Use of tissue-engineered skin to study in vitro biofilm development

Dermatologic Surgery

Volumn 35, Issue 9, 2009, Pages 1334-1341

  Charles, Carlos A ^a   Ricotti, Carlos A ^a   Davis, Stephen C ^a   Mertz, Patricia M ^a   Kirsner, Robert S ^a  

^a UNIVERSITY OF MIAMI MILLER SCHOOL OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ARTICLE; BACTERIUM ISOLATE; BIOFILM; CONTROLLED STUDY; EPIFLUORESCENCE MICROSCOPE; HUMAN; HUMAN CELL; IN VITRO STUDY; NONHUMAN; PRIORITY JOURNAL; PSEUDOMONAS AERUGINOSA; SKIN BIOPSY; SKIN CULTURE; SKIN GRAFT; SKIN INJURY; STAPHYLOCOCCUS AUREUS; TISSUE ENGINEERING; TISSUE SECTION;

BIOFILMS; DERMOSCOPY; FIBROBLASTS; HUMANS; INFANT, NEWBORN; KERATINOCYTES; PSEUDOMONAS AERUGINOSA; SKIN; SKIN TRANSPLANTATION; STAPHYLOCOCCUS AUREUS; TISSUE ENGINEERING; WOUND HEALING; WOUNDS AND INJURIES;

EID: 69949154805     PISSN: 10760512     EISSN: 15244725     Source Type: Journal    
DOI: 10.1111/j.1524-4725.2009.01238.x     Document Type: Article

References (31)

1. Costerton JW, Stewart PS, Greenberg EP. Bacterial biofilms: a common cause of persistent infections. Science 1999 284 : 1318 1322.
2. Serralta VW, Harrison-Balestra C, Cazzagniga AL, et al. Lifestyles of bacteria in wounds: presence of biofilms? Wounds 2001 13 : 29 34.
3. NIH Guide for Grants and Contracts. (accessed May 15, 2008).
4. Stewart PS, Costerton JW. Antibiotic resistance of bacteria in biofilms. Lancet 2001 358 : 135 138.
5. Moskowitz SM, Foster JM, Emerson J, et al. Clinically feasible biofilm susceptibility assay for isolates of Pseudomonas aeruginosa from patients with cystic fibrosis. J Clin Microbiol 2004 42 : 1915 1922.
6. Saidi IS, Biedlingmaier JF, Whelan P. In vivo resistance to bacterial biofilm formation on tympanostomy tubes as a function of tube material. Otolaryngol Head Neck Surg 1999 120 : 621 627.
7. Dingman JR, Rayner MG, Mishra S, et al. Correlation between presence of viable bacteria and presence of endotoxin in middle-ear effusions. J Clin Microbiol 1998 36 : 3417 3419.
8. Goto T, Nakame Y, Nishida M, Ohi Y. Bacterial biofilms and catheters in experimental urinary tract infection. Int J Antimicrob Agents 1999 11 : 233 236.
9. Tsukamoto T, Matsukawa M, Sano M, et al. Biofilm in complicated urinary tract infection. Int J Antimicrob Agents 1999 11 : 233 236.
10. Stark RM, Gerwig GJ, Pitman RS, et al. Biofilm formation by Helicobacter pylori. Lett Appl Microbiol 1999 28 : 121 126.
11. Akiyama H, Morizane S, Yamasaki O, et al. Assessment of Streptococcus pyogenes microcolony formation in infected skin by confocal laser scanning microscopy. J Dermatol Sci 2003 32 : 193 199.
12. Davis SC, Ricotti C, Cazzaniga AL, Welch E, Mertz PM. Microscopic and physiological evidence for biofilm-associated wound colonization in-vivo. Wound Rep Reg 2008 16 : 23 9.
13. Harrison-Balestra C, Cazzaniga AL, Davis SC. A wound-isolated Pseudomonas aeruginosa grows a biofilm in vitro within 10 hours and is visualized by light microscopy. Dermatol Surg 2003 29 : 631 635.
14. Welsh E, Cazzaniga A, Davis S, Mertz P. Demonstration of Staphylococcus aureus biofilms in acute partial thickness wounds in pigs using electron microscopy. J Invest Dermatol 2003 121 : 619.
15. Bell E, Ehrlich HP, Buttle DJ, Nakatsuji T. Living tissue formed in vitro and accepted as skin-equivalent tissue of full thickness. Science 1981 211 : 1052 1054.
16. Trent JT, Kirsner RS Apligraf: a bi-layered living skin equivalent. Int J Clin Pract 1998 52 : 408 413.
17. Nolte CJ, Oleson MA, Hansbrough JF. Ultrastructural features of composite skin cultures grafted onto athymic mice. J Anat 1994 185 (Pt 2) : 325 333.
18. Parenteau NL, Bilbo P, Nolte CJ, et al. Cytotechnology 1992 9 : 163 171.
19. Organogenesis Inc: http://www.organogenesis.com/content/proddescrp.htm (accessed May 15, 2008).
20. Haldane DJ, Robart E. A comparison of calcofluor white, potassium hydroxide, and culture for the laboratory diagnosis of superficial fungal infection. Diagn Microbiol Infect Dis 1990 13 : 337 339.
21. LePecq JB, Paoletti C. A fluorescent complex between ethidium bromide and nucleic acids. Physical-chemical characterization. J Mol Biol 1967 27 : 87 106.
22. Fux CA, Wilson S, Stoodley P. Detachment characteristics and oxacillin resistance of Staphyloccocus aureus biofilm emboli in an in vitro catheter infection model. J Bacteriol 2004 186 : 4486 4491.
23. Sullivan TP, Eaglstein WH, Davis SC, Mertz P The pig as a model for human wound healing. Wound Repair Regen 2001 9 : 66 76.
24. Davies CE, Hill KE, Wilson MJ, Stephens P. Use of 16S ribosomal DNA PCR and denaturing gradient gel electrophoresis for analysis of the microfloras of healing and nonhealing chronic venous leg ulcers. J Clin Microbiol 2004 42 : 3549 3557.
25. Sibbald RG, Orsted H, Schultz GS. Preparing the wound bed 2003: focus on infection and inflammation. Ostomy Wound Manage 2003 49 : 23S 51S.
26. Browne AC, Vearncombe M. High bacterial load in asymptomatic diabetic patients with neurotrophic ulcers retards wound healing after application of Dermagraft. Ostomy Wound Manage 2001 47 : 44 9.
27. Harriger MD, Warden GD, Greenhalgh DG, et al. Pigmentation and micronanatomy of skin regenerated from composite grafts of cultured cells and biopolymers applied to full-thickness burn wound. Transplantation 1995 59 : 702 707.
28. Supp DM, Karpinski AC, Boyce ST. Expression of human β-defensins HBD-1, HBD-2, and HBD-3 in cultured keratinocytes and skin substitutes. Burns 2004 30 : 643 648.
29. Schmid P, Grenet O, Medina J, et al. An intrinsic antibiotic mechanism in wounds and tissue-engineered skin. J Invest Dermatol 2001 116 : 471 472.
30. Heimbach D, Luterman A, Burke J, et al. Artificial dermis for major burn. A multi-center randomized clinical trial. Ann Surg 1988 208 : 313 320.
31. Maungman P, Deubner H, Honari S, et al. Correlation of clinical outcome of Integra application with microbiologic and pathological biopsies. J Trauma 2006 61 : 1212 1217.