PCR detection of four virulence-associated genes of Campylobacter jejuni isolates from Thai broilers and their abilities of adhesion to and invasion of INT-407 cells

Journal of Veterinary Medical Science

Volumn 71, Issue 6, 2009, Pages 839-844

  Chansiripornchai, Niwat ^a   Sasipreeyajan, Jiroj ^a  

^a CHULALONGKORN UNIVERSITY   (Thailand)

Author keywords

Adhesion; Broilers; Campylobacter jejuni; Invasion; Virulence gene

Indexed keywords

BACTERIAL DNA; VIRULENCE FACTOR;

ANIMAL; ANIMAL DISEASE; ARTICLE; BACTERIAL COUNT; BACTERIUM ADHERENCE; BIRD DISEASE; CAMPYLOBACTER JEJUNI; CAMPYLOBACTERIOSIS; CECUM; CHEMISTRY; CHICKEN; FOOD CONTROL; GENETICS; HUMAN; ISOLATION AND PURIFICATION; MICROBIOLOGY; PATHOGENICITY; PHYSIOLOGY; POLYMERASE CHAIN REACTION; THAILAND; VIRULENCE;

ANIMALS; BACTERIAL ADHESION; CAMPYLOBACTER INFECTIONS; CAMPYLOBACTER JEJUNI; CECUM; CHICKENS; COLONY COUNT, MICROBIAL; DNA, BACTERIAL; FOOD MICROBIOLOGY; HUMANS; POLYMERASE CHAIN REACTION; POULTRY DISEASES; THAILAND; VIRULENCE; VIRULENCE FACTORS;

CAMPYLOBACTER; CAMPYLOBACTER JEJUNI;

EID: 68849120234     PISSN: 09167250     EISSN: 13477439     Source Type: Journal    
DOI: 10.1292/jvms.71.839     Document Type: Article

References (44)

