Assessing amino acid uptake by phototrophic nanoflagellates in nonaxenic cultures using flow cytometric sorting

FEMS Microbiology Letters

Volumn 298, Issue 2, 2009, Pages 166-173

  Hartmann, Manuela ^a   Zubkov, Mikhail V ^a   Martin, Adrian P ^a   Scanlan, David J ^b   Burkill, Peter H ^c,d  

^a UNIVERSITY OF SOUTHAMPTON   (United Kingdom)

^b UNIVERSITY OF WARWICK   (United Kingdom)

^c THE LABORATORY   (United Kingdom)

^d UNIVERSITY OF PLYMOUTH   (United Kingdom)

Author keywords

Algal osmotrophy; Isotopic tracer; Organic nitrogen; Plastidic protists

Indexed keywords

AMINO ACID; LEUCINE; ORGANIC NITROGEN;

ALGAL CELL CULTURE; AMINO ACID TRANSPORT; ANIMAL CELL; ARTICLE; BACTERIUM CULTURE; CELL SELECTION; CONCENTRATION PROCESS; CONTROLLED STUDY; EUKARYOTIC CELL; FLAGELLATE; FLOW CYTOMETRY; GERMFREE ANIMAL; MICROBIAL POPULATION DYNAMICS; NANOFLAGELLATE; NONHUMAN; PHOTOTROPHIC BACTERIUM; PRIORITY JOURNAL; PROKARYOTE; PROKARYOTIC CELL; PROTISTAN COMMUNITY;

ALGAE, GREEN; BACTERIA; CARBON RADIOISOTOPES; FLOW CYTOMETRY; LEUCINE; STAINING AND LABELING; TRITIUM;

ALGAE; BACTERIA (MICROORGANISMS); EUKARYOTA; PELAGOPHYCEAE; PRASINOPHYCEAE; PROKARYOTA; PROTISTA;

EID: 68949217201     PISSN: 03781097     EISSN: 15746968     Source Type: Journal    
DOI: 10.1111/j.1574-6968.2009.01715.x     Document Type: Article

References (35)

