A solid-phase adsorption method for PEGylation of human serum albumin and staphylokinase: Preparation, purification and biochemical characterization

Biotechnology Letters

Volumn 31, Issue 8, 2009, Pages 1191-1196

  Suo, Xiaoyan ^a,b   Lu, Xiuling ^a   Hu, Tao ^a   Ma, Guanghui ^a   Su, Zhiguo ^a  

^a INSTITUTE OF PROCESS ENGINEERING   (China)

^b UNIVERSITY OF CHINESE ACADEMY OF SCIENCES   (China)

Author keywords

Human serum albumin; PEGylation; Solid phase; Staphylokinase

Indexed keywords

BIOCHEMICAL CHARACTERIZATION; BIOLOGICAL ACTIVITIES; CIRCULAR DICHROISM; CONFORMATIONAL CHANGE; HUMAN SERUM ALBUMIN; HUMAN SERUM ALBUMINS; INTRINSIC FLUORESCENCE; ION EXCHANGE CHROMATOGRAPHY; LIQUID PHASE; MODEL PROTEINS; PEGYLATION; POSITIVE CHARGES; Q-SEPHAROSE; SDS-PAGE; SOLID-PHASE; STAPHYLOKINASE;

ADSORPTION; BODY FLUIDS; CHROMATOGRAPHIC ANALYSIS; DICHROISM; DIFFUSERS (OPTICAL); GEL PERMEATION CHROMATOGRAPHY; ION CHROMATOGRAPHY; ION EXCHANGE; LIQUIDS; PROTEINS;

SIZE EXCLUSION CHROMATOGRAPHY;

ALB PROTEIN, HUMAN; AUR PROTEIN, STAPHYLOCOCCUS AUREUS; GLYCOPROTEIN; MACROGOL DERIVATIVE; METALLOPROTEINASE; SERUM ALBUMIN;

ADSORPTION; ARTICLE; CHEMISTRY; CIRCULAR DICHROISM; FLUOROMETRY; HUMAN; METABOLISM; METHODOLOGY; PROTEIN BINDING;

ADSORPTION; CIRCULAR DICHROISM; FLUOROMETRY; GLYCOPROTEINS; HUMANS; METALLOENDOPEPTIDASES; POLYETHYLENE GLYCOLS; PROTEIN BINDING; SERUM ALBUMIN;

EID: 69849099305     PISSN: 01415492     EISSN: 15736776     Source Type: Journal    
DOI: 10.1007/s10529-009-9986-4     Document Type: Article

References (13)

1. Chang TMS (1999) Future prospects for artificial blood. Trends Biotechnol 181:61-67.
2. Felix AM (1997) Site-specific poly(ethylene glycol)ylation of peptides. In: Harris JM, Zalipsky S (eds) Poly(ethylene glycol): chemistry and biological applications. American Chemical Society, Washington DC, pp 218-238.
3. Harris JM, Zalipsky S (1997) Poly (ethylene glycol) chemistry and biological applications. American Chemical Society, Washington DC.
4. Hauptmann J, Guhrs KH, Hartmann M, Schlott B (1995) The fibrinolytic activity of staphylokinase mutants in the fibrin plate assay. Haemostasis 25:272-276.
5. Hu T, Su ZG (2003) A solid phase adsorption method for preparation of bovine serum albumin-bovine hemoglobin conjugate. J Biotechnol 100:267-275.
6. Hu T, Li D, Su ZG (2003) Preparation and characterization of dimeric bovine hemoglobin tetramers. J Protein Chem 22:411-416.
7. Laemmli UK (1970) Cleavage of structural protein during the assembly of the head of bacteriophage T4. Nature 227:680-685.
8. Lee BK, Kwon JS, Kim HJ, Yamamoto S, Lee EK (2007) Solid-phase PEGylation of recombinant interferon α-2a for site-specific modification: process performance, characterization, and in vitro bioactivity. Bioconjugate Chem 18:1728-1734.
9. Manjula BN, Tsai A (2003) Site-specific PEGylation of hemoglobin at Cys-93(β): correlation between the colligative properties of the PEGylated protein and the length of the conjugated PEG chain. Bioconjugate Chem 14:464-472.
10. Planas M, Cros E, Rodrguez RA, Ferre R, Bardaj E (2002) Solid-phase synthesis of new peptide-arene hybrids from N-TCP amino acids. Tetrahedron Lett 43:4431-4434.
11. Rajamohan G, Dikshit KD (2000) Role of the N-terminal region of staphylokinase (SAK): evidence for the participation of the N-terminal region of SAK in the enzyme-substrate complex formation. FEBS Lett 474:151-158.
12. Sako T, Sawaki S, Sakurai T, Ito S, Yoshizawa Y, Kondo I (1983) Cloning and expression of the staphylokinase gene of Staphylococcus aureus. Mol Gen Genet 190:271-277.
13. Zalipsky S, Seltzar R, Menon RS (1992) Evaluation of a new reagent for covalent attachment of polyethylene glycol to proteins. Biotechnol Appl Bioc 15:100-114.