Interaction trap/two-hybrid system to identify interacting proteins

Current Protocols in Protein Science

Volumn , Issue SUPPL. 57, 2009, Pages

  Golemis, Erica A ^a   Serebriiskii, Ilya ^a   Finley, Russell L ^b   Kolonin, Mikhail G ^b   Gyuris, Jeno ^c   Brent, Roger ^d  

^a FOX CHASE CANCER CENTER   (United States)

^b WAYNE STATE UNIVERSITY SCHOOL OF MEDICINE   (United States)

^c GPC Biotech Inc   (United States)

^d The Molecular Sciences Institute   (United States)

Author keywords

Interaction mating; Interaction trap; Protein interactions; Yeast two hybrid

Indexed keywords

BETA GALACTOSIDASE; COMPLEMENTARY DNA; FUNGAL PROTEIN; HYBRID PROTEIN; LEXA PROTEIN; PLASMID DNA; REAGENT;

DNA LIBRARY; DNA PURIFICATION; ESCHERICHIA COLI; FUNGAL STRAIN; FUNGUS CULTURE; FUNGUS GROWTH; GENE CONSTRUCT; GENE REPRESSION; IMMUNOBLOTTING; NONHUMAN; PLASMID; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROTEIN DETERMINATION; PROTEIN DNA BINDING; PROTEIN INTERACTION; PROTEIN SYNTHESIS; REPORTER GENE; REVIEW; SCREENING TEST; TRANSCRIPTION INITIATION; TWO HYBRID SYSTEM; ANIMAL CELL; ARTICLE; DNA BINDING; LABORATORY DEVICE; MALE; MOLECULAR CLONING; PROTEIN ANALYSIS; PROTEIN DATABASE; SALMONINE; SPERM; YEAST;

ANIMALS; CLONING, MOLECULAR; GENE EXPRESSION; GENE LIBRARY; HUMANS; PLASMIDS; PROTEIN BINDING; PROTEINS; RECOMBINANT FUSION PROTEINS; TWO-HYBRID SYSTEM TECHNIQUES; YEASTS;

EID: 69249134282     PISSN: 19343655     EISSN: 19343663     Source Type: Journal    
DOI: 10.1002/0471140864.ps1902s57     Document Type: Review

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