Stable reversal of multidrug resistance in colon cancer cells by RNA interference targeting the MDR1 gene

Molecular Medicine Reports

Volumn 2, Issue 4, 2009, Pages 579-584

  Xia, Zhongsheng ^a   Zhang, Liyong ^b   Chen, Qikui ^a   Royal, Crista ^b   Yu, Zhong ^a   Liu, Zhongmin ^b   Adam, Bao Ling ^b  

^a SUN YAT SEN UNIVERSITY   (China)

^b MEDICAL COLLEGE OF GEORGIA   (United States)

Author keywords

Colon cancer; MDR1; Multidrug resistance; P glycoprotein; RNA interference; Stable transfection

Indexed keywords

DOXORUBICIN; MULTIDRUG RESISTANCE PROTEIN 1; PLASMID VECTOR; SMALL INTERFERING RNA; VINCRISTINE;

ARTICLE; CANCER CELL; CANCER CELL CULTURE; CANCER RESISTANCE; CELL CLONE; CELL CYCLE; CELL VIABILITY; COLON CANCER; CONTROLLED STUDY; DRUG ACCUMULATION; FLOW CYTOMETRY; GENE CONSTRUCT; GENE IDENTIFICATION; GENE OVEREXPRESSION; GENE TARGETING; GENETIC SCREENING; GENETIC TRANSFECTION; HUMAN; HUMAN CELL; HUMAN CELL CULTURE; IC 50; MULTIDRUG RESISTANCE; NUCLEOTIDE SEQUENCE; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; REVERTANT; RNA INTERFERENCE; STABLE TRANSFECTION; WESTERN BLOTTING;

EID: 69549118652     PISSN: 17912997     EISSN: 17913004     Source Type: Journal    
DOI: 10.3892/mmr_00000140     Document Type: Article

References (20)

1. Hawk ET, Limburg PJ and Viner JL: Epidemiology and prevention of colorectal cancer. Surg Clin North Am 82: 905-941, 2002.
2. Taa BG, Van Tinteren H and Zoetmulder FA: Adjuvant 5-FU plus levamisole in colonic or rectal cancer: improved survival in stage II and III. Br J Cancer 85: 1437-1443, 2001. (Pubitemid 34001570)
3. Redmond SM, Joncourt F, Buser K, et al: Assessment of P-glycoprotein, glutathione-based detoxifying enzymes and O6-alkyl-guanine-DNA alkyltransferase as potential indicators of constitutive drug resistance in human colorectal tumors. Cancer Res 51: 2092-2097, 2001.
4. Germann UA, Pastan I and Gottesman MM: P-glycoproteins: mediators of multidrug resistance. Semin Cell Biol 4: 63-76, 1993.
5. Lage H: ABC-transporters: implication on drug resistance from microorganisms to human cancers. Int J Antimicrob Agents 22: 188-199, 2003.
6. Van de Vrie W, Gheuens EE, Durante NM, et al: In vitro and in vivo chemosensitizing effect of cyclosporin A on an intrinsic multidrug-resistant rat colon tumour. J Cancer Res Clin Oncol 119: 609-614, 1993.
7. Quattrone A, Papucci L, Morganti M, et al: Inhibition of MDR1 gene expression by antimessenger oligonucleotides lowers multiple drug resistance. Oncol Res 6: 311-320, 1994 (Pubitemid 24370314)
8. Kobayashi H, Dorai T, Holland JF, et al: Reversal of drug sensitivity in multidrug-resistant tumor cells by an MDR1 (PGY1) ribozyme. Cancer Res 54: 1271-1275, 1994 (Pubitemid 24108068)
9. Damiani D, Michieli M, Michelutti A, et al: D-verapamil downmodulates P170-associated resistance to doxorubicin, daunorubicin and idarubicin. Anticancer Drugs 4: 173-180, 1993. (Pubitemid 23115130)
10. Xia Z, Zhu Z, Zhang L, et al: Specific reversal of MDR1/P-gp-dependent multidrug resistance by RNA interference in colon cancer cells. Oncol Rep 20: 1433-1439, 2008.
11. Carmichael J, Degraff WG, Gazdar AF, et al: Evaluation of a tetrazolium-based semiautomated colorimetric assay: assessment of chemosensitivity testing. Cancer Res 47: 936-942, 1987. (Pubitemid 17027905)
12. Bai M, Agnantis NJ, Kamina S, et al: In vivo cell kinetics in breast carcinogenesis. Breast Cancer Res 3: 276-283, 2001.
13. Li J, Xu LZ, He KL, et al: Reversal effects of nomegestrol acetate on multidrug resistance in adriamycin-resistant MCF7 breast cancer cell line. Breast Cancer Res 3: 253-263, 2001 (Pubitemid 32633348)
14. Van Rensburg CE, Joone GK and O'Sullivan JF: Clofazimine and B4121 sensitize an intrinsically resistant human colon cancer cell line to P-glycoprotein substrates. Oncol Rep 7: 193-195, 2000.
15. Kok JW, Veldman RJ, Klappe K, et al: Differential expression of sphingolipids in MRP1 overexpressing HT29 cells. Int J Cancer 87: 172-178, 2000. (Pubitemid 30434341)
16. Celius T, Garberg P and Lundgren B: Stable suppression of MDR1 gene expression and function by RNAi in Caco-2 cells. Biochem Biophys Res Commun 324: 365-371, 2004. (Pubitemid 39311504)
17. Nieth C, Priebsch A, Stege A, et al: Modulation of the classical multidrug resistance (MDR) phenotype by RNA interference (RNAi). FEBS Lett 545: 144-150, 2003.
18. Licht T, Pastan I, Gottesman M, et al: P-glycoprotein-mediated multidrug resistance in normal and neoplastic hematopoietic cells. Ann Hematol 69: 159-171, 1994 (Pubitemid 24314384)
19. Smeets M, Raymakers R, Vierwinden G, et al: A low but functionally significant MDR1 expression protects primitive haemopoietic progenitor cells from anthracycline toxicity. Br J Haematol 96: 346-355, 1997. (Pubitemid 27077371)
20. Holm PS, Lage H, Bergmann S, et al: Multidrug-resistant cancer cells facilitate E1-independent adenoviral replication: impact for cancer gene therapy. Cancer Res 64: 322-328, 2004. (Pubitemid 38114114)