1. Achen, M., Morishita, T.Y. and Ley, E.C. 1998. Shedding and colonization of Campylobacter jejuni in broilers from day-ofhatch to slaughter age. Avian Dis. 42: 732-737.
2. Ahmed, I.H., Manning, G., Wassenaar, T.M., Cawthraw, S. and Newell, D.G. 2002. Identification of genetic differences between two Campylobacter jejuni strains with different colonization potentials. Microbiology 148: 1203-1212.
3. Allos, B.M. and Blaser, M.J. 1995. Campylobacter jejuni and the expanding spectrum of related infections. Clin. Infect. Dis. 20: 1092-1099.
4. Bang, D.D., Borck, B., Nielsen, E.M., Scheutz, F., Pedersen, K. and Madsen, M. 2004. Detection of seven virulence and toxin genes of Campylobacter jejuni isolates from Danish turkeys by PCR and cytolethal distending toxin production of the isolates. J. Food Prot. 67: 2171-2177.
5. Bang, D.D., Nielsen, E.M., Scheutz, F., Pedersen, K., Handberg, K. and Madsen, M. 2003. PCR detection of seven virulence and toxin genes of Campylobacter jejuni and Campylobacter coli isolates from Danish pigs and cattle and cytolethal distending toxin production of the isolates. J. Appl. Microbiol. 94: 1003-1014.
6. Biswas, D., Itoh, K. and Sasakawa, C. 2000. Uptake pathways of clinical and healthy animal isolates of Campylobacter jejuni into INT-407 cells. FEMS Immunol. Med. Microbiol. 29: 203-211.
7. Blaser, M.J., Berkowitz, I.D., LaForce, F.M., Cravens, J., Reller, L.B. and Wang, W.L. 1979. Experimental Campylobacter enteritis: clinical and epidemiological features. Annu. Intern. Med. 91: 179-185.
8. Blaser, M.J., Wells, J.G., Feldman, R.A., Pollard, R.A. and Allen, J.R. 1983. Campylobacter enteritis in the United States. A multicenter study. Annu. Intern. Med. 98: 360-365.
9. Cawthraw S.A., Wassenaar, T.M., Ayling, R. and Newell, D.G. 1996. Increased colonization potential of Campylobacter jejuni strain 81116 after passage through chickens and its implication on the rate of transmission within flocks. Epidemiol. Infect. 117: 213-215.
10. Cover, T.L., Perez-Perez, G.I. and Blaser, M.J. 1990. Evaluation of cytotoxic activity in fecal filtrates from patients with Campylobacter jejuni or Campylobacter coli enteritis. FEMS Microbiol. Lett. 58: 301-304.
11. de Melo, M.A. and Pechere, J.C. 1988. Effect of mucin on Campylobacter jejuni association and invasion on HEp-2 cells. Microb. Pathog. 5: 71-76.
12. Deming, M.S., Tauxe, R.V., Blake, P.A., Dixon, S.E., Fowler, B.S., Jones, T.S. Lockamy, E.A., Patton, C.M. and Sikes, R.O. 1987. Campylobacter enteritis at a university: transmission from eating chicken and from cats. Am. J. Epidemiol. 126: 526-534.
13. Grant, C.C., Konkel, M.E., Cieplak, W. Jr. and Tompkins, L.S. 1993. Role of flagella in adherence, internalization, and translocation of Campylobacter jejuni in nonpolarized and polarized epithelial cell cultures. Infect. Immun. 61: 1764-1771.
14. Griffiths, P.L. and Park, R.W. 1990. Campylobacters associated with human diarrhoeal disease. J. Appl. Bacteriol. 69: 281-301.
15. Harris, N.V., Weiss, N.S. and Nolan, C.M. 1986. The role of poultry and meats in the etiology of Campylobacter jejuni/coli enteritis. Am. J. Public Health 76: 407-411.
16. Hu, L. and Kopecko, D.J. 1999. Campylobacter jejuni 81-176 associates with microtubules and dynein during invasion of human intestinal cells. Infect. Immun. 67: 4171-4182.
17. Johnson, W.M. and Lior, H. 1984. Toxins produced by Campylobacter jejuni and Campylobacter coli. Lancet 28: 229-230.
18. Ketley, J.M. 1997. Pathogenesis of enteric infection by Campylobacter. Microbiology 143: 5-21.
19. Konkel, M.E., Corwin, M.D., Joens, L.A. and Cieplak, W. 1992. Factors that influence the interaction of Campylobacter jejuni with cultured mammalian cells. J. Med. Microbiol. 37: 30-37.
20. Konkel, M.E., Garvis, S.G., Tipton, S.L., Anderson, D.E. Jr. and Cieplak, W. Jr. 1997. Identification and molecular cloning of a gene encoding a fibronectin-binding protein (CadF) from Campylobacter jejuni. Mol. Microbiol. 24: 953-963.
21. Konkel, M.E., Gray, S.A., Kim, B.J., Garvis, S.G. and Yoon, J. 1999. Identification of the enteropathogens Campylobacter jejuni and Campylobacter coli based on the cadF virulence gene and its product. J. Clin. Microbiol. 37: 510-517.
22. Konkel, M.E. and Joens, L.A. 1989. Adhesion to and invasion of HEp-2 cells by Campylobacter spp. Infect. Immun. 57: 2984-2990.
