Construction, fermentation and purification of high polymer spider dragline silk protein containing RGD peptide

Shengwu Gongcheng Xuebao/Chinese Journal of Biotechnology

Volumn 23, Issue 5, 2007, Pages 858-861

  Ruan, Chao Ran ^a   Huang, Jing Xing ^a   Wei, Mei Hong ^a   Li, Min ^a  

^a FUJIAN NORMAL UNIVERSITY   (China)

Author keywords

High cell density fermentation; Purification; RGD peptide; Silk protein

Indexed keywords

ARANEAE; PROKARYOTA;

ARGINYL-GLYCYL-ASPARTIC ACID; ARGINYLGLYCYLASPARTIC ACID; FIBROIN; OLIGOPEPTIDE; POLYMER; RECOMBINANT PROTEIN; SPIDROIN 2;

ANIMAL; ARTICLE; BACTERIUM TRANSFORMATION; BIOSYNTHESIS; CHEMISTRY; ESCHERICHIA COLI; FERMENTATION; GENETICS; ISOLATION AND PURIFICATION; METABOLISM; MOLECULAR GENETICS; PROTEIN ENGINEERING;

ANIMALS; ESCHERICHIA COLI; FERMENTATION; FIBROINS; MOLECULAR SEQUENCE DATA; OLIGOPEPTIDES; POLYMERS; PROTEIN ENGINEERING; RECOMBINANT PROTEINS; TRANSFORMATION, BACTERIAL;

EID: 69849107214     PISSN: 10003061     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (9)

1. Vollrath F, Knight DP. Liquid crystalline spinning of spider silk. Nature, 2001, 410:541-548.
2. Tirrell DA. Putting a new spin on spider silk. Science, 1996, 271: 39-40.
3. Li M , Huang JK , Tu GY , et al. Biological synthesis and purification of spider dragline silk protein polymers containing RGD three peptide. Journal of Biomedical Engineering 2004, 21(6) : 1006-1010.
4. Li M HuangZH, et al. Study on the-conditions of high density fermentation for the engineering. Journal of Biomedical Engineering, 2005, 22(6), 1206-1209.
5. Riekel C, Vollrath F. Spider silk fibre extrusion: combined wide- and small-angle X-ray microdiffraction experiments. International Journal of Biological Macromolecules, 2001,29:203-210.
6. Li MZ, Lu SZ, Wu ZY, et al. Structure and properties of silk fibroin-poly (vinyl alcohol) gel. International Journal of Biological Macromolecules, 2002, 30 : 89-94.
7. Hinman MB, Lewis RV. Isolation of a clone ecoding a second dragline Silk fibroin. J Biol Chem, 1992, 267(27): 19320.
8. Xu M, Lewis RV. Structure of a protein superfiber: spider dragline silk. Proc Natl Acad Sci, 1990, 87(18) :7120.
9. Li M(, Huang ZH, et al. Study on construct and expression of synthetic genes encoding spider dragline silk in Escherichia coli. Chinese Journal of Biotechnology , 2002, 18(3) :331-334.