Cryopreservation of in vitro apices of strawberry (Fragaria x ananassa Duch.) by a new encapsulation-dehydration method

Acta Horticulturae

Volumn 649, Issue , 2004, Pages 115-118

  Clavero Ramirez ^a   Lopez Aranda J M ^a   Galvez J ^a   Soria, C ^a   Sanchez Sevilla J F ^a   Medina, J J ^b   Bartual, R ^c   Gonzalez Benito M E ^d  

^a IFAPA CENTRO EL TORUÑO   (Spain)

^b CAP JA ^*  (Spain)

^c INSTITUTO VALENCIANO DE INVESTIGACIONES AGRARIAS IVIA   (Spain)

^d UNIVERSIDAD POLITÉCNICA DE MADRID   (Spain)

Author keywords

Cold hardening; Germplasm; Liquid nitrogen; Long term conservation; Meristems; Silica gel

Indexed keywords

EID: 68849108951     PISSN: 05677572     EISSN: None     Source Type: Book Series    
DOI: None     Document Type: Conference Paper

References (8)

1. Benson, E.E., Lyncland, T. and Stacey, G.N. 1998. Advances in plant cryoporeservation technology: current applications in crop plant biotechnology. AgBiotechNews and Information 10: 133-142.
2. Clavero-Ramírez, I., López-Aranda, J.M., Gálvez-Farfán, J. and González-Benito, M.E. 2001. Crioconservación de ápices de fresa (Fragaria x ananassa Duch.) mediante encapsulación-desecación. Actas de Horticultura 28: 352-359.
3. Engelmann, F. 2000. Importance of cryopreservation for the conservation of plant genetic resources. p.8-20. In: F. Engelmann and H. Takagi (eds), Cryopreservation of Tropical Plant Germplasm, IPGRI, Rome.
4. Fabre, J. and Dereuddre, J. 1990. Encapsulation-dehydration: a new approach to cryopreservation of Solanum shoots tips. Cryo-Lett. 11: 413-426.
5. González-Benito, M.E. and Pérez, C. 1997. Cryopreservation of nodal explants of an endangered plant species (Centaurium rigualii Esteve) using the encapsultion-dehydration method. Biodiversity and Conservation 6: 583-590.
6. Hirai, D., Shirai, K., Shirai, S. and Sakai, A. 1998. Cryopreservation of in vitro-grown meristems of strawberry (Fragaria x ananassa Duch.). by encapsulation-vitrification. Euphytica 101: 109-115.
7. Sakai, A., Matsumoto, T., Hirai D. and Niino, T. 2000. Newly development encapsulation-dehydration protocol for plant cryopreservation. Cryo-Lett. 21: 53-62.
8. López-Aranda, J.M., Pliego-Alfaro, F., López-Navidad, I. and Barceló-Muñoz, M. 1994. Micropropagación of strawberry (Fragaria x ananassa Duch.). Effect of mineral salts, benzyladenine levels and number of subcultures on the in vitro and fields behaviour of the obtained microplants and the fruiting capacity of their progeny. J. Hort. Sci. 69: 625-637.