Ultrastructural analysis of nanogold-labeled endocytic compartments of yeast Saccharomyces cerevisiae using a cryosectioning procedure

Journal of Histochemistry and Cytochemistry

Volumn 57, Issue 8, 2009, Pages 801-809

  Griffith, Janice ^a   Reggiori, Fulvio ^a  

^a UNIVERSITY MEDICAL CENTER UTRECHT   (Netherlands)

Author keywords

Electron microscopy; Endocytosis; Endosomes; Immunogold labeling; Positively charged nanogold; Tokuyasu cryosectioning; Vacuole; Yeast

Indexed keywords

ARTICLE; BIOGENESIS; CELL COMPARTMENTALIZATION; CELL ULTRASTRUCTURE; ENDOCYTOSIS; ENDOSOME; IMMUNOELECTRON MICROSCOPY; IMMUNOGOLD LABELING; MEMBRANE TRANSPORT; NONHUMAN; PRIORITY JOURNAL; PROTEIN LOCALIZATION; SACCHAROMYCES CEREVISIAE; TISSUE SECTION; GENETICS; INTRACELLULAR MEMBRANE; METABOLISM; MUTATION; ULTRAMICROTOMY; ULTRASTRUCTURE;

EUKARYOTA; SACCHAROMYCES CEREVISIAE;

GOLD; METAL NANOPARTICLE;

CRYOULTRAMICROTOMY; ENDOCYTOSIS; ENDOSOMES; GOLD; INTRACELLULAR MEMBRANES; METAL NANOPARTICLES; MICROSCOPY, IMMUNOELECTRON; MUTATION; SACCHAROMYCES CEREVISIAE;

EID: 69249222818     PISSN: 00221554     EISSN: None     Source Type: Journal    
DOI: 10.1369/jhc.2009.952952     Document Type: Article

References (37)

