Production of extracellular anti-leukaemic enzyme L-asparaginase from marine actinomycetes by solid-state and submerged fermentation: Purification and characterisation

Tropical Journal of Pharmaceutical Research

Volumn 8, Issue 4, 2009, Pages 353-360

  Saleem Basha, N ^a   Rekha, R ^a   Komala, M ^a   Ruby, S ^a  

^a Mohamed Sathak A J College of Pharmacy   (India)

Author keywords

Km and Vmax; L asparaginase; SDS PAGE; Solid state media; TFY broth; TGY broth

Indexed keywords

AMMONIUM SULFATE; ANTILEUKEMIC AGENT; ASPARAGINASE; SEPHADEX;

ACTINOBACTERIA; ARTICLE; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME ISOLATION; ENZYME SPECIFICITY; ENZYME SYNTHESIS; GEL FILTRATION; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; SOLID STATE FERMENTATION; SUBMERGED FERMENTATION;

EID: 69249105592     PISSN: 15965996     EISSN: 15969827     Source Type: Journal    
DOI: None     Document Type: Article

References (27)

1. Lehninger AL. Principles of biochemistry. New Delhi: CBS Publishers; 1990. p. 207-247.
2. Trevan M, Boffey S, Goulding KH, Stanbury P. Biotechnology; the Biological principles. New Delhi: Tata Mc Graw-Hill Publishing Ltd; 2004. p. 155-228.
3. Amy LS, Mariusz J, Dominique H, Mohana Rao JK. Crystal structure of Escherichia coli LAsparaginase, an enzyme used in cancer therapy. Proc Natl Acad Sci, USA. 1993; 90:1474-1478.
4. Surajit D, Lyla PS, Ajmal Khan S. Marine microbial diversity and ecology: Importance and future perspectives. Current Science 2006; 90:1325-1335.
5. Albert L, Simon S. Pharmaceutical Enzymes. New York: Marcel Dekker Inc Publishing; 1997. p. 223-260.
6. Dhevagi P, Poorani E. Isolation and characterization of L-Asparaginase from marine actinomycetes. Ind J Biotech 2005; 5:514-520. (Pubitemid 44862462)
7. Tetsuya T, Ryujiro S, Kozo Y, Masatoshi N, Katsuka A, Ichiro C. L-Asparaginase from Proteus vulgaris. J App Microbiol 1971; 3:387-392.
8. Yasser R, Abdel-Fattah, Zakia A, . L-Asparaginase production by Pseudomonas aeruginosa in solid-state culture: Evaluation and optimization of culture conditions using factorial designs. Prospects in Biochem. 2002; 38:115-122.
9. th Edition. UK: Pergamon press; 1984. p.157-161.
10. Shirling ED, Gottlieb D. Methods for characterization of Streptomyces sp. Int J Syst Bacterial 1996; 6:313-340.
11. Ted RJ and Case CL. Laboratory Experiments in Microbiology. 4th Edition. United States: The Benjamin/Cummings Publishing Company Inc; 1995. p. 27-44.
12. th International Student Edition; 2003. p. 135-186.
13. Gulati R, Saxena RK, Gupta R. A rapid plate assay for screening L-asparaginase producing microorganisms. Lett Appl Microbiol 1997; 24:23-26. (Pubitemid 27064563)
14. Peterson RE, Ciegler. L-asparaginase production by Erwinia aroideae. J Appl Microbiol 1969; 18:64-67.
15. Lois D, Russell D, Peggy W, James H, Barrie G. Purification and properties of an Lglutaminase- L-asparaginase from Pseudomonas acidovorans. J Bacteriol 1977; 129:1379-1386. (Pubitemid 8109678)
16. Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. Protein measurement with the folin-phenol reagent. J. Biol. Chem. 1951; 48:17-25.
17. Asparaginase Assay. Worthington enzyme manual 1993, Published by Worthington Biochemical Corporation. Available from: http://www. worthingtonbiochem. com/index/manual.html . Accessed 20 August 2007
18. David K, David G. L-Asparaginase Production by the rumen anaerobe Vibrio succinogenes. J Appl Microbiol 1974; 27:206-209.
19. Peter JD. L-asparaginase production by Streptomyces griseus. J Appl Microbiol 1972; 23:1163-1164.
20. John WB, Arthur WP. Purification and properties of L-asparaginase from Serratia marcescens. J Bacteriol 1971; 106:578-587.
21. Gaffer SA, Shethna YI. Partial purification and antitumour activity of L-asparaginase from Azotobacter vinelandi. J Appl Microbiol 1975; 44:727-729.
22. Sambrook J, David W Russel. Molecular cloning- A laboratory manual. 3rd Edition. New York: Cold Spring Harbor Laboratory Press; 2003. p. A8.40 - A8.51.
23. Bernard H and Alma JH. Production of tumorinhibitory L-asparaginase by submerged growth of Serratia marcescens. J Appl Microbiol 1969; 18(4):550-554.
24. Wriston JC and Yellin TO. L-asparaginase -A review. Adv Enzymol Relat Areas. Mol. Biol 1973; 39:185-249.
25. James HS, Jane YR and Broome JD. Two Lasparaginases from E. coli and their action against tumors. J Appl Microbiol 1966; 56:1516-1519.
26. Scheetz RW, Whelan HA and Wriston JC. Purification and properties of an Lasparaginase from Fusarium tricinctum 1971; 142: 184-189.
27. Tosa T, Sano R, Yamamoto K, Nakamura M and Chibata I. L-asparaginase from Proteus vulgaris; purification, crystallization and enzymic properties. J Biochem 1972; 11:217-222.