Development and validation of cytokine quantitative, real time RT-PCR assays for characterization of Asian elephant immune responses

Veterinary Immunology and Immunopathology

Volumn 131, Issue 1-2, 2009, Pages 73-78

  Landolfi, Jennifer A ^a   Schultz, Stacy A ^a   Mikota, Susan K ^b   Terio, Karen A ^a  

^a LOYOLA UNIVERSITY MEDICAL CENTER   (United States)

^b Elephant Care International   (United States)

Author keywords

Asian elephant (Elephas maximus); Cytokine; Immune system; Infectious disease; RT PCR

Indexed keywords

BETA ACTIN; DNA; GAMMA INTERFERON; GLYCERALDEHYDE 3 PHOSPHATE DEHYDROGENASE; INTERLEUKIN 10; INTERLEUKIN 12; INTERLEUKIN 2; INTERLEUKIN 4; MESSENGER RNA; TRANSFORMING GROWTH FACTOR BETA; TUMOR NECROSIS FACTOR ALPHA;

ANIMAL CELL; ARTICLE; ASIA; CONTROLLED STUDY; ELEPHANT; GENE EXPRESSION; IMMUNE RESPONSE; INFECTION RESISTANCE; INFECTION SENSITIVITY; MEDICAL RESEARCH; MOLECULAR PROBE; NONHUMAN; NUCLEOTIDE SEQUENCE; PATHOGENESIS; QUANTITATIVE ANALYSIS; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RNA SEQUENCE; SENSITIVITY AND SPECIFICITY; SEQUENCE ANALYSIS; VALIDATION STUDY;

ANIMALS; CYTOKINES; ELEPHANTS; FEMALE; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA, MESSENGER;

ELEPHAS MAXIMUS;

EID: 68549126981     PISSN: 01652427     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.vetimm.2009.03.012     Document Type: Article

References (16)

1. Abbas A.K., and Lichtman A.H. Effector Mechanisms of Immune Responses in Cellular and Molecular Immunity (2003), Saunders, Philadelphia, Pennsylvania pp. 241-345
2. Dallman M.J., Montgomery R.A., Larsen C.P., Wanders A., and Wells A.F. Cytokine gene expression: analysis using Northern blotting, polymerase chain reaction, and in situ hybridization. Immunol. Rev. 119 (1991) 163-179
3. Dean G.A., Higgins J., LaVoy A., Fan Z., and Pedersen N.C. Measurement of feline cytokine gene expression by quantitative-competitive RT-PCR. Vet. Immunol. Immunopath. 63 (1998) 73-82
4. De Jong W.W., van Dijk M.A., Poux C., Kappe G., van Reede T., and Madson O. Indels in protein-coding sequences of Euarchontoglires constrain the rooting of the eutherian tree. Mol. Phylogenet. Evol. 28 2 (2003) 328-340
5. Doherty T.M., Demissie A., Menzies D., Andersen P., Rook G., and Zumla A. Effect of sample handling on analysis of cytokine responses to Mycobacterium tuberculosis in clinical samples using ELISA, ELISPOT, and quantitative PCR. J. Immunol. Methods 298 (2005) 129-141
6. Hayden R.T. In vitro nucleic acid amplification techniques. In: Persing D.H., Tenover F.C., Versalovic J., Tang Y., Unger E.R., Relman D.A., and White T.J. (Eds). Molecular Microbiology Diagnostic Principles and Practice (2004), American Society for Microbiology Press, Washington, D.C. 43-64
7. Kaufmann S.H., Cole S.T., Mizrah V., Rubin E., and Nathan C. Mycobacterium tuberculosis and the host response. J. Exp. Med. 201 11 (2005) 1693-1697
8. Kidd P. Th1/Th2 balance: the hypothesis, its limitations, and implications for health and disease. Altern. Med. Rev. 8 3 (2003) 223-246
9. Leutenegger C.M., Mislin C.N., Sigrist B., Ehrengruber M.U., Hofmann-Lehmann R., and Lutz H. Quantitative real-time PCR for the measurement of feline cytokine mRNA. Vet. Immunol. Immunopath. 71 (1999) 291-305
10. Mikota S.K., Peddie L., Peddie J., Isaza R., Dunker F., West G., Lindsay W., Larsen R.S., Salman M.D., Chatterjee D., Payeur J., Whipple D., Thoen C., Davis D.S., Sedgwick C., Montali R.J., Ziccardi M., and Maslow J. Epidemiology and diagnosis of Mycobacterium tuberculosis in captive Asian elephants (Elephas maximus). J. Zool. Wildl. Med. 32 1 (2001) 1-16
11. Nowak R.M. Walker's Mammals of the World. 6 ed. vol. II (1999), Johns Hopkins University Press, Baltimore pp. 998
12. Overbergh I., Giulietti A., Valckx D., Decallonne B., Bouillon R., and Mathieu C. The use of real-time reverse transcriptase PCR for the quantification of cytokine gene expression. J. Biomol. Technol. 14 (2003) 33-43
13. Richman L.K., Montali R.J., Cambre R.C., Schmitt D., Hardy D., Hildbrandt T., Bengis R.G., Hamzeh F.M., Shahkolahi A., and Hayward G.S. Clinical and pathological findings of a newly recognized disease of elephants caused by endotheliotropic herpesviruses. J. Wildl. Dis. 36 1 (2000) 1-12
14. Schmitt D.L. Proboscidae (elephants). In: Fowler M.E., and Miller R.E. (Eds). Zoo and Wild Animal Medicine. 5 ed. (2003), Saunders, St. Louis 545-547
15. Sreekumar E., Janki M.B.V., Arathy D.S., Hariharan R., Avinash D., Premraj C., and Rasool T.J. Molecular characterization and expression of interferon-γ of Asian elephant (Elephas maximus). Vet. Immun. Immunopath. 118 (2007) 75-83
16. Wittwer C.T., and Kusukawa N. Real time PCR. In: Persing D.H., Tenover F.C., Versalovic J., Tang Y., Unger E.R., Relman D.A., and White T.J. (Eds). Molecular Microbiology Diagnostic Principles and Practice (2004), American Society for Microbiology Press, Washington, D.C. 71-83