1. Babst M, Katzmann DJ, Estepa-Sabal EJ, Meerloo T, Emr SD (2002) ESCRT-III: an endosome-associated heterooligomeric protein complex required for MVB sorting. Dev Cell 3:271-282
2. Babst M, Sato TK, Banta LM, Emr SD (1997) Endosomal transport function in yeast requires a novel AAA-type ATPase, Vps4p. EMBO J 16:1820-1831
3. Babst M, Wendland B, Estepa EJ, Emr SD (1998) The Vps4p AAA ATPase regulates membrane association of a Vps protein complex required for normal endosome function. EMBO J 17:2982-2993
4. Bowers K, Stevens TH (2005) Protein transport from the late Golgi to the vacuole in the yeast Saccharomyces cerevisiae. Biochim Biophys Acta 1744:438-454
5. Conibear E, Stevens TH (1998) Multiple sorting pathways between the late Golgi and the vacuole in yeast. Biochim Biophys Acta 1404:211-230
6. Darsow T, Rieder SE, Emr SD (1997) A multispecificity syntaxin homologue, Vam3p, essential for autophagic and biosynthetic protein transport to the vacuole. J Cell Biol 138:517-529
7. Gauss R, Trautwein M, Sommer T, Spang A (2005) New modules for the repeated internal and N-terminal epitope tagging of genes in Saccharomyces cerevisiae. Yeast 22:1-12
8. Griffith J, Mari M, De Maziere A, Reggiori F (2008) A cryosectioning procedure for the ultrastructural analysis and the immunogold labelling of yeast Saccharomyces cerevisiae. Traffic 9:1060-1072
9. Griffiths GM (1983) Selective contrast for electron microscopy using thawed frozen sections and immunocytochemistry. In Revel JP, Barnard T, Haggis GH, eds. Science of Biological Specimen Preparation. AMF O'Hare, SEM, 153-159
10. Hicke L (1997) Ubiquitin-dependent internalization and downregulation of plasma membrane proteins. FASEB J 11:1215-1226
11. Hicke L (1999) Gettin' down with ubiquitin: turning off cell-surface receptors, transporters and channels. Trends Cell Biol 9:107-112
12. Huckaba TM, Gay AC, Pantalena LF, Yang HC, Pon LA (2004) Live cell imaging of the assembly, disassembly, and actin cabledependent movement of endosomes and actin patches in the budding yeast, Saccharomyces cerevisiae. J Cell Biol 167:519-530
13. Hurley JH, Emr SD (2006) The ESCRT complexes: structure and mechanism of a membrane-trafficking network. Annu Rev Biophys Biomol Struct 35:277-298
14. Jones EW (1977) Proteinase mutants of Saccharomyces cerevisiae. Genetics 85:23-33
15. Katzmann DJ, Odorizzi G, Emr SD (2002) Receptor downregulation and multivesicular-body sorting. Nat Rev Mol Cell Biol 3:893-905
16. Lewis MJ, Nichols BJ, Prescianotto-Baschong C, Riezman H, Pelham HR (2000) Specific retrieval of the exocytic SNARE Snc1p from early yeast endosomes. Mol Biol Cell 11:23-38
17. Longtine MS, McKenzie A III, Demarini DJ, Shah NG, Wach A, Brachat A, Philippsen P, et al. (1998) Additional modules for versatile and economical PCR-based gene deletion and modification in Saccharomyces cerevisiae. Yeast 14:953-961
18. Luzio JP, Pryor PR, Bright NA (2007) Lysosomes: fusion and function. Nat Rev Mol Cell Biol 8:622-632
19. Munn AL (2000) The yeast endocytic membrane transport system. Microsc Res Tech 51:547-562
20. Odorizzi G, Babst M, Emr SD (1998) Fab1p PtdIns(3)P 5-kinase function essential for protein sorting in the multivesicular body. Cell 95:847-858
21. Osumi M, Konomi M, Sugawara T, Takagi T, Baba M (2006) High-pressure freezing is a powerful tool for visualization of Schizosaccharomyces pombe cells: ultra-low temperature and lowvoltage scanning electron microscopy and immunoelectron microscopy. J Electron Microsc (Tokyo) 55:75-88
22. Peplowska K, Markgraf DF, Ostrowicz CW, Bange G, Ungermann C (2007) The CORVET tethering complex interacts with the yeast Rab5 homolog Vps21 and is involved in endo-lysosomal biogenesis. Dev Cell 12:739-750
23. Prescianotto-Baschong C, Riezman H (1998) Morphology of the yeast endocytic pathway. Mol Biol Cell 9:173-189
24. Prescianotto-Baschong C, Riezman H (2002) Ordering of compartments in the yeast endocytic pathway. Traffic 3:37-49
25. Robinson JS, Klionsky DJ, Banta LM, Emr SD (1988) Protein sorting in Saccharomyces cerevisiae: isolation of mutants defective in the delivery and processing of multiple vacuolar hydrolases. Mol Cell Biol 8:4936-4948
26. Rothman JH, Howald I, Stevens TH (1989) Characterization of genes required for protein sorting and vacuolar function in the yeast Saccharomyces cerevisiae. EMBO J 8:2057-2065
27. Seron K, Tieaho V, Prescianotto-Baschong C, Aust T, Blondel MO, Guillaud P, Devilliers G, et al. (1998) Ayeast t-SNARE involved in endocytosis. Mol Biol Cell 9:2873-2889
28. Shaw JD, Cummings KB, Huyer G, Michaelis S, Wendland B (2001) Yeast as a model system for studying endocytosis. Exp Cell Res 271:1-9
29. Sipos G, Brickner JH, Brace EJ, Chen L, Rambourg A, Kepes F, Fuller RS (2004) Soi3p/Rav1p functions at the early endosome to regulate endocytic trafficking to the vacuole and localization of trans-Golgi network transmembrane proteins. Mol Biol Cell 15:3196-3209
30. Slot JW, Geuze HJ (2007) Cryosectioning and immunolabeling. Nat Protocols 2:2480-2491
31. Slot JW, Geuze HJ, Weerkaamp AJ (1988) Localization of macromolecular components by application of the immunogold technique on cryosectioned bacteria. Methods Microbiol 20:211-236
32. Tokuyasu KT (1978) A study of positive staining of ultrathin frozen sections. J Ultrastruct Res 63:287-307
33. van Donselaar E, Posthuma G, Zeuschner D, Humbel BM, Slot JW (2007) Immunogold labeling of cryosections from high-pressure frozen cells. Traffic 8:471-485
34. van Meel E, Klumperman J (2008) Imaging and imagination: understanding the endo-lysosomal system. Histochem Cell Biol 129:253-266
35. Vida TA, Emr SD (1995) A new vital stain for visualizing vacuolar membrane dynamics and endocytosis in yeast. J Cell Biol 128:779-792
36. Wada Y, Nakamura N, Ohsumi Y, Hirata A (1997) Vam3p, a new member of syntaxin related protein, is required for vacuolar assembly in the yeast Saccharomyces cerevisiae. J Cell Sci 110:1299-1306
37. Wang C-W, Stromhaug PE, Shima J, Klionsky DJ (2002) The Ccz1-Mon1 protein complex is required for the late step of multiple vacuole delivery pathways. J Biol Chem 277:47917-47927