Study on microassay for screening butyrylcholinesterase inhibitors

Chinese Pharmacological Bulletin

Volumn 25, Issue 5, 2009, Pages 690-692

  Yang, Fu Mei ^a   Sun, Qian Yun ^a  

^a INSTITUTE OF GEOLOGY AND GEOPHYSICS   (China)

Author keywords

Alzheimer's disease; Butyrylcholinesterase; Butyrylcholinesterase inhibitor; Screening model

Indexed keywords

CHINESE DRUG; CHOLINESTERASE; CHOLINESTERASE INHIBITOR; ISO OMPA; PHYSOSTIGMINE; TACRINE;

ALZHEIMER DISEASE; ANIMAL EXPERIMENT; ARTICLE; CONTROLLED STUDY; DRUG SCREENING; ENZYME INHIBITION; IC 50; NONHUMAN; RAT; REACTION OPTIMIZATION; RELIABILITY; SERUM; STATISTICAL ANALYSIS;

EID: 68849128193     PISSN: 10011978     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (8)

1. Greig N H, Lahiri D K, Sambamurti K. Butyrylcholinesterase : an important new target in Alzheimer's disease therapy[ J ]. Int Psychogeriatrics, 2002,14:77-91. (Pubitemid 36206092)
2. Bullock R, Lane R. Executive dyscontrol in dementia, with emphasis on subcortical pathology and the role of butyrylcholinesterase [ J ]. Curr Alzheimer Res, 2007,4(3) :277-293
3. Kamal M A, Klein P, Yu Q S,et al. Kinetics of human serum butyrylcholinesterase and its inhibition by a novel experimental Alzheimer therapeutic, bisnorcymserine [ J ]. J Alzheimers Dis, 2006,10(1);43-51. (Pubitemid 44401365)
4. Ellman G L, Courtney K D, Andres V, Featherstone RM. A new and rapid colorimetric determination of acetylcholinesterase activity [J]. Biochem Pharmacol, 1961,7:88-95.
5. Vogel H G,Vogel W H,Ed. Du G H, Li X J,Zhang Y X,et al. Drug discovery and evaluation-pharmacological assays [ M ]. Beijing: Science Press,2001:423-424
6. Tipton K F. Principles of enzyme assay and kinetic studies[ M ]// Eisenthal R and Danson M J, ed. Enzyme assays : a practical approach. 2nd ed. Oxford:Oxford University Press,2002:1-47.
7. Sun Q Y, Yang F M. Comparative study on microassays for screening acetylcholinesterase inhibitorsf [ J ]. Chin Pharmacol Bull, 2008, 24(10) :1387-92.
8. Hayes D, Mellor G. High throughput screening-considerations for enzyme assays[ M ]// Eisenthal R and Danson M J,ed. Enzyme Assays: a practical approach. 2nd ed. Oxford; Oxford University Press,2002:235-247