Production of N-acetyl-D-neuraminic acid using two sequential enzymes overexpressed as double-tagged fusion proteins

BMC Biotechnology

Volumn 9, Issue , 2009, Pages

  Wang, Tzu Hsien ^a   Chen, Ying Yin ^a   Pan, Hsin Hung ^a   Wang, Feng Pao ^a   Cheng, Chung Hsien ^a   Lee, Wen Chien ^a  

^a NATIONAL CHUNG CHENG UNIVERSITY   (Taiwan)

Author keywords

[No Author keywords available]

Indexed keywords

ALDOLASES; ARGININE RESIDUE; ASPARTATE RESIDUE; C-TERMINUS; CRUDE PROTEINS; DIRECT CAPTURE; ENZYMATIC ACTIVITIES; ENZYMATIC PRODUCTION; ENZYME REACTION; FUSION PROTEINS; GLUTATHIONE-S-TRANSFERASE; IONIC EXCHANGE; N-ACETYL-D-GLUCOSAMINE; ONE POT; SIALIC ACIDS; SINGLE-STEP; TAGGED GENES; TAGGED PROTEINS;

ACIDS; AMINO ACIDS; CARBOXYLIC ACIDS; CATALYSTS; DYES; ENZYME IMMOBILIZATION; ESCHERICHIA COLI; GEL PERMEATION CHROMATOGRAPHY; ION EXCHANGE; RESINS;

ENZYMES;

ARGININE; ASPARTIC ACID; HYBRID PROTEIN; N ACETYLGLUCOSAMINE; N ACETYLNEURAMINIC ACID; PROTEIN TAG; SIALIC ACID; CARRIER PROTEIN; EPIMERASE; IMMOBILIZED ENZYME; LYASE; N ACETYLNEURAMINATE LYASE; N ACYL D GLUCOSAMINE 2 EPIMERASE; N-ACETYLNEURAMINATE LYASE; N-ACYL-D-GLUCOSAMINE 2-EPIMERASE;

AMINO ACID SYNTHESIS; AMINO TERMINAL SEQUENCE; ANION EXCHANGE; ARTICLE; BINDING AFFINITY; CARBOXY TERMINAL SEQUENCE; CATION EXCHANGE; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME BINDING; ENZYME IMMOBILIZATION; ENZYME ISOLATION; ENZYME MECHANISM; ENZYME SUBSTRATE; ESCHERICHIA COLI; GENE OVEREXPRESSION; NONHUMAN; PROTEIN EXPRESSION; BIOSYNTHESIS; ENZYMOLOGY; GENETICS; METABOLISM; SYNECHOCYSTIS;

ESCHERICHIA COLI;

CARBOHYDRATE EPIMERASES; CARRIER PROTEINS; ENZYMES, IMMOBILIZED; ESCHERICHIA COLI; N-ACETYLNEURAMINIC ACID; OXO-ACID-LYASES; RECOMBINANT FUSION PROTEINS; SYNECHOCYSTIS;

EID: 68849112214     PISSN: None     EISSN: 14726750     Source Type: Journal    
DOI: 10.1186/1472-6750-9-63     Document Type: Article

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