Single-layer centrifugation with Androcoll-E can be scaled up to allow large volumes of stallion ejaculate to be processed easily

Theriogenology

Volumn 72, Issue 6, 2009, Pages 879-884

  Morrell, J M ^a   Johannisson, A ^a   Dalin, A M ^a   Rodriguez Martinez, H ^a  

^a SWEDISH UNIVERSITY OF AGRICULTURAL SCIENCES   (Sweden)

Author keywords

Chromatin integrity; Morphology; Scale up; SLC; Sperm motility; Stallion

Indexed keywords

SILICON DIOXIDE;

ANIMAL; ARTICLE; CENTRIFUGATION; CHEMISTRY; CHROMATIN; COLLOID; HORSE; MALE; PHYSIOLOGY; SPERMATOZOON; SPERMATOZOON MOTILITY; TRANSPLANTATION;

ANIMALS; CENTRIFUGATION; CHROMATIN; COLLOIDS; HORSES; MALE; SILICON DIOXIDE; SPERM MOTILITY; SPERMATOZOA; TISSUE AND ORGAN HARVESTING;

EQUUS CABALLUS;

EID: 69249216432     PISSN: 0093691X     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.theriogenology.2009.05.015     Document Type: Article

References (15)

1. Morrell J.M., Dalin A.-M., and Rodriguez-Martinez H. Prolongation of stallion sperm survival by centrifugation through coated silica colloids: a preliminary study. Anim Reprod 5 (2008) 121-126
2. Morrell J.M., Johannisson A., Dalin A.-M., and Rodriguez-Martinez H. Morphology and chromatin integrity of stallion spermatozoa prepared by density gradient and single layer centrifugation through silica colloids. Reprod Domest Anim 44 (2008) 512-517
3. Morrell J.M., Dalin A.-M., and Rodriguez-Martinez H. Comparison of density gradient and single layer centrifugation of stallion spermatozoa: yield, motility and survival. Equine Vet J 41 (2009) 53-58
4. Macpherson M., Blanchard T.L., Love C.C., Brinsko S.P., Thompson J.A., and Varner D.D. Use of a silane-coated silica particle solution to enhance the quality of ejaculated semen in stallions. Theriogenology 58 (2002) 317-320
5. Morrell J.M. Update on semen technologies for animal breeding. Reprod Domest Anim 40 (2006) 1-5
6. Kenney R.M., Bergman R.V., Cooper W.L., and Morse G.W. Minimal contamination techniques for breeding mares: techniques and preliminary findings. Proc Am Assoc Equine Practice 2 (1975) 327-336
7. Morrell J.M., Johannisson A., Strutz H., Dalin A.-M., and Rodriguez-Martinez H. Colloidal centrifugation of stallion semen: changes in sperm motility, velocity and chromatin integrity during storage. J Equine Vet Sci 29 (2009) 24-32
8. Kavak A., Johannisson A., Lundeheim N., Rodriguez-Martinez H., Aidnik M., and Einarsson S. Evaluation of cryopreserved stallion semen from Tori and Estonian breeds using CASA and flow cytometry. Anim Reprod Sci 76 (2003) 205-216
9. Johannisson A., Morrell J.M., Thorén J., Jonsson M., Dalin A.-M., and Rodriguez-Martinez H. Colloidal centrifugation with Androcoll™-E prolongs stallion sperm motility, viability and chromatin integrity. Anim Reprod Sci (2009) j.anireprosci.2009.01.008
10. Lagerlöf N. Morphological studies on the change in sperm structure and in the testes of bulls with decreased or abolished fertility. Acta Pathol Microbiol Scand Suppl 19 (1934) 254-267
11. Evenson D.P., Darzynkiewicz Z., and Melamed M.R. Relation of mammalian sperm chromatin heterogeneity to fertility. Science 210 (1980) 1131-1133
12. Januskauskas A., Gil J., Söderquist L., Håård M.G.M., Håård MCh, Johannisson A., and Rodriguez-Martinez H. Effect of cooling rates on post-thaw sperm motility, membrane integrity, capacitation status and fertility of dairy bull semen used for artificial insemination in Sweden. Theriogenology 52 (1999) 641-658
13. Rodriguez-Martinez H., Larsson B., and Pertoft H. Evaluation of sperm damage and techniques for sperm clean-up. Reprod Fertil Dev 9 (1997) 297-308
14. Edmond A.J., Teague A.R., Brinsko S.P., Comerford K.L., Waite J.A., Mancill S.S., et al. Effect of density gradient centrifugation on quality and recovery of equine spermatozoa. Anim Reprod Sci 107 (2008) 318
15. Christanelli M.J., Squire E.L., Amann R.P., and Pickett B.W. Fertility of stallion semen processed, frozen and thawed by a new procedure. Theriogenology 22 (1984) 39-45