1. Alderkamp A-C, Sintes E Herndl GJ (2006) Abundance and activity of major groups of prokaryotic plankton in the coastal North Sea during spring and summer. Aquat Microb Ecol 45 : 237 246.
2. Azam F Hodson RE (1977) Size distribution and activity of marine microheterotrophs. Limnol Oceanogr 22 : 492 501.
3. Azam F Hodson RE (1981) Multiphasic kinetics for d-glucose uptake by assemblages of natural marine bacteria. Mar Ecol-Prog Ser 6 : 213 222.
4. Berman T (1975) Size fractionation of natural aquatic populations associated with autotrophic and heterotrophic carbon uptake. Mar Biol 33 : 215 220.
5. Droop MR (1974) Heterotrophy of carbon. Algal Physiology and Biochemistry (Stewart WP, ed.), pp. 530 559. Blackwell, Oxford.
6. Fenchel T (1987) Ecology of Protozoa. The Biology of Free-Living Phagotrophic Protists. Science Tech Publishers, Madison, WI.
7. Flynn KJ Butler I (1986) Nitrogen-sources for the growth of marine microalgae - role of dissolved free amino-acids. Mar Ecol-Prog Ser 34 : 281 304.
8. Glibert P Legrand C (2006) The diverse nutrient strategies of harmful algae: focus on osmotrophy. Ecology of Harmful Algae (Granéli E Turner J, eds.), pp. 163 176. Springer, New York, NY.
9. Grossart HP, Kiorboe T, Tang KW, Allgaier M, Yam EM Ploug H (2006) Interactions between marine snow and heterotrophic bacteria: aggregate formation and microbial dynamics. Aquat Microb Ecol 42 : 19 26.
10. Iturriaga R Zsolnay A (1981) Differentiation between auto- and heterotrophic activity: problems in the use of size fractionation and antibiotics. Bot Mar 24 : 399 404.
11. John EH Flynn KJ (1999) Amino acid uptake by the toxic dinoflagellate Alexandrium fundyense. Mar Biol 133 : 11 19.
12. Kamjunke N Tittel J (2008) Utilisation of leucine by several phytoplankton species. Limnologica 38 : 360 366.
13. Keller MD, Selvin RC, Claus W Guillard RRL (1987) Media for the culture of oceanic ultraphytoplankton. J Phycol 23 : 633 638.
14. Lee JJ, McEnery ME, Kennedy EM Rubin H (1973) Deducing the functional relationships among the diatom assemblages within sublittoral salt marsh epiphytic communities. Bull Ecol Res Comm 17 : 387 397.
15. Lewitus AJ (2006) Osmotrophy in marine microalgae. Algal Cultures, Analogues of Blooms and Applications (Subba-Rao DV, ed.), pp. 343 383. Science Publishers, Enfield, NH.
16. Li WKW Dickie PM (1985a) Metabolic Inhibition of size-fractionated marine plankton radiolabeled with amino-acids, glucose, bicarbonate, and phosphate in the light and dark. Microb Ecol 11 : 11 24.
17. Li WKW Dickie PM (1985b) Growth of bacteria in seawater filtered through 0.2 μm nuclepore membranes: implications for dilution experiments. Mar Ecol-Prog Ser 26 : 245 252.
18. Linares F Sundbäck K (2006) Uptake of dissolved free amino acids (DFAA) by microphytobenthic communities. Aquat Microb Ecol 42 : 175 186.
19. Marie D, Partensky F, Jacquet S Vaulot D (1997) Enumeration and cell cycle analysis of natural populations of marine picoplankton by flow cytometry using the nucleic acid stain SYBR Green I. Appl Environ Microb 63 : 186 193.
20. Mary I, Tarran GA, Warwick PE, Terry MJ, Scanlan DJ, Burkill PH Zubkov MV (2008) Light enhanced amino acid uptake by dominant bacterioplankton groups in surface waters of the Atlantic Ocean. FEMS Microbiol Ecol 63 : 36 45.
21. Nielsen JL, Christensen D, Kloppenborg M Nielsen PH (2003) Quantification of cell-specific substrate uptake by probe-defined bacteria under in situ conditions by microautoradiography and fluorescence in situ hybridization. Environ Microbiol 5 : 202 211.
22. Nilsson C Sundbäck K (1996) Amino acid uptake in natural microphytobenthic assemblages studied by microautoradiography. Hydrobiologia 332 : 119 129.
23. Palenik B Morel FMM (1990) Amino acid utilization by marine phytoplankton: a novel mechanism. Limnol Oceanogr 35 : 260 269.
24. Palmisano AC, Kottmeier ST, Moe RL Sullivan CW (1985) Sea ice microbial communities.4. The effect of light perturbation on microalgae at the ice-seawater interface in McMurdo Sound, Antarctica. Mar Ecol-Prog Ser 21 : 37 45.
25. Pollingher U Berman T (1976) Autoradiographic screening for potential heterotrophs in natural algal populations of Lake Kinneret. Microb Ecol 2 : 252 260.
26. Rivkin RB (1986) Incorporation of tritiated thymidine by eukaryotic microalgae. J Phycol 22 : 193 198.
27. Rivkin RB Putt M (1987) Heterotrophy and photoheterotrophy by antarctic microalgae - light-dependent incorporation of amino-acids and glucose. J Phycol 23 : 442 452.
28. Saunders GW (1972) Potential heterotrophy in a natural population of Oscillatoria agardhii var isothrix skuja. Limnol Oceanogr 17 : 704 711.
29. Shapiro HM (2003) Practical Flow Cytometry. John Wiley & Sons Inc, New York.
30. Tittel J, Bissinger V, Zippel B, Gaedke U, Bell E, Lorke A Kamjunke N (2003) Mixotrophs combine resource use to outcompete specialists: implications for aquatic food webs. P Natl Acad Sci USA 100 : 12776 12781.
31. Watson SW, Novitsky TJ, Quinby HL Valois FW (1977) Determination of bacterial number and biomass in the marine environment. Appl Environ Microb 33 : 940 946.
32. Wright RT Hobbie JE (1966) Use of glucose and acetate by bacteria and algae in aquatic ecosystems. Ecology 47 : 447 464.
33. Zotina T, Koster O Juttner F (2003) Photoheterotrophy and light-dependent uptake of organic and organic nitrogenous compounds by Planktothrix rubescens under low irradiance. Freshwater Biol 48 : 1859 1872.
34. Zubkov MV Sleigh MA (2005) Assimilation efficiency of Vibrio bacterial protein biomass by the flagellate Pteridomonas: assessment using flow cytometric sorting. FEMS Microbiol Ecol 54 : 281 286.
35. Zubkov MV, Tarran GA, Mary I Fuchs BM (2008) Differential microbial uptake of dissolved amino acids and amino sugars in surface waters of the Atlantic Ocean. J Plankton Res 30 : 211 220.