Flow injection analysis electrospray ionization mass spectrometry for high-throughput screening of a gene library for amidase activity

Analytical Biochemistry

Volumn 394, Issue 2, 2009, Pages 159-163

  Henderickx, Huub J W ^a   Raemakers Franken, Petronella C ^b   van der Wal, Sjoerd ^a   de Koster, Chris G ^c   Duchateau, Alexander L L ^b   Sonke, Theo ^b  

^a DSM RESOLVE   (Netherlands)

^b DSM Pharmaceutical Products   (Netherlands)

^c UNIVERSITY OF AMSTERDAM   (Netherlands)

Author keywords

Amidase activity; Biocatalyst screening; FIA ESI MS; Mycobacterium neoaurum; Selected ion monitoring

Indexed keywords

AMIDES; AMINO ACIDS; CLONING; DRUG PRODUCTS; GENES; MASS SPECTROMETRY; NEGATIVE IONS;

AMIDASE; CALIBRATION PROCEDURE; ELECTROSPRAY IONIZATION MASS SPECTROMETRY; FIA-ESI-MS; FLOW-INJECTION ANALYSIS; HIGH THROUGHPUT SCREENING; MYCOBACTERIUM NEOAURUM; SELECTED ION MONITORING;

ELECTROSPRAY IONIZATION;

AMIDASE; AMIDE; AMINO ACID; LEUCINE; METHYL GROUP;

ANALYTIC METHOD; ARTICLE; CALIBRATION; CONTROLLED STUDY; DILUTION; ELECTROSPRAY MASS SPECTROMETRY; ENZYME ACTIVITY; FLOW INJECTION ANALYSIS; GENE LIBRARY; HIGH THROUGHPUT SCREENING; IONIZATION; MYCOBACTERIUM; PRIORITY JOURNAL; QUADRUPOLE MASS SPECTROMETRY; QUANTITATIVE ANALYSIS;

MYCOBACTERIUM NEOAURUM;

EID: 70049103220     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2009.07.018     Document Type: Article

References (18)

1. A. Kleemann, J. Engel, D. Reichert, B. Kutscher, Pharmaceutical Substances: Syntheses, Patents, Applications, Thieme, Stuttgart, Germany, 1999.
2. Reinhold D.F., Firestone R.A., Gaines W.A., Chemerda J.M., and Sletzinger M. Synthesis of l-α-methyldopa from asymmetric intermediates. J. Org. Chem. 33 (1968) 1209-1213
3. Toniolo C., and Benedetti E. Structures of polypeptides from α-amino acids disubstituted at the α-carbon. Macromolecules 24 (1991) 4004-4009
4. Sonke T., Kaptein B., Boesten W.H.J., Broxterman Q.B., Kamphuis J., Formaggio F., Toniolo C., Rutjes F.P.J.T., and Schoemaker H.E. Aminoamidase-catalyzed preparation and further transformations of enantiopure α-hydrogen- and α,α-disubstituted α-amino acids. In: Patel R.N. (Ed). Stereoselective Biocatalysis (2000), Marcel Dekker, New York 23-58
5. Kaptein B., Boesten W.H.J., Broxterman Q.B., Peters P.J.H., Schoemaker H.E., and Kamphuis J. Enzymatic resolution of α,α-disubstituted α-amino acid esters and amides. Tetrahedron 4 (1993) 1113-1116
6. Kruizinga W.H., Bolster J., Kellogg R.M., Kamphuis J., Boesten W.H.J., Meijer E.M., and Schoemaker H.E. Synthesis of optically pure α-alkylated α-amino acids and a single-step method for enantiomeric excess determination. J. Org. Chem. 53 (1988) 1826-1827
7. Hermes H.F.M., Tandler R.F., Sonke T., Dijkhuizen L., and Meijer E.M. Purification and characterization of an l-amino amidase from Mycobacterium neoaurum ATCC 25795. Appl. Environ. Microbiol. 60 (1994) 153-159
8. Sonke T., Duchateau A.L.L., Schipper D., Euverink G.J.W., van der Wal S., Henderickx H.J.W., Bezemer R., and Vollebregt A. Industrial perspectives on assays. In: Reymond J.-L. (Ed). Enzyme Assays: High-Throughput Screening, Genetic Selection, and Fingerprinting (2006), Wiley-VCH, Weinheim, Germany 95-135
9. Wahler D., and Reymond J.-L. Novel methods for biocatalyst screening. Curr. Opin. Chem. Biol. 5 (2001) 152-158
10. Tholey A., and Heinzle E. Methods for biocatalyst screening. Adv. Biochem. Eng. Biotechnol. 74 (2002) 1-19
11. Reymond J.-L., and Babiak P. Screening systems. Adv. Biochem. Eng. Biotechnol. 105 (2007) 31-58
12. In: Reymond J.-L. (Ed). Enzyme Assays: High-Throughput Screening, Genetic Selection, and Fingerprinting (2006), Wiley-VCH, Weinheim, Germany
13. Preisig C., and Byng G. Applications of mass spectrometry in screening for new biocatalysts. J. Mol. Catal. B 11 (2001) 733-741
14. S.A. Raillard, Y.H. Chen, C. Krebber, High Throughput Mass Spectrometry, Patent WO 00/48004 (to Maxygen), 2000.
15. Kamphuis J., Hermes H.F.M., van Balken J.A.M., Schoemaker H.E., Boesten W.H.J., and Meijer E.M. Chemo-enzymatic synthesis of enantiomerically pure α-H and α-alkyl-α-amino acids and derivatives. In: Lubec G., and Rosenthal G.A. (Eds). Amino Acids: Chemistry, Biology, and Medicine (1990), ESCOM Science, Leiden, The Netherlands 119-125
16. Sonke T., Ernste S., Tandler R.F., Kaptein B., Peeters W.P.H., van Assema F.B.J., Wubbolts M.G., and Schoemaker H.E. l-Selective amidase with extremely broad substrate specificity from Ochrobactrum anthropi NCIMB 40321. Appl. Environ. Microbiol. 71 (2005) 7961-7973
17. Wang T., Zeng L., Strader T., Burton L., and Kassel D.B. A new ultra-high-throughput method for characterizing combinatorial libraries incorporating a multiple probe autosampler coupled with flow injection mass spectrometry analysis. Rapid Commun. Mass Spectrom. 12 (1998) 1123-1129
18. Morand K.L., Burt T.M., Regg B.T., and Chester T.L. Techniques for increasing the throughput of flow injection mass spectrometry. Anal. Chem. 73 (2001) 247-252