The efficacy of the fluorescent conjugates of cholera toxin subunit B for multiple retrograde tract tracing in the central nervous system

Brain Structure and Function

Volumn 213, Issue 4-5, 2009, Pages 367-373

  Conte, William L ^a,b   Kamishina, Hiroaki ^c   Reep, Roger L ^a  

^a UNIVERSITY OF FLORIDA   (United States)

^b LOYOLA UNIVERSITY CHICAGO   (United States)

^c IWATE UNIVERSITY   (Japan)

Author keywords

Alexa Fluor; Cholera toxin; Fluorescence; Neuroanatomy; Retrograde tracer; Tract tracing

Indexed keywords

3K DEXTRAN AMINE; ALEXA FLUOR 488 CHOLERA TOXIN B SUBUNIT CONJUGATE; ALEXA FLUOR 594 CHOLERA TOXIN B SUBUNIT CONJUGATE; AMINE; CHOLERA TOXIN B SUBUNIT; DEXTRAN DERIVATIVE; TRACER; UNCLASSIFIED DRUG;

ANIMAL EXPERIMENT; ANIMAL TISSUE; ARTICLE; CELL LABELING; CENTRAL NERVOUS SYSTEM; CINGULATE GYRUS; CONFOCAL MICROSCOPY; CONTROLLED STUDY; FLUORESCENCE; INJECTION SITE; MALE; MICROSCOPY; NEUROANATOMY; NONHUMAN; PRIORITY JOURNAL; RAT; THALAMUS POSTERIOR NUCLEUS; VISCOSITY;

ANIMALS; CHOLERA TOXIN; FLUORESCENT DYES; GYRUS CINGULI; MALE; MICROSCOPY, CONFOCAL; MOTOR CORTEX; NEURAL PATHWAYS; POSTERIOR THALAMIC NUCLEI; RATS; RATS, LONG-EVANS; STAINING AND LABELING;

ALEXA; RATTUS;

EID: 69949118055     PISSN: 18632653     EISSN: 18632661     Source Type: Journal    
DOI: 10.1007/s00429-009-0212-x     Document Type: Article

References (26)

