Synthesis of 13C7-labeled iodoacetanilide and application to quantitative analysis of peptides and a protein by isotope differential mass spectrometry

Bioorganic and Medicinal Chemistry Letters

Volumn 19, Issue 19, 2009, Pages 5698-5702

  Niwayama, Satomi ^a   Zabet Moghaddam, Masoud ^a   Kurono, Sadamu ^b,c   Cho, Hanjoung ^a  

^a TEXAS TECH UNIVERSITY   (United States)

^b OSAKA UNIVERSITY GRADUATE SCHOOL OF MEDICINE   (Japan)

^c FUJIFILM Wako Pure Chemical Corporation   (Japan)

Author keywords

Cysteine modifier; Mass spectrometry; Proteomics; Quantitative analysis of peptides

Indexed keywords

CYSTEINE; IODOACETANILIDE 13 C; ISOTOPE; UNCLASSIFIED DRUG; ACETANILIDE DERIVATIVE; BOVINE SERUM ALBUMIN; CARBON; IODOACETANILIDE; PEPTIDE; PROTEIN;

AMINO ACID SEQUENCE; ARTICLE; COLLISIONALLY ACTIVATED DISSOCIATION; MASS SPECTROMETRY; MATRIX ASSISTED LASER DESORPTION IONIZATION TIME OF FLIGHT MASS SPECTROMETRY; MOLECULAR WEIGHT; PEPTIDE ANALYSIS; PROTEIN ANALYSIS; QUANTITATIVE ANALYSIS; SYNTHESIS; ANIMAL; CATTLE; CHEMISTRY; ELECTROSPRAY MASS SPECTROMETRY; ISOTOPE LABELING; METHODOLOGY;

ACETANILIDES; ANIMALS; CARBON ISOTOPES; CATTLE; ISOTOPE LABELING; PEPTIDES; PROTEINS; SERUM ALBUMIN, BOVINE; SPECTROMETRY, MASS, ELECTROSPRAY IONIZATION;

EID: 69949083582     PISSN: 0960894X     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.bmcl.2009.08.011     Document Type: Article

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44. 7-IAA modified peptides were used for calculation of the relative ratios. The averages of these data were plotted on the graph.
45. 9 The extracted peptide solution was dried by Speed Vacuum and kept at -20 °C for further analysis by the MALDI mass spectrometer.The dried samples were mixed with 10 μL of the solution containing 50% acetonitrile and 0.1% TFA. The digested sample (0.5 μL) was deposited on a MALDI-plate followed by the addition of 0.5 μL of a matrix consisting of 5 mg/mL of α-cyano-4-hydroxycinnamic acid (CHCA) in 50% acetonitrile and 0.1% trifluoroacetic acid (TFA).
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