15. Xia Z, Dickens M, Raingeaud J, Davis RJ, Greenberg ME. Opposing effects of ERK and JNK-p38 MAP kinases on apoptosis. Science 1995; 270: 1326-31.
16. Guillermo PJ, William RS. PI3K/PTEN/AKT pathway. A critical mediator of oncogenic signaling. Cancer Treat Res 2003; 115: 145-67.
17. Yeh LK, Chen YH, Chiu CS, Hu FR, Young TH, Wang IJ. The phenotype of bovine corneal epithelial cells on chitosan membrane. Invest Ophthalmol Vis Sci 2007: 48: 1875.
18. Zhu X, Beuerman RW, Chan-Park MB, Cheng Z, Ang LP, Tan DT. Enhancement of the mechanical and biological properties of a biomembrane for tissue engineering the ocular surface. Ann Acad Med Singapore 2006; 35: 210-4.
19. Ang LP, Cheng ZY, Beuerman RW, Teoh SH, Zhu X, Tan DT. The development of a serum-free derived bioengineered conjunctival epithelial equivalent using an ultra thin poly (epsilon-caprolactone) membrane substrate. Invest Ophthalmol Vis Sci 2006; 47: 105-12.
20. Alaminos M, Del Carmen Sänchez-Quevedo M, Muñoz-Avila JI, Serrano D, Medialdea S, Carreras I, et al. Construction of a complete rabbit cornea substitute using a fibrin-agarose scaffold. Invest Opthalmol Vis Sci 2006; 47: 3311-7.
21. Duan X, McLaughlin C, Griffith M, Sheardown H. Biofunctionalization of collagen for improved biological response: scaffolds for corneal tissue engineering. Biomaterials 2007; 28: 78-88.
22. He H, Cho HT, Li W, Kawakita T, Jong L, Tseng SC. Signaling-transduction pathways required for ex vivo expansion of human limbal explants on intact amniotic membrane. Invest Ophthalmol Vis Sci 2006; 47: 151-7.
23. Lee SH, Tseng SC. Amniotic membrane transplantation for persistent epithelial defects with ulceration. Am J Ophthalmol 1997; 123: 303-12.
24. Kops GJ, Burgering BM. Forkhead transcription factors are targets of signalling by the proto-oncogene PKB (C-AKT). J Anat 2000; 197: 571-4.
25. Lowry OH, Rosenbrough NJ, Farr AL, Randall RJ. Protein measurement with the folin phenol reagent. J Biol Chem 1951; 193: 265-75.
26. Franke TF, Hornik CP, Segev L, Shostak GA, Sugimoto C. PI3K/Akt and apoptosis: size matters. Oncogene 2003; 22: 8983-98.
27. Rende M, Brizi E, Conner J, Treves S, Censier K, Provenzano C, et al. Nerve growth factor (NGF) influences differentiation and proliferation of myogenic cells in vitro via TrKA. Int J Develop Neurosci 2000; 18: 869-85.
28. Aplin AE, Howe A, Alahari SK, Juliano RL. Signal transduction and signal modulation by cell adhesion receptors: The role of integrins, cadherins, immunoglobulin-cell adhesion molecules, and selectins. Pharm Rev 1998; 50: 197-64.
29. You L, Kruse FE, Völcker HE. Neurotrophic factors in the human cornea. Invest Ophthalmol Vis Sci 2000; 41: 692-702.
30. Fruman DA, Meyers RE, Cantley LC. Phosphoinositide kinases. Annu Rev Biochem 1998; 67: 481-507.