Evaluation of conjunctival inflammatory status by confocal scanning laser microscopy and conjunctival brush cytology in patients with atopic keratoconjunctivitis (AKC)

Molecular Vision

Volumn 15, Issue , 2009, Pages 1611-1619

  Wakamatsu, Tais Hitomi ^a   Okada, Naoko ^a   Kojima, Takashi ^a   Matsumoto, Yukihiro ^a   Ibrahim, Osama M A ^a   Dogru, Murat ^a,b   Adan, Enrique Sato ^a   Fukagawa, Kazumi ^a   Katakami, Chikako ^c   Tsubota, Kazuo ^a   Shimazaki, Jun ^b   Fujishima, Hiroshi ^d  

^a KEIO UNIVERSITY SCHOOL OF MEDICINE   (Japan)

^b TOKYO DENTAL COLLEGE   (Japan)

^c KOBE KAISEI HOSPITAL   (Japan)

^d International University of Health and Welfare Mita Hospital   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

FLUORESCEIN; ROSE BENGAL;

ADOLESCENT; ADULT; ARTICLE; ATOPIC KERATOCONJUNCTIVITIS; CELL COUNT; CELL DENSITY; CHILD; CLINICAL ARTICLE; CLINICAL TRIAL; CONFOCAL LASER MICROSCOPY; CONJUNCTIVA BRUSH CYTOLOGY; CONTROLLED CLINICAL TRIAL; CONTROLLED STUDY; CORNEA CELL; CYTOLOGY; FEMALE; HUMAN; HUMAN TISSUE; INFLAMMATORY CELL; INTERMETHOD COMPARISON; LACRIMAL FLUID; MALE; PRIORITY JOURNAL; QUANTITATIVE ANALYSIS; SCHOOL CHILD; SCORING SYSTEM; STAINING;

ADOLESCENT; ADULT; CASE-CONTROL STUDIES; CELL COUNT; CHILD; CYTOLOGICAL TECHNIQUES; FEMALE; HUMANS; INFLAMMATION; INJECTIONS; KERATOCONJUNCTIVITIS; MALE; MICROSCOPY, CONFOCAL; MIDDLE AGED; STAINING AND LABELING; SURFACE PROPERTIES; TEARS;

EID: 68949098326     PISSN: None     EISSN: 10900535     Source Type: Journal    
DOI: None     Document Type: Article

References (30)