1. JE Berlier A Rothe G Buller J Bradford DR Gray BJ Filanoski WG Telford S Yue J Liu C-Y Cheung W Chang JD Hirsch JM Beechem RP Haugland RP Haugland 2003 Quantitative comparison of long-wavelength Alexa Fluor dyes to Cy dyes: fluorescence of the dyes and their bioconjugates J Histochem Cytochem 51 1699 1712
2. S Chen G Aston-Jones 1995 Evidence that cholera toxin b subunit (CTB) can be avidly taken up and transported by fibers of passage Brain Res 674 107 111
3. JA Christianson R Liang EE Ustinova BM Davis MO Fraser MA Pezzone 2007 Convergence of bladder and colon sensory innervation occurs at the primary afferent level Pain 128 235 243
4. WL Conte H Kamishina JV Corwin RL Reep 2008 Topography in the projections of lateral posterior thalamus with cingulate and medial agranular cortex in relation to circuitry for directed attention and neglect Brain Res 1240 87 95
5. WL Conte H Kamishina RL Reep 2009 Multiple neuroanatomical tract-tracing using fluorescent Alexa Fluor conjugates of cholera toxin subunit b in rats Nat Protoc 4 1157 1166
6. LM Coolen HT Jansen RL Goodman RI Wood MN Lehman 1999 A new method for simultaneous demonstration of anterograde and retrograde connections in the brain: co-injections of biotinylated dextran amine and the beta subunit of cholera toxin J Neurosci Methods 91 1 8
7. P Dederen A Gribnau M Curfs 1994 Retrograde neuronal tracing with cholera toxin b subunit: comparison of three different visualization methods Histochem J 26 856 862
8. GH DeVries WJ Zetusky C Zmachinski VP Calabrese 1981 Lipid composition of axolemma-enriched fractions from human brains J Lipid Res 22 208 216
9. PH Fishman 1982 Role of membrane gangliosides in the binding and action of bacterial toxins J Membr Biol 69 85 97
10. H Kamishina GH Yurcisin JV Corwin RL Reep 2008 Striatal projections from the rat lateral posterior thalamic nucleus Brain Res 1204 24 39
11. H Kamishina WL Conte SS Patel RJ Tai JV Corwin RL Reep 2009 Cortical connections of the rat lateral posterior thalamic nucleus Brain Res 1264 39 56
12. C Kobbert R Apps I Bechmann JL Lanciego J Mey S Thanos 2000 Current concepts in neuroanatomical tracing Prog Neurobiol 62 327 351
13. F Kreier YS Kap TC Mettenleiter C van Heijningen J van der Vliet A Kalsbeek HP Sauerwein E Fliers JA Romijn RM Buijs 2006 Tracing from fat tissue, liver, and pancreas: a neuroanatomical framework for the role of the brain in type 2 diabetes Endocrinology 147 1140 1147
14. KE Krout RE Belzer AD Loewy 2002 Brainstem projections to midline and intralaminar thalamic nuclei of the rat J Comp Neurol 448 53 101
15. RK Kumar CC Chapple N Hunter 1999 Improved double immunofluorescence for confocal laser scanning microscopy J Histochem Cytochem 47 1213 1218
16. IJ Llewellyn-Smith CL Martin LF Arnolda JB Minson 2000 Tracer-toxins: cholera toxin b-saporin as a model J Neurosci Methods 103 83 90
17. P-H Luppi P Fort M Jouvet 1990 Iontophoretic application of unconjugated cholera toxin b subunit (CTB) combined with immunohistochemistry of neurochemical substances: a method for transmitter identification of retrogradely labeled neurons Brain Res 534 209 224
18. D Masco M Van de Walle S Spiegel 1991 Interaction of ganglioside GM1 with the B subunit of cholera toxin modulates growth and differentiation of neuroblastoma N18 cells J Neurosci 11 2443 2452
19. S McDavid JP Lund F Auclair A Kolta 2006 Morphological and immunohistochemical characterization of interneurons within the rat trigeminal motor nucleus Neuroscience 139 1049 1059
20. L Muscat AD Huberman CL Jordan LP Morin 2003 Crossed and uncrossed retinal projections to the hamster circadian system J Comp Neurol 466 513 524
21. M Niedringhaus PG Jackson R Pearson M Shi K Dretchen RA Gillis N Sahibzada 2008 Brainstem sites controlling the lower esophageal sphincter and crural diaphragm in the ferret: a neuroanatomical study Auton Neurosci 144 50 60
22. N Panchuk-Voloshina RP Haugland J Bishop-Stewart MK Bhalgat PJ Millard F Mao W-Y Leung RP Haugland 1999 Alexa dyes, a series of new fluorescent dyes that yield exceptionally bright, photostable conjugates J Histochem Cytochem 47 1179 1188
23. Paxinos G, Watson C (2005) The rat brain in stereotaxic coordinates, 5th edn. Elsevier Academic Press, San Diego
24. WE van Heyningen 1974 Gangliosides as membrane receptors for tetanus toxin, cholera toxin and serotonin Nature 249 415 417
25. HF Wang P Shortland MJ Park G Grant 1998 Retrograde and transganglionic transport of horseradish peroxidase-conjugated cholera toxin b subunit, wheatgerm agglutinin and isolectin b4 from griffonia simplicifolia i in primary afferent neurons innervating the rat urinary bladder Neuroscience 87 275 288
26. S Zin-Ka-Ieu M Roger P Arnault 1997 Direct contacts between fibers from the ventrolateral thalamic nucleus and frontal cortical neurons projecting to the striatum: a light-microscopy study in the rat Anat Embryol 197 77 87