Precise gene-dose alleles for chemical genetics

Genetics

Volumn 182, Issue 2, 2009, Pages 623-626

  Yan, Zhun ^a,b   Berbenetz, Nicolas M ^b   Giaever, Guri ^a,b   Nislow, Corey ^a,b  

^a UNIVERSITY OF TORONTO   (Canada)

^b UNIVERSITY OF TORONTO   (Canada)

Author keywords

[No Author keywords available]

Indexed keywords

ALLELE; ARTICLE; CHEMICAL GENETICS; CONTROLLED STUDY; GENE DOSAGE; GENE FUNCTION; HETEROZYGOTE; NONHUMAN; PHARMACOGENETICS; PHENOTYPE; PRIORITY JOURNAL; SACCHAROMYCES CEREVISIAE; DRUG EFFECT; GENE EXPRESSION REGULATION; GENETICS; SENSITIVITY AND SPECIFICITY;

SACCHAROMYCES CEREVISIAE;

FUNGAL RNA; METHOTREXATE;

ALLELES; GENE DOSAGE; GENE EXPRESSION REGULATION, FUNGAL; METHOTREXATE; PHENOTYPE; RNA, FUNGAL; SACCHAROMYCES CEREVISIAE; SENSITIVITY AND SPECIFICITY;

EID: 68949211063     PISSN: 00166731     EISSN: 00166731     Source Type: Journal    
DOI: 10.1534/genetics.109.103036     Document Type: Article

References (18)

1. BAETZ, K., L. MCHARDY, K. GABLE, T. TARLING, D. REBERIOUX et al., 2004 Yeast genome-wide drug-induced haploinsufficiency screen to determine drug mode of action. Proc. Natl. Acad. Sci. USA 101: 4525-4530.
2. BEN-AROYA, S., C. COOMBES, T. KWOK, K. A. O'DONNELL, J. D. BOEKE et al., 2008 Toward a comprehensive temperature-sensitive mutant repository of the essential genes of Saccharomyces cerevisiae. Mol. Cell 30: 248-258.
3. DEUTSCHBAUER, A. M., D. F. JARAMILLO, M. PROCTOR, J. KUMM, M. E. HILLENMEYER et al., 2005 Mechanisms of haploinsufficiency revealed by genome-wide profiling in yeast. Genetics 169: 1915-1925. (Pubitemid 40695608)
4. DOOSTZADEH, J., R. W. DAVIS, G. N. GIAEVER, C. NISLOW and J. W. LANGSTON, 2007 Chemical genomic profiling for identifying intracellular targets of toxicants producing Parkinson's disease. Toxicol. Sci. 95: 182-187.
5. GIAEVER, G., D. D. SHOEMAKER, T. W. JONES, H. LIANG, E. A. WINZELER et al., 1999 Genomic profiling of drug sensitivities via induced haploinsufficiency. Nat. Genet. 21: 278-283.
6. GIAEVER, G., A. M. CHU, L. NI, C. CONNELLY, L. RILES et al., 2002 Functional profiling of the Saccharomyces cerevisiae genome. Nature 418: 387-391. (Pubitemid 34826834)
7. GIAEVER, G., P. FLAHERTY, J. KUMM, M. PROCTOR, C. NISLOW et al., 2004 Chemogenomic profiling: identifying the functional interactions of small molecules in yeast. Proc. Natl. Acad. Sci. USA 101: 793-798.
8. HILLENMEYER, M. E., E. FUNG, J. WILDENHAIN, S. E. PIERCE, S. HOON et al., 2008 The chemical genomic portrait of yeast: uncovering a phenotype for all genes. Science 320: 362-365.
9. LEE, W., R. P. ST. ONGE, M. PROCTOR, P. FLAHERTY, M. I. JORDAN et al., 2005 Genome-wide requirements for resistance to functionally distinct DNA-damaging agents. PLoS Genet. 1: e24.
10. LIVAK, K. J., and T. D. SCHMITTGEN, 2001 Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)). Methods 25: 402-408. (Pubitemid 34164012)
11. LUM, P. Y., C. D. ARMOUR, S. B. STEPANIANTS, G. CAVET, M. K. WOLF et al., 2004 Discovering modes of action for therapeutic compounds using a genome-wide screen of yeast heterozygotes. Cell 116: 121-137.
12. MNAIMNEH, S., A. P. DAVIERWALA, J. HAYNES, J. MOFFAT, W. T. PENG et al., 2004 Exploration of essential gene functions via titratable promoter alleles. Cell 118: 31-44.
13. RAMAKERS, C., J. M. RUIJTER, R. H. DEPREZ and A. F. MOORMAN, 2003 Assumption-free analysis of quantitative real-time polymerase chain reaction (PCR) data. Neurosci. Lett. 339: 62-66.
14. SCHULDINER, M., S. R. COLLINS, N. J. THOMPSON, V. DENIC, A. BHAMIDIPATI et al., 2005 Exploration of the function and organization of the yeast early secretory pathway through an epistatic miniarray profile. Cell 123: 507-519.
15. SOPKO, R., D. HUANG, N. PRESTON, G. CHUA, B. PAPP et al., 2006 Mapping pathways and phenotypes by systematic gene overexpression. Mol. Cell 21: 319-330.
16. STURGEON, C. M., D. KEMMER, H. J. ANDERSON and M. ROBERGE, 2006 Yeast as a tool to uncover the cellular targets of drugs. Biotechnol. J. 1: 289-298.
17. WINZELER, E. A., D. D. SHOEMAKER, A. ASTROMOFF, H. LIANG, K. ANDERSON et al., 1999 Functional characterization of the S. cerevisiae genome by gene deletion and parallel analysis. Science 285: 901-906. (Pubitemid 29381993)
18. YAN, Z., M. COSTANZO, L. E. HEISLER, J. PAW, F. KAPER et al., 2008 Yeast barcoders: a chemogenomic application of a universal donor-strain collection carrying bar-code identifiers. Nat. Methods 5: 719-725.