A protocol for preparing GFP-labeled neurons previously imaged in vivo and in slice preparations for light and electron microscopic analysis

Nature Protocols

Volumn 4, Issue 8, 2009, Pages 1145-1156

  Knott, Graham W ^a   Holtmaat, Anthony ^b   Trachtenberg, Joshua T ^c   Svoboda, Karel ^d   Welker, Egbert ^e  

^a EPFL   (Switzerland)

^b UNIVERSITY OF GENEVA   (Switzerland)

^c UNIVERSITY OF CALIFORNIA   (United States)

^d HOWARD HUGHES MEDICAL INSTITUTE   (United States)

^e UNIVERSITY OF LAUSANNE   (Switzerland)

Author keywords

[No Author keywords available]

Indexed keywords

EPOXY RESIN; GREEN FLUORESCENT PROTEIN;

ARTICLE; BRAIN SLICE; BRAIN SYNAPTOSOME; CELL LABELING; CELL ULTRASTRUCTURE; CELLULAR DISTRIBUTION; DENDRITE; ELECTRON MICROSCOPY; EXPERIMENTAL DESIGN; IMAGE ANALYSIS; IMAGE RECONSTRUCTION; IMMUNOCYTOCHEMISTRY; IMMUNOLOCALIZATION; IN VIVO STUDY; LASER MICROSCOPY; MICROSCOPIC ANATOMY; MICROSCOPY; NERVE CELL NETWORK; NERVE CELL PLASTICITY; NERVE FIBER; NERVE FIBER TRANSECTION; NEUROANATOMY; NEUROIMAGING; NEUROPIL; NONHUMAN; PERMEABILITY; PRIORITY JOURNAL; THREE DIMENSIONAL IMAGING; TISSUE FIXATION; TISSUE PREPARATION; TISSUE SLICE;

ANIMALS; AXONS; DENDRITES; GREEN FLUORESCENT PROTEINS; IMAGING, THREE-DIMENSIONAL; IMMUNOHISTOCHEMISTRY; MICE; MICROSCOPY, CONFOCAL; MICROSCOPY, ELECTRON, TRANSMISSION; MICROTOMY; NEURONS; SOFTWARE; STAINING AND LABELING; SYNAPSES;

EID: 68449104741     PISSN: 17542189     EISSN: 17502799     Source Type: Journal    
DOI: 10.1038/nprot.2009.114     Document Type: Article

References (12)

1. Zito, K., Knott, G., Shepherd, G.M., Shenolikar, S. & Svoboda, K. Induction of spine growth and synapse formation by regulation of the spine actin cytoskeleton. Neuron 44, 321-334 (2004).
2. Zito, K., Scheuss, V., Knott, G., Hill, T. & Svoboda, K. Rapid functional maturation of nascent dendritic spines. Neuron 61 247-258 (2009).
3. Holtmaat, A.J. et al. Transient and persistent dendritic spines in the neocortex in vivo. Neuron 45, 279-291 (2005).
4. Trachtenberg, J.T. et al. Long-term in vivo imaging of experience-dependent synaptic plasticity in adult cortex. Nature 420, 788-794 (2002).
5. Knott, G.W., Holtmaat, A., Wilbrecht, L., Welker, E. & Svoboda, K. Spine growth precedes synapse formation in the adult neocortex in vivo. Nat. Neurosci. 9, 1117-1124 (2006).
6. De Paola, V. et al. Cell type-specific structural plasticity of axonal branches and boutons in the adult neocortex. Neuron 49 861-875 (2006).
7. Zuo, Y., Yang, G., Kwon, E. & Gan, W.B. Long-term sensory deprivation prevents dendritic spine loss in primary somatosensory cortex. Nature 436, 261-265 (2005).
8. Holtmaat, A. et al. Long-term, high-resolution imaging in the mouse neocortex through a chronic cranial window. Nat. Protoc. 4, 1128-1144 (2009).
9. Holtmaat, A., Wilbrecht, L., Knott, G.W., Welker, E. & Svoboda, K. Experience-dependent and cell-type-specific spine growth in the neocortex. Nature 441, 979-983 (2006).
10. Feng, G. et al. Imaging neuronal subsets in transgenic mice expressing multiple spectral variants of GFP. Neuron 28, 41-51 (2000).
11. Hoffpauir, B.K., Pope, B.A. & Spirou, G.A. Serial sectioning and electron microscopy of large tissue volumes for 3D analysis and reconstruction: A case study of the calyx of Held. Nat. Protoc. 2, 9-22 (2007).
12. Harris, K.M. et al. Uniform serial sectioning for transmission electron microscopy. J. Neurosci. 26, 12101-12103 (2006).