1. Harrop J, Chinn S, Verlato G, Olivieri M, Norbäck D, Wjst M, Janson C, Zock JP, Leynaert B, Gislason D, Ponzio M, Villani S, Carosso A, Svanes C, Heinrich J, Jarvis D. Eczema, atopy and allergen exposure in adults: a population-based study. Clin Exp Allergy 2007; 37:526-35.
2. Mortz CG, Lauritsen JM, Bindslev-Jensen C, Andersen KE. Prevalence of atopic dermatitis, asthma, allergic rhinitis, and hand and contact dermatitis in adolescents. The Odense Adolescence Cohort Study on Atopic Diseases and Dermatitis. Br J Dermatol 2001; 144:523-32.
3. Bacon AS, Tuft SJ, Metz DM, McGill JI, Buckley RJ, Baddeley S, Lightman SL. The origin of keratopathy in chronic allergic eye disease: a histopathological study. Eye 1993; 7:21-5.
4. Dogru M, Katakami C, Nakagawa N, Tetsumoto K, Yamamoto M. Impression cytology in atopic dermatitis. Ophthalmology 1998; 105:1478-84.
5. Tanaka M, Dogru M, Takano Y, Miyake-Kashima M, Asano-Kato N, Fukagawa K, Tsubota K, Fujishima H. The relation of conjunctival and corneal findings in severe ocular allergies. Cornea 2004; 23:464-7.
6. Takano Y, Fukagawa K, Dogru M, Asano-Kato N, Tsubota K, Fujishima H. Inflammatory cells in brush cytology samples correlate with the severity of corneal lesions in atopic keratoconjunctivitis. Br J Ophthalmol 2004; 88:1504-5.
7. Leonardi A, Brun P, Tavolato M, Plebani M, Abatangelo G, Secchi AG. Tumor necrosis factor-alpha (TNF-alpha) in seasonal allergic conjunctivitis and vernal keratoconjunctivitis. Eur J Ophthalmol 2003; 13:606-10.
8. Lambiase A, Normando EM, Vitiello L, Micera A, Sacchetti M, Perrella E, Racioppi L, Bonini S, Bonini S. Natural killer cells in vernal keratoconjunctivitis. Mol Vis 2007; 13:1562-7.
9. Liang H, Baudouin C, Labbé A, Pauly A, Martin C, Warnet JM, Brignole-Baudouin F. In vivo confocal microscopy and ex vivo flow cytometry: new tools for assessing ocular inflammation applied to rabbit lipopolysaccharide-induced conjunctivitis. Mol Vis 2006; 12:1392-402.
10. Matsuura N, Uchio E, Nakazawa M, Yago T, Matsumoto S, Ohno S, Minami M. Predominance of infiltrating IL-4-producing T cells in conjunctiva of patients with allergic conjunctival disease. Curr Eye Res 2004; 29:235-43.
11. Brignole-Baudouin F, Ott AC, Warnet JM, Baudouin C. Flow cytometry in conjunctival impression cytology: a new tool for exploring ocular surface pathologies. Exp Eye Res 2004; 78:473-81.
12. Tsubota K, Fujihara T, Saito K, Takeuchi T. Conjunctival epithelium expression of HLA-DR in dry eye patients. Ophthalmologica 1999; 213:16-9.
13. Trocme SD, Leiferman KM, George T, Bonini S, Foster CS, Smit EE, Sra SK, Grabowski LR, Dohlman CH. Neutrophil and eosinophil participation in atopic and vernal keratoconjunctivitis. Curr Eye Res 2003; 26:319-25.
14. Kaufman SC, Musch DC, Belin MW, Cohen EJ, Meisler DM, Reinhart WJ, Udell IJ, Van Meter WS. Confocal microscopy. A report by the American Academy of Ophthalmology. Ophthalmology 2004; 111:396-406.
15. Kobayashi A, Yokogawa H, Sugiyama K. In vivo laser confocal microscopy of Bowman's layer of the cornea. Ophthalmology 2006; 113:2203-8.
16. Babu K, Murthy KR. Combined fungal and acanthamoeba keratitis: diagnosis by in vivo confocal microscopy. Eye 2007; 21:271-2.
17. Matsumoto Y, Dogru M, Sato EA, Katono Y, Uchino Y, Shimmura S, Tsubota K. The application of in vivo confocal scanning laser microscopy in the management of Acanthamoeba keratitis. Mol Vis 2007; 13:1319-26.
18. Kobayashi A, Yoshita T, Sugiyama K. In vivo findings of the bulbar/ palpebral conjunctiva and presumed meibomian glands by laser scanning confocal microscopy. Cornea 2005; 24:985-8.
19. Messmer EM, Torres Suárez E, Mackert MI, Zapp DM, Kampik A. In vivo confocal microscopy in blepharitis. Klin Monatsbl Augenheilkd 2005; 222:894-900.
20. De Nicola R, Labbé A, Amar N, Dupas B, Baudouin C. In vivo confocal microscopy and ocular surface disease: anatomical-clinical correlations. J Fr Ophtalmol 2005; 28:691-8.
21. Hu Y, Adan ES, Matsumoto Y, Dogru M, Fukagawa K, Takano Y, Tsubota K, Fujishima H. Conjunctival in vivo confocal scanning laser microscopy in patients with atopic keratoconjunctivitis. Mol Vis 2007; 13:1379-89.
22. Akpek EK, Dart JK, Watson S, Christen W, Dursun D, Yoo S, O'Brien TP, Schein OD, Gottsch JD. A randomized trial of topical cyclosporin 0.05% in topical steroid-resistant atopic keratoconjunctivitis. Ophthalmology 2004; 111:476-82.
23. Junko H. AD classification of fluorescein staining. In: Tano Y, Watanabe N, editors. Practical Ophthalmology. Tokyo: Bunkodo Publishers; 1998. p. 61.
24. Toda I, Tsubota K. Practical double vital staining for ocular surface evaluation. Cornea 1993; 12:366-7.
25. Leibowitz HM, Villazon S. Corneal procedures. In: Leibowitz HM, Waring GO, editors. Corneal Disorders. Philadelphia: WB Saunders; 1998. p. 983-4.
26. Miyoshi T, Fukagawa K, Shimmura S, Fujishima H, Takano Y, Takamura E, Tsubota K, Saito H, Oguchi Y. Interleukin-8 concentrations in conjunctival epithelium brush cytology samples correlate with neutrophil, eosinophil infiltration, and corneal damage. Cornea 2001; 20:743-7.
27. Moy JN, Gleich GJ, Thomas LL. Noncytotoxic activation of neutrophils by eosinophil granule major basic protein. Effect on superoxide anion generation and lysosomal enzyme release. J Immunol 1990; 145:2626-32.
28. Hu Y, Matsumoto Y, Dogru M, Okada N, Igarashi A, Fukagawa K, Tsubota K, Fujishima H. The differences of tear function and ocular surface findings in patients with atopic keratoconjunctivitis and vernal keratoconjunctivitis. Allergy 2007; 62:917-25.
29. Dogru M, Okada N, Asano-Kato N, Igarashi A, Fukagawa K, Shimazaki J, Tsubota K, Fujishima H. Alterations of the ocular surface epithelial mucins 1, 2, 4 and the tear functions in patients with atopic keratoconjunctivitis. Clin Exp Allergy 2006; 36:1556-65.
30. Matsumoto Y, Sato EA, Ibrahim OM, Dogru M, Tsubota K. The application of in vivo laser confocal microscopy to the diagnosis and evaluation of meibomian gland dysfunction. Mol Vis 2008; 14:1263-71.