Rapid detection and quantification of zearalenone-producing Fusarium species by targeting the zearalenone synthase gene PKS4

Food Control

Volumn 21, Issue 2, 2010, Pages 207-211

  Meng, Kun ^a   Wang, Yaru ^a   Yang, Peilong ^a   Luo, Huiying ^a   Bai, Yingguo ^a   Shi, Pengjun ^a   Yuan, Tiezheng ^a   Ma, Rui ^a   Yao, Bin ^a  

^a AGRO ENVIRONMENTAL PROTECTION INSTITUTE   (China)

Author keywords

Fusarium; Real time PCR; SYBR Green I; Zearalenone

Indexed keywords

FUSARIUM; ZEA MAYS;

EID: 69649086908     PISSN: 09567135     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.foodcont.2009.05.014     Document Type: Article

References (16)

1. Burgess L.W., Nelson P.E., and Toussoun T.A. Characterization, geographic distribution and ecology of Fusarium crookwellense sp. nov. Transactions of the British Mycological Society 79 (1982) 497-505
2. Dean T.R., Betancourt D., and Menetrez M.Y. A rapid DNA extraction method for PCR identification of fungal indoor air contaminants. Journal of Microbiological Methods 56 (2004) 431-434
3. Dean T.R., Roop B., Betancourt D., and Menetrez M.Y. A simple multiplex polymerase chain reaction assay for the identification of four environmentally relevant fungal contaminants. Journal of Microbiological Methods 61 (2005) 9-16
4. El-Kady I.A., and El-Maraghy S.S. Screening of zearalenone-producing Fusarium species in Egypt and chemically defined medium for production of the toxin. Mycopathologia 78 (1982) 25-29
5. Gaffoor I., and Trail F. Characterization of two polyketide synthase genes involved in zearalenone biosynthesis in Gibberella zeae. Applied and Environmental Microbiology 72 (2006) 1793-1799
6. Hacking A., Rosser W.R., and Dervish M.T. Zearalenone-producing species of Fusarium on barley seed. Annals of Applied Biology 84 (1976) 7-11
7. Labuda R., Parich A., Berthiller F., and Tancinová D. Incidence of trichothecenes and zearalenone in poultry feed mixtures from Slovakia. International Journal of Food Microbiology 105 (2005) 19-25
8. Lea P., and Christoph V.H. Determination of zearalenone from wheat and corn by pressurized liquid extraction and liquid chromatography-electrospray mass spectrometry. Journal of Chromatography A 993 (2003) 39-45
9. Lysøe E., Klemsdal S.S., Bone K.R., Frandsen R.J.N., Johansen T., Thrane U., et al. The PKS4 gene of Fusarium graminearum is essential for zearalenone production. Applied and Environmental Microbiology 72 (2006) 3924-3932
10. Mayer Z., Bagnara A., Farber P., and Geisen R. Quantification of the copy number of nor-1, a gene of the aflatoxin biosynthetic pathway by real-time PCR, and its correlation to the cfu of Aspergillus flavus in foods. International Journal of Food Microbiology 82 (2003) 143-151
11. Niessen L. PCR-based diagnosis and quantification of mycotoxin producing fungi. International Journal of Food Microbiology 119 (2007) 38-46
12. Sambrook J., Fritsch E.F., and Maniatis T. Molecular cloning: A laboratory manual. 2nd ed. (1989), Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York
13. Songsermsakul P., Sontag G., Cichna-Markl M., Zentek J., and Razzazi E. Determination of zearalenone and its metabolites in urine, plasma and faeces of horses by HPLC-APCI-MS. Journal of Chromatography B 843 (2006) 252-261
14. Steven D.H. Morphogenesis in germinating Fusarium graminearum macroconidia. Mycologia 97 (2005) 880-887
15. Sugiura Y., Saito H., Tanaka T., Ichinoe M., and Ueno Y. Fusarium crookwellense, a newly isolated fungus from wheat in Japan: Its mycotoxin production and pathogenicity to wheat and barley. Mycoscience 35 (1994) 77-82
16. Toppari J., Larsen J.C., Christiansen P., Giwercman A., Grandjean P., Guillette L.J., et al. Male reproductive health and environmental xenoestrogens. Environmental Health Perspectives 104 (1996) 741-803