Incorporation of thymidine analogs for studying replication kinetics in fission yeast

Methods in Molecular Biology

Volumn 521, Issue , 2009, Pages 509-515

  Rhind, Nicholas ^a  

^a NONE

Author keywords

BrdU; CldU; DNA replication; Fission yeast; hENT1; IdU; Schizosaccharomyces pombe; Thymidine; Thymidine kinase

Indexed keywords

FUNGI; SCHIZOSACCHAROMYCES POMBE; SCHIZOSACCHAROMYCETACEAE;

BROXURIDINE; DRUG DERIVATIVE; FUNGAL DNA; THYMIDINE;

ARTICLE; BIOSYNTHESIS; DENSITY GRADIENT CENTRIFUGATION; DNA REPLICATION; KINETICS; METABOLISM; METHODOLOGY; MYCOLOGY; SCHIZOSACCHAROMYCES;

BROMODEOXYURIDINE; CENTRIFUGATION, DENSITY GRADIENT; DNA REPLICATION; DNA, FUNGAL; KINETICS; MYCOLOGY; SCHIZOSACCHAROMYCES; THYMIDINE;

EID: 68349140142     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-60327-815-7_29     Document Type: Article

References (5)

1. Sivakumar, S., Porter-Goff, M., Patel, P. K., Benoit, K. and Rhind, N. (2004) In vivo labeling of fission yeast DNA with thymi-dine and thymidine analogs. Methods 33, 213-219. (Pubitemid 38648842)
2. Hodson, J. A., Bailis, J. M. and Forsburg, S. L. (2003) Efficient labeling of fission yeast Schizosaccharomyces pombe with thymidine and BUdR. Nucleic Acids Res 31, e134.
3. McCarroll, R. M. and Fangman, W. L. (1988) Time of replication of yeast centromeres and telomeres. Cell 54, 505-513.
4. Tercero, J. A. and Diffley, J. F. (2001) Regulation of DNA replication fork progression through damaged DNA by the Mec1/Rad53 checkpoint. Nature 412, 553-557. (Pubitemid 32743835)
5. Forsburg, S. L. and Rhind, N. (2006) Basic methods for fission yeast. Yeast 23, 173-183.