New design strategy for development of specific primer sets for PCR-based detection of Chlorophyceae and Bacillariophyceae in environmental samples

Applied and Environmental Microbiology

Volumn 75, Issue 17, 2009, Pages 5729-5733

  Moro, Claire Valiente ^a,b   Crouzet, Olivier ^a   Rasconi, Séréna ^a   Thouvenot, Antoine ^a   Coffe, Gérard ^a   Batisson, Isabelle ^a   Bohatier, Jacques ^a  

^a UNIVERSITÉ BLAISE PASCAL   (France)

^b UNIVERSITÉ CLERMONT AUVERGNE   (France)

Author keywords

[No Author keywords available]

Indexed keywords

18S RRNA; AQUATIC ENVIRONMENTS; BACILLARIOPHYCEAE; BIO-INDICATORS; ENVIRONMENTAL SAMPLE; HEALTH STATUS; MICRO-ALGAE; NEW DESIGN; NON-TARGETED; PCR PRIMER SETS; PCR PRIMERS; PCR-BASED DETECTION; RRNA GENES; SPECIFIC PRIMERS;

AIR POLLUTION CONTROL; BIODIVERSITY; WATER POLLUTION; WATER QUALITY;

STRATEGIC PLANNING;

BIOLOGICAL MARKER; FRESH WATER; RNA 18S;

BIODIVERSITY; BIOINDICATOR; DETECTION METHOD; FRESHWATER ECOSYSTEM; MICROALGA; MICROBIAL ECOLOGY; NONTARGET ORGANISM; POLYMERASE CHAIN REACTION; RNA; SOFTWARE; WATER QUALITY;

ACCURACY; ALGA; AQUATIC ENVIRONMENT; ARTICLE; BACILLARIOPHYCEAE; BACTERIUM DETECTION; CHLOROPHYCEAE; CILIATE; COMPUTER PROGRAM; CONTROLLED STUDY; ECOLOGY; FUNGUS; HEALTH STATUS; NONHUMAN; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION; SPECIES IDENTIFICATION;

ALGAE, GREEN; COMPUTATIONAL BIOLOGY; COMPUTER SIMULATION; DIATOMS; DNA PRIMERS; DNA, ALGAL; DNA, RIBOSOMAL; ENVIRONMENTAL MICROBIOLOGY; MOLECULAR SEQUENCE DATA; PHYLOGENY; POLYMERASE CHAIN REACTION; RNA, ALGAL; RNA, RIBOSOMAL, 18S; SENSITIVITY AND SPECIFICITY; SEQUENCE ANALYSIS, DNA; SEQUENCE HOMOLOGY;

ALGAE; BACILLARIOPHYCEAE; CHLOROPHYCEAE; CILIOPHORA; FUNGI;

EID: 69449084551     PISSN: 00992240     EISSN: 10985336     Source Type: Journal    
DOI: 10.1128/AEM.00509-09     Document Type: Article

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