Single-molecule microscopy reveals membrane microdomain organization of cells in a living vertebrate

Biophysical Journal

Volumn 97, Issue 4, 2009, Pages 1206-1214

  Schaaf, Marcel J M ^a   Koopmans, Wiepke J A ^b   Meckel, Tobias ^b   Van Noort, John ^b   Snaar Jagalska, B Ewa ^a   Schmidt, Thomas S ^b   Spaink, Herman P ^a  

^a LEIDEN UNIVERSITY   (Netherlands)

^b LEIDEN UNIVERSITY   (Netherlands)

Author keywords

[No Author keywords available]

Indexed keywords

MEMBRANE PROTEIN; YELLOW FLUORESCENT PROTEIN;

ARTICLE; CELL MEMBRANE; EMBRYONIC STEM CELL; EPIDERMIS CELL; FLUORESCENCE MICROSCOPY; MEMBRANE STRUCTURE; MICROSCOPY; NONHUMAN; SINGLE MOLECULE MICROSCOPY; ZEBRA FISH;

DANIO RERIO; VERTEBRATA;

EID: 69349090833     PISSN: 00063495     EISSN: 15420086     Source Type: Journal    
DOI: 10.1016/j.bpj.2009.05.044     Document Type: Article

References (37)

1. Kusumi, A., H. Ike, C. Nakada, K. Murase, and T. Fujiwara. 2005. Single-molecule tracking of membrane molecules: plasma membrane compartmentalization and dynamic assembly of raft-philic signaling molecules. Semin. Immunol. 17:3-21.
2. Schmidt, T., G. J. Schutz, W. Baumgartner, H. J. Gruber, and H. Schindler. 1996. Imaging of single molecule diffusion. Proc. Natl. Acad. Sci. USA. 93:2926-2929.
3. Sako, Y., S. Minoghchi, and T. Yanagida. 2000. Single-molecule imaging of EGFR signalling on the surface of living cells. Nat. Cell Biol. 2:168-172.
4. Schutz, G. J., G. Kada, V. P. Pastushenko, and H. Schindler. 2000. Properties of lipid microdomains in a muscle cell membrane visualized by single molecule microscopy. EMBO J. 19:892-901.
5. Harms, G. S., L. Cognet, P. H. Lommerse, G. A. Blab, and T. Schmidt. 2001. Autofluorescent proteins in single-molecule research: applications to live cell imaging microscopy. Biophys. J. 80:2396-2408.
6. de Keijzer, S., A. Serge, F. van Hemert, P. H. Lommerse, G. E. Lamers, et al. 2008. A spatially restricted increase in receptor mobility is involved in directional sensing during Dictyostelium discoideum chemotaxis. J. Cell Sci. 121:1750-1757. (Pubitemid 351911831)
7. Harms, G. S., M. Sonnleitner, G. J. Schutz, H. J. Gruber, and T. Schmidt. 1999. Single-molecule anisotropy imaging. Biophys. J. 77:2864-2870.
8. Iino, R., I. Koyama, and A. Kusumi. 2001. Single molecule imaging of green fluorescent proteins in living cells: E-cadherin forms oligomers on the free cell surface. Biophys. J. 80:2667-2677. (Pubitemid 32521642)
9. Ike, H., A. Kosugi, A. Kato, R. Iino, H. Hirano, et al. 2003. Mechanism of Lck recruitment to the T-cell receptor cluster as studied by single-molecule-fluorescence video imaging. ChemPhysChem. 4:620-626.
10. Lommerse, P. H., B. E. Snaar-Jagalska, H. P. Spaink, and T. Schmidt. 2005. Single-molecule diffusion measurements of H-Ras at the plasma membrane of live cells reveal microdomain localization upon activation. J. Cell Sci. 118:1799-1809.
11. Blaser, H., S. Eisenbeiss, M. Neumann, M. Reichman-Fried, B. Thisse, et al. 2005. Transition from non-motile behaviour to directed migration during early PGC development in zebrafish. J. Cell Sci. 118:4027-4038. (Pubitemid 41410284)
12. Pauls, S., B. Geldmacher-Voss, and J. A. Campos-Ortega. 2001. A zebrafish histone variant H2A.F/Z and a transgenic H2A.F/Z:GFP fusion protein for in vivo studies of embryonic development. Dev. Genes Evol. 211:603-610.
13. Niell, C. M., M. P. Meyer, and S. J. Smith. 2004. In vivo imaging of synapse formation on a growing dendritic arbor. Nat. Neurosci. 7:254-260.
14. Malumbres, M., and M. Barbacid. 2003. RAS oncogenes: the first 30 years. Nat. Rev. Cancer. 3:459-465.
15. Lommerse, P. H., G. A. Blab, L. Cognet, G. S. Harms, B. E. Snaar-Jagalska, et al. 2004. Single-molecule imaging of the H-ras membrane-anchor reveals domains in the cytoplasmic leaflet of the cell membrane. Biophys. J. 86:609-616.
16. Lommerse, P. H., K. Vastenhoud, N. J. Pirinen, A. I. Magee, H. P. Spaink, et al. 2006. Single-molecule diffusion reveals similar mobility for the Lck, H-ras, and K-ras membrane anchors. Biophys. J. 91:1090-1097.