23. Konkel, M.E., Kim, B.J., Klena, J.D., Young, C.R. and Ziprin R. 1998. Characterization of the thermal stress response of Campylobacter jejuni. Infect. Immun. 66: 3666-3672.
24. Kulkarni, S.P., Lever, S., Logan, J.M.J., Lawson, A.J., Stanley, J. and Shafi, M.S. 2003. Detection of Campylobacter species: a comparison of culture and polymerase chain reaction based methods. J. Clin. Pathol. 55: 749-753.
25. Kumar, S.S., Malladi, V., Sankaran, K., Haigh, R., Williams, P. and Balakrishnan, A. 2001. Extrusion of actin-positive strands from Hep-2 and Int 407 cells caused by outer membrane preparations of enteropathogenic Escherichia coil and specific attachment of wild type bacteria to the strands. Can. J. Microbiol. 47: 727-734.
26. Lara-Tejero, M. and Galan, J.E. 2001. CdtA, CdtB, and CdtC form a tripartite complex that is required for cytolethal distending toxin activity. Infect. Immun. 69: 4358-4365.
27. Lee, C.A. and Falkow, S. 1990. The ability of Salmonella to enter mammalian cells is affected by bacterial growth state. Proc. Natl. Acad. Sci. U.S.A. 87: 4304-4308.
28. Lior, H. 1984. New, extended biotyping scheme for Campylobacter jejuni, Campylobacter coli, and Campylobacter laridis. J. Clin. Microbiol. 20: 636-640.
29. Mead, P.S., Slutsker, L., Dietz, V., McCaig, L.F., Bresee, J.S., Shapiro, C., Griffin, P.M. and Tauxe, R.V. 1999. Food-related illness and death in the United States. Emerg. Infect. Dis. 5: 607-625.
30. Millar, B.C., Jiru, X., Moore, J.E. and Earle, J.A. 2000. A simple and sensitive method to extracted bacterial, yeast and fungal DNA from blood culture material. J. Microbiol. Methods 42: 139-147.
31. Monteville, M.R., Yoon, J.E. and Konkel, M.E. 2003. Maximal adherence and invasion of INT 407 cells by Campylobacter jejuni requires the CadF outer-membrane protein and microfilament reorganization. Microbiology 149: 153-165.
32. Padungtod, P. and Kaneene, J.B. 2005. Campylobacter in Food Animals and Humans in Northern Thailand. J. Food Prot. 68: 2519-2526.
33. Pang, J.C., Lin, J.S., Tsai, C.C. and Tsen, H.Y. 2006. The presence of major world-wide clones for phage type 4 and 8 Salmonella enterica serovar Enteritidis and the evaluation of their virulence levels by invasiveness assays in vitro and in vivo. FEMS Microbiol. Lett. 263: 148-154.
34. Rivera-Amill, V., Kim, B.J., Seshu, J. and Konkel, M.E. 2001. Secretion of the virulence-associated Campylobacter invasion antigens from Campylobacter jejuni requires a stimulatory signal. J. Infect. Dis. 183: 1607-1616.
35. Ruiz-Palacios, G.M., Escamilla, E. and Torres, N. 1981. Experimental Campylobacter diarrhea in chickens. Infect. Immun. 34: 250-255.
36. Shanker, S., Lee, A. and Sorrell, T.C. 1990. Horizontal transmission of Campylobacter jejuni amongst broiler chicks: experimental studies. Epidemiol. Infect. 104: 101-110.
37. Smibert, R.M. 1984. Genus Campylobacter Sebald and Veron 1963, 907AL. pp. 111-118. In: Bergey's Manual of Systematic Bacteriology, vol. 1 (Krieg, N.R. ed.), Williams and Wilkins, Baltimore, Md.
38. Stern, N.J. and Pretanik, S. 2006. Counts of Campylobacter spp. on U.S. broiler carcasses. J. Food Prot. 69: 1034-1039.
39. van Spreeuwel, J.P., Duursma, G.C., Meijer, C.J., Bax, R., Rosekrans, P.C. and Lindeman, J. 1985. Campylobacter colitis: histological immunohistochemical and ultrastructural findings. Gut 26: 945-951.
40. Wang, G., Clark, C.G., Taylor, T.M., Pucknell, C., Barton, C., Price, L., Woodward, D.L. and Rodgers, F.G. 2002. Colony multiplex PCR assay for identification and differentiation of Campylobacter jejuni, C. coli, C. lari, C. upsaliensis, and C. fetus subsp. fetus. J. Clin. Microbiol. 40: 4744-4747.
41. Wooldridge, K.G. and Ketley, J.M. 1997. Campylobacter-host cell interactions. Trends Microbiol. 5: 96-102.
42. Zheng, J., Meng, J., Zhao, S., Singh, R. and Song, W. 2006. Adherence to and Invasion of Human Intestinal Epithelial Cells by Campylobacter jejuni and Campylobacter coli Isolates from Retail Meat Products. J. Food Prot. 69: 768-774.
43. Ziprin, R.L., Young, C.R., Byrd, J.A., Stanker, L.H., Hume, M.E., Gray, S.A., Kim, B.J. and Konkel, M.E. 2001. Role of Campylobacter jejuni potential virulence genes in cecal colonization. Avian Dis. 45: 549-557.
44. Ziprin, R.L., Young, C.R., Stanker, L.H., Hume, M.E. and Konkel, M.E. 1999. The absence of cecal colonization of chicks by a mutant of Campylobacter jejuni not expressing bacterial fibronectin-binding protein. Avian Dis. 43: 586-589.