Gene microinjection and integration in C. elegans: Equipments, processes and guidance

Progress in Biochemistry and Biophysics

Volumn 36, Issue 5, 2009, Pages 648-652

  Ya Ming, Jiu ^a   Rong Ying, Zhang ^a   Zheng Xing, Wu ^a  

^a HUAZHONG UNIVERSITY OF SCIENCE AND TECHNOLOGY   (China)

Author keywords

Caenorhabditis elegans; Gene integration; Gene microinjection

Indexed keywords

ANIMALIA; CAENORHABDITIS ELEGANS;

EID: 69949134114     PISSN: 10003282     EISSN: None     Source Type: Journal    
DOI: 10.3724/SP.J.1206.2008.00603     Document Type: Article

References (10)

1. Mello C, Fire A. DNA transformation. Methods Cell Biol, 1995, 48: 451-482
2. Mello C C, Kramer J M, Stinchcomb D, et al. Efficient gene transfer in C. elegans: extrachromosomal maintenance and integration of transforming sequences. EMBO J, 1991, 10(12): 3959-3970
3. Bossinger O, Schierenberg E. Cell-cell communication in the embryo of Caenorhabditis elegans. Dev Biol, 1992, 151(2): 401-409
4. Nott A, Le Hir H, Moore M J. Splicing enhances translation in mammalian cells: an additional function of the exon junction complex. Genes Dev, 2004, 18(2): 210-222
5. Evans T C, Crittenden S L, Kodoyianni V, et al. Translational control of maternal glp-1 mRNA establishes an asymmetry in the C. elegans embryo. Cell, 1994, 77(2): 183-194
6. Maniatis T, Reed R. An extensive network of coupling among gene expression machines. Nature, 2002, 416(6880): 499-506
7. Kimble J, Hodgkin J, Smith T, et al. Suppression of an amber mutation by microinjection of suppressor tRNA in C. elegans. Nature, 1982, 299(5882): 456-458
8. Broverman S, MacMorris M, Blumenthal T. Alteration of Caenorhabditis elegans gene expression by targeted transformation. Proc Natl Acad Sci USA, 1993, 90(10):4359-4363
9. Berezikov E, Bargmann C I, Plasterk R H. Homologous gene targeting in Caenorhabditis elegans by biolistic transformation. Nucleic Acids Res, 2004, 32(4): e40
10. Praitis V, Casey E, Collar D, et al. Creation of low-copy integrated transgenic lines in Caenorhabditis elegans. Genetics, 2001, 157(3): 1217-1226