Purification, immobilization, and characterization of nattokinase on PHB nanoparticles

Bioresource Technology

Volumn 100, Issue 24, 2009, Pages 6644-6646

  Deepak, Venkataraman ^a   Ram Kumar Pandian, Suresh babu ^a   Kalishwaralal, Kalimuthu ^a   Gurunathan, Sangiliyandi ^a  

^a KALASALINGAM UNIVERSITY   (India)

Author keywords

Immobilization; Nattokinase; PHB nanoparticles; Polyhydroxybutyrate

Indexed keywords

BACILLUS SUBTILIS; BREVIBACTERIUM CASEI; DAIRY WASTES; ENHANCED STABILITY; FTIR ANALYSIS; IMMOBILIZATION; ION EXCHANGE CHROMATOGRAPHY; NANOPRECIPITATION; NATTOKINASE; NATURAL BIOPOLYMERS; NOVEL STRAINS; PHB NANOPARTICLES; POLYHYDROXYALKANOATES; POLYHYDROXYBUTYRATE;

AGRICULTURAL WASTES; BACTERIOLOGY; CHROMATOGRAPHIC ANALYSIS; ENZYME ACTIVITY; ENZYMES; ION CHROMATOGRAPHY; ION EXCHANGE; NANOPARTICLES; PURIFICATION; TRANSMISSION ELECTRON MICROSCOPY;

ENZYME IMMOBILIZATION;

FIBRINOLYTIC AGENT; NANOPARTICLE; NATTOKINASE; POLY(3 HYDROXYBUTYRIC ACID); POLYHYDROXYALKANOIC ACID; UNCLASSIFIED DRUG;

FTIR SPECTROSCOPY; IMMOBILIZATION; PARTICLE SIZE; TRANSMISSION ELECTRON MICROSCOPY;

ARTICLE; BACILLUS SUBTILIS; BACTERIUM CULTURE; BACTERIUM ISOLATE; BREVIBACTERIUM; BREVIBACTERIUM CASEI; CONTROLLED STUDY; DRUG FORMULATION; ENZYME ACTIVITY; ENZYME IMMOBILIZATION; ENZYME PURIFICATION; ENZYME STABILITY; NONHUMAN; PARTICLE SIZE; PRIORITY JOURNAL; STORAGE TEMPERATURE;

BACILLUS SUBTILIS; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ENZYME STABILITY; ENZYMES, IMMOBILIZED; HYDROGEN-ION CONCENTRATION; NANOPARTICLES; POLYESTERS; POLYHYDROXYALKANOATES; SUBTILISINS; TEMPERATURE;

BACILLUS SUBTILIS; BREVIBACTERIUM CASEI;

EID: 69049101903     PISSN: 09608524     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.biortech.2009.06.057     Document Type: Article

References (17)

1. Berlanga M., Montero M.T., Borrel J.H., and Guerrero R. Rapid spectrofluorometric screening of poly-hydroxyalkanoate-producing bacteria from microbial mats. International Microbiology 92 (2006) 95-102
2. Deepak V., Kalishwaralal K., Ramkumarpandian S., Babu S.V., Senthilkumar S.R., and Sangiliyandi G. Optimization of media composition for nattokinase production by Bacillus subtilis using response surface methodology. Bioresource Technology 99 (2008) 8170-8174
3. Fessi H., Puisieux F., Devissaguet J.P., Ammoury N., and Benita S. Nanocapsule formation by interfacial polymer deposition following solvent displacement. International Journal of Pharmaceutics 55 (1989) R1-R4
4. Fujita M., Nomura K., Hong K., Ito Y., Asada A., and Mishimuro S. Purification and characterization of the strong fibrinolytic enzyme nattokinase in the vegetable cheese natto, a popular soybean fermented food in Japan. Biochemical and Biophysical Research Communications 197 (1993) 1340-1347
5. Gianfreda L., and Scarfi M.R. Enzyme stabilization: state of the art. Molecular and Cellular Biochemistry 100 (1991) 97-128
6. Hans M.L., and Lowmanm A.M. Biodegradable nanoparticles for drug delivery and targeting. Current Opinion in Solid State Mechanics 63 (2002) 19-327
7. Hong K., Sun S., Tian W., Chen G.Q., and Huang W. A rapid method for detecting bacterial polyhydroxyalkanoates in intact cells by Fourier transform infrared spectroscopy. Applied Microbiology and Biotechnology 51 (1999) 523-526
8. Hsieh C.W., Lu W.C., Hsieh W.C., Huang Y.P., Lai C.H., and Ko W.C. Improvement of the stability of nattokinase using γ-polyglutamic acid as a coating material for microencapsulation. LWT - Food Science and Technology 42 (2009) 144-149
9. Mateo C., Palomo J.M., Fernandez-Lorente G., Guisan J.M., and Fernandez-Lafuente R. Improvement of enzyme activity, stability and selectivity via immobilization techniques. Enzyme and Microbial Technology 40 (2007) 1451-1463
10. Omura K., Hitosugi M., Zhu X., Ikeda M., and Maeda H. A newly derived protein from Bacillus subtilis natto with both antithrombotic and fibrinolytic effects. Journal of Pharmacological Sciences 99 (2005) 247-251
11. Peng Y., Yang X., and Zhang Y. Microbial fibrinolytic enzymes: an overview of source, production, properties, and thrombolytic activity in vivo. Applied Microbiology and Biotechnology 69 (2005) 126-132
12. Sumi H., Hamada H., Tsushima H., Mihara H., and Muraki H. A novel fibrinolytic enzyme nattokinase in the vegetable cheese natto; a typical and popular soybean food in the Japanese diet. Experientia 15 (1987) 1110-1111
13. Sumi H., Hamada H., Nakanishi K., and Hiratani H. Enhancement of the fibrinolytic activity in plasma by oral administration of nattokinase. Acta Haematologica 843 (1990) 139-143
14. Tamber H., Johansen P., Merkle H.P., and Gander B. Formulation aspects of biodegradable polymeric microspheres for antigen delivery. Advanced Drug Delivery Reviews 57 (2005) 357-376
15. Verlinden R.A.J., Hill D.J., Kenward M.A., Williams C.D., and Radecka I. Bacterial synthesis of biodegradable polyhydroxyalkanoates. Journal of Applied Microbiology 102 (2007) 1437-1449
16. Volova T.G., Shishatskaya E.I., Sevastianov V.I., Efremov S., and Mogilnaya O. Results of biomedical investigations of PHB and PHB/PHV fibers. Biochemical Engineering Journal 16 (2003) 125-133
17. Zanetti-Ramos B.G., Lemos-Senna E., Cramail H., Cloutet E., Borsali R., and Soldi V. The role of surfactant in the miniemulsion polymerization of biodegradable polyurethane nanoparticles. Materials Science and Engineering, C: Biomimetic and Supramolecular Systems 28 4 (2008) 526-531