Detection and quantification of hepatitis C virus (HCV) by MultiCode-RTX real-time PCR targeting the HCV 3′ untranslated region

Journal of Clinical Microbiology

Volumn 47, Issue 8, 2009, Pages 2635-2638

  Mulligan, Elizabeth K ^a   Germer, J J ^b   Arens, Max Q ^c   D'Amore, Krista L ^d   Di Bisceglie, Adrian ^e   Ledeboer, Nathan A ^d,f   Moser, Michael J ^a   Newman, Andrew C ^a   O'Guin, Andy K ^g   Olivo, Paul D ^g   Podzorski, Diane S ^f   Vaughan, Kimberly A ^f   Yao, Joseph D ^b   Elagin, Slava A ^a   Johnson, Scott C ^a  

^a EraGen Biosciences   (United States)

^b MAYO CLINIC   (United States)

^c WASHINGTON UNIVERSITY SCHOOL OF MEDICINE   (United States)

^d MEDICAL COLLEGE OF WISCONSIN   (United States)

^e SAINT LOUIS UNIVERSITY SCHOOL OF MEDICINE   (United States)

^f DYNACARE LABORATORIES   (Canada)

^g Apath LLC   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

VIRUS RNA;

3' UNTRANSLATED REGION; ARTICLE; CONTROLLED STUDY; CORRELATION ANALYSIS; GENOTYPE; HEPATITIS C VIRUS; MEDICAL TECHNOLOGY; NONHUMAN; PRIORITY JOURNAL; QUANTITATIVE ASSAY; REAL TIME POLYMERASE CHAIN REACTION; VIRAL GENETICS; VIRUS DETECTION;

3' UNTRANSLATED REGIONS; HEPACIVIRUS; HEPATITIS C; HUMANS; PLASMA; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA, VIRAL; SENSITIVITY AND SPECIFICITY; SERUM; VIRAL LOAD;

HEPATITIS C VIRUS;

EID: 68549083456     PISSN: 00951137     EISSN: None     Source Type: Journal    
DOI: 10.1128/JCM.02170-08     Document Type: Article

References (18)

1. Barbeau, J. M., J. Goforth, A. M. Caliendo, and F. S. Nolte. 2004. Performance characteristics of a quantitative TaqMan hepatitis C virus RNA analyte-specific reagent. J. Clin. Microbiol. 42:3739-3746.
2. Caliendo, A. M., A. Valsamakis, Y. Zhou, B. Yen-Lieberman, J. Andersen, S. Young, A. Ferreira-Gonzalez, G. J. Tsongalis, R. Pyles, J. W. Bremer, and N. S. Lurain. 2006. Multilaboratory comparison of hepatitis C virus viral load assays. J. Clin. Microbiol. 44:1726-1732.
3. Choo, Q. L., K. H. Richman, J. H. Han, K. Berger, C. Lee, C. Dong, C. Gallegos, D. Coit, R. Medina-Selby, P. J. Barr, et al. 1991. Genetic organization and diversity of the hepatitis C virus. Proc. Natl. Acad. Sci. USA 88:2451-2455.
4. Colucci, G., J. Ferguson, C. Harkleroad, S. Lee, D. Romo, S. Soviero, J. Thompson, M. Velez, A. Wang, Y. Miyahara, S. Young, and C. Sarrazin. 2007. Improved COBAS TaqMan hepatitis C virus test (version 2.0) for use with the High Pure system: enhanced genotype inclusivity and performance characteristics in a multisite study. J. Clin. Microbiol. 45:3595-3600.
5. Ferreira-Gonzalez, A., M. L. Shiffman, D. H. Lofland, M. R. Langley, D. W. Gainey, and C. T. Garrett. 1996. Assessing clinical utility of hepatitis C virus quantitative RT-PCR data: implications for identification of responders among alpha-interferon-treated patients. Mol. Diagn. 1:109-120.
6. Forman, M. S., and A. Valsamakis. 2004. Verification of an assay for quantification of hepatitis C virus RNA by use of an analyte-specific reagent and two different extraction methods. J. Clin. Microbiol. 42:3581-3588.
7. Houghton, M., Q. L. Choo, G. Kuo, A. J. Weiner, J. Han, M. S. Urdea, B. D. Irvine, and J. A. Kolberg. January 1998. Methods for detecting hepatitis C virus using polynucleotides specific for same. U.S. patent 5,712,088.
8. Kolykhalov, A. A., S. M. Feinstone, and C. M. Rice. 1996. Identification of a highly conserved sequence element at the 3′ terminus of hepatitis C virus genome RNA. J. Virol. 70:3363-3371.
9. Konnick, E. Q., S. M. Williams, E. R. Ashwood, and D. R. Hillyard. 2005. Evaluation of the COBAS hepatitis C virus (HCV) TaqMan analyte-specific reagent assay and comparison to the COBAS Amplicor HCV Monitor V2.0 and Versant HCV bDNA 3.0 assays. J. Clin. Microbiol. 43:2133-2140.
10. Majid, A. M., and D. R. Gretch. 2002. Current and future hepatitis C virus diagnostic testing: problems and advancements. Microbes Infect. 4:1227-1236.
11. Moser, M. J., D. R. Christensen, D. Norwood, and J. R. Prudent. 2006. Multiplexed detection of anthrax-related toxin genes. J. Mol. Diagn. 8:89-96.
12. National Institutes of Health. 2006. National Institutes of Health Consensus Development Conference statement. Management of hepatitis C: 2002-2004. National Institutes of Health, Bethesda, MD.
13. Pawlotsky, J. M. 2002. Molecular diagnosis of viral hepatitis. Gastroenterology 122:1554-1568.
14. Sábato, M. F., M. L. Shiffman, M. R. Langley, D. S. Wilkinson, and A. Ferreira-Gonzalez. 2007. Comparison of performance characteristics of three real-time reverse transcription-PCR test systems for detection and quantification of hepatitis C virus. J. Clin. Microbiol. 45:2529-2536.
15. Selvaraju, S. B., M. Wurst, R. T. Horvat, and R. Selvarangan. 2009. Evaluation of three analyte-specific reagents for detection and typing of herpes simplex virus in cerebrospinal fluid. Diagn. Microbiol. Infect. Dis. 63:286-291.
16. Sherrill, C. B., D. J. Marshall, M. J. Moser, C. A. Larsen, L. Daude-Snow, S. Jurczyk, G. Shapiro, and J. R. Prudent. 2004. Nucleic acid analysis using an expanded genetic alphabet to quench fluorescence. J. Am. Chem. Soc. 126:4550-4556.
17. Svarovskaia, E. S., M. J. Moser, A. S. Bae, J. R. Prudent, M. D. Miller, and K. Borroto-Esoda. 2006. MultiCode-RTx real-time PCR system for detection of subpopulations of K65R human immunodeficiency virus type 1 reverse transcriptase mutant viruses in clinical samples. J. Clin. Microbiol. 44:4237-4241.
18. Young, K. K., R. M. Resnick, and T. W. Myers. 1993. Detection of hepatitis C virus RNA by a combined reverse transcription-polymerase chain reaction assay. J. Clin. Microbiol. 31:882-886.