Prion protein detection via direct immuno-quantitative real-time PCR

Journal of Microbiological Methods

Volumn 78, Issue 3, 2009, Pages 307-311

  Reuter, Tim ^a   Gilroyed, Brandon H ^a   Alexander, Trevor W ^a   Mitchell, Gordon ^b   Balachandran, Aru ^b   Czub, Stefanie ^b   McAllister, Tim A ^a  

^a LETHBRIDGE RESEARCH CENTRE   (Canada)

^b CANADIAN FOOD INSPECTION AGENCY   (Canada)

Author keywords

Immuno quantitative PCR; Prion detection; PVDF membrane; Real time PCR; Transmissible spongiform encephalopathies

Indexed keywords

EPITOPE; POLYVINYLIDENE FLUORIDE; PRION PROTEIN; PRION PROTEIN ANTIBODY; SYNTHETIC DNA;

ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; ARTICLE; CONJUGATION; CONTROLLED STUDY; IMMUNOASSAY; IMMUNOFLUORESCENCE TEST; MOUSE; NONHUMAN; PATHOGENESIS; PRION DISEASE; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN ENGINEERING; QUANTITATIVE ANALYSIS; REAL TIME POLYMERASE CHAIN REACTION; RELIABILITY; RESTRICTION SITE; SCRAPIE; SENSITIVITY ANALYSIS;

ANIMALS; BRAIN CHEMISTRY; IMMUNOASSAY; MICE; MICE, TRANSGENIC; NUCLEOPROTEINS; POLYMERASE CHAIN REACTION; PRIONS; SENSITIVITY AND SPECIFICITY;

EID: 68949149726     PISSN: 01677012     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.mimet.2009.07.001     Document Type: Article

References (15)

1. Aguzzi A., Baumann F., and Bremer J. The prion's elusive reason for being. Annu. Rev. Neurosci. 31 (2008) 439-477
2. Collinge J. Prion diseases of humans and animals: their causes and molecular basis. Annu. Rev. Neurosci. 24 (2001) 519-550
3. Ernst D.R., and Race R.E. Comparative analysis of scrapie agent inactivation methods. J. Virol. Methods 41 (1993) 193-201
4. European Commission. Report on the Monitoring and Testing of Ruminants for the Presence of Transmissible Spongiform Encephalopathy (TSE) in the EU in 2007 (2008). http://ec.europa.eu/food/food/biosafety/bse/annual_report_tse2007_en.pdf http://ec.europa.eu/food/food/biosafety/bse/annual_report_tse2007_en.pdf
5. Fichet G., Comoy E., Duval C., Antloga K., Dehen C., Charbonnier A., et al. Novel methods for disinfection of prion-contaminated medical devices. Lancet 364 (2004) 521-526
6. Fichet G., Comoy E., Dehen C., Challier L., Antloga K., Deslys J.P., et al. Investigations of a prion infectivity assay to evaluate methods of decontamination. J. Microbiol. Methods 70 (2007) 511-518
7. Flechsig E., Hegyi I., Enari M., Schwarz P., Collinge J., and Weissmann C. Transmission of scrapie by steel-surface-bound prions. Mol. Med. 7 (2001) 679-684
8. Gretzschel A., Buschmann A., Langeveld J., and Groschup M.H. Immunological characterization of abnormal prion protein from atypical scrapie cases in sheep using a panel of monoclonal antibodies. J. Gen. Virol. 87 (2006) 3715-3722
9. Johnson C.J., Pedersen J.A., Chappell R.J., McKenzie D., and Aiken J.M. Oral transmissibility of prion disease is enhanced by binding to soil particles. PLoS. Pathog. 3 (2007) e93
10. Klohn P.C., Stoltze L., Flechsig E., Enari M., and Weissmann C. A quantitative, highly sensitive cell-based infectivity assay for mouse scrapie prions. Proc. Natl. Acad. Sci. U. S. A. 100 (2003) 11666-11671
11. Mabbott N.A., and MacPherson G.G. Prions and their lethal journey to the brain. Nat. Rev. Microbiol. 4 (2006) 201-211
12. Prusiner S.B. Prions. Proc. Natl. Acad. Sci. U. S. A. 95 (1998) 13363-13383
13. Saito K., Kobayashi D., Sasaki M., Araake H., Kida T., Yagihashi A., et al. Detection of human serum tumor necrosis factor-alpha in healthy donors, using a highly sensitive immuno-PCR assay. Clin. Chem. 45 (1999) 665-669
14. Taylor D.M., Fraser H., McConnell I., Brown D.A., Brown K.L., Lamza K.A., et al. Decontamination studies with the agents of bovine spongiform encephalopathy and scrapie. Arch. Virol. 139 (1994) 313-326
15. Vilotte J.L., Soulier S., Essalmani R., Stinnakre M.G., Vaiman D., Lepourry L., et al. Markedly increased susceptibility to natural sheep scrapie of transgenic mice expressing ovine prp. J. Virol. 75 (2001) 5977-5984