17. He, S., E. Salas-Vidal, S. Rueb, S. F. Krens, A. H. Meijer, et al. 2006. Genetic and transcriptome characterization of model zebrafish cell lines. Zebrafish. 3:441-453. (Pubitemid 46089241)
18. Koopmans, W. J., A. Brehm, C. Logie, T. Schmidt, and J. van Noort. 2007. Single-pair FRET microscopy reveals mononucleosome dynamics. J. Fluoresc. 17:785-795. (Pubitemid 350075697)
19. Schutz, G. J., H. Schindler, and T. Schmidt. 1997. Single-molecule microscopy on model membranes reveals anomalous diffusion. Biophys. J. 73:1073-1080.
20. Bobroff, N. 1986. Position measurement with a resolution and noise-limited instrument. Rev. Sci. Instrum. 57:1152-1157.
21. Anderson, C. M., G. N. Georgiou, I. E. Morrison, G. V. Stevenson, and R. J. Cherry. 1992. Tracking of cell surface receptors by fluorescence digital imaging microscopy using a charge-coupled device camera. Low-density lipoprotein and influenza virus receptor mobility at 4°C. J. Cell Sci. 101:415-425.
22. Qian, H., M. P. Sheetz, and E. L. Elson. 1991. Single particle tracking. Analysis of diffusion and flow in two-dimensional systems. Biophys. J. 60:910-921.
23. Kusumi, A., Y. Sako, and M. Yamamoto. 1993. Confined lateral diffusion of membrane receptors as studied by single particle tracking (nanovid microscopy). Effects of calcium-induced differentiation in cultured epithelial cells. Biophys. J. 65:2021-2040.
24. Wieser, S., and G. J. Schutz. 2008. Tracking single molecules in the live cell plasma membrane: do's and don't's. Methods. 46:131-140.
25. Driever, W., and Z. Rangini. 1993. Characterization of a cell line derived from zebrafish (Brachydanio rerio) embryos. In Vitro Cell. Dev. Biol. Anim. 29A:749-754. (Pubitemid 23315948)
26. Montero, J. A., B. Kilian, J. Chan, P. E. Bayliss, and C. P. Heisenberg. 2003. Phosphoinositide 3-kinase is required for process outgrowth and cell polarization of gastrulating mesendodermal cells. Curr. Biol. 13:1279-1289.
27. Kimmel, C. B., W. W. Ballard, S. R. Kimmel, B. Ullmann, and T. F. Schilling. 1995. Stages of embryonic development of the zebrafish. Dev. Dyn. 203:253-310.
28. Le Guellec, D., G. Morvan-Dubois, and J. Y. Sire. 2004. Skin development in bony fish with particular emphasis on collagen deposition in the dermis of the zebrafish (Danio rerio). Int. J. Dev. Biol. 48:217-231.
29. Fujiwara, T., K. Ritchie, H. Murakoshi, K. Jacobson, and A. Kusumi. 2002. Phospholipids undergo hop diffusion in compartmentalized cell membrane. J. Cell Biol. 157:1071-1081. (Pubitemid 34839781)
30. Murase, K., T. Fujiwara, Y. Umemura, K. Suzuki, R. Iino, et al. 2004. Ultrafine membrane compartments for molecular diffusion as revealed by single molecule techniques. Biophys. J. 86:4075-4093. (Pubitemid 38780282)
31. Suzuki, K., K. Ritchie, E. Kajikawa, T. Fujiwara, and A. Kusumi. 2005. Rapid hop diffusion of a G-protein-coupled receptor in the plasma membrane as revealed by single-molecule techniques. Biophys. J. 88:3659-3680. (Pubitemid 40586614)
32. Prior, I. A., C. Muncke, R. G. Parton, and J. F. Hancock. 2003. Direct visualization of Ras proteins in spatially distinct cell surface microdomains. J. Cell Biol. 160:165-170.
33. Rotblat, B., I. A. Prior, C. Muncke, R. G. Parton, Y. Kloog, et al. 2004. Three separable domains regulate GTP-dependent association of H-ras with the plasma membrane. Mol. Cell. Biol. 24:6799-6810.
34. Tokunaga, M., N. Imamoto, and K. Sakata-Sogawa. 2008. Highly inclined thin illumination enables clear single-molecule imaging in cells. Nat. Methods. 5:159-161.
35. Haldi, M., C. Ton, W. L. Seng, and P. McGrath. 2006. Human melanoma cells transplanted into zebrafish proliferate, migrate, produce melanin, form masses and stimulate angiogenesis in zebrafish. Angiogenesis. 9:139-151. (Pubitemid 46232205)
36. Mathias, J. R., M. E. Dodd, K. B. Walters, J. Rhodes, J. P. Kanki, et al. 2007. Live imaging of chronic inflammation caused by mutation of zebrafish Hai1. J. Cell Sci. 120:3372-3383.
37. Kawakami, A., T. Fukazawa, and H. Takeda. 2004. Early fin primordia of zebrafish larvae regenerate by a similar growth control mechanism with adult regeneration. Dev. Dyn. 231:693-699.