Comet fluorescence in situ hybridization (Comet-FISH): Detection of DNA damage

Cold Spring Harbor Protocols

Volumn 4, Issue 5, 2009, Pages

  Schlörmann, Wiebke ^a   Glei, Michael ^a  

^a FRIEDRICH SCHILLER UNIVERSITY JENA   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

DNA; REAGENT;

ARTICLE; COMET ASSAY; DNA DAMAGE; DNA HYBRIDIZATION; DNA REPAIR; DNA SEQUENCE; DNA STRAND BREAKAGE; FLUORESCENCE IN SITU HYBRIDIZATION; QUANTITATIVE ANALYSIS;

EID: 68349117034     PISSN: None     EISSN: 15596095     Source Type: Journal    
DOI: 10.1101/pdb.prot5220     Document Type: Article

References (12)

1. Anderson, D., Yu, T.W., and Browne, M.A. 1997. The use of the same image analysis system to detect genetic damage in human lymphocytes treated with doxorubicin in the Comet and fluorescence in situ hybridisation (FISH) assays. Mutat. Res. 390: 69-77.
2. Bock, C., Rapp, A., Dittmar, H., Monajembashi, S., and Greulich, K.O. 1999. Localisation of specific sequences and DNA single strand breaks in individual UV-A irradiated human lymphocytes by COMET FISH. Prog. Biomed. Optics 3568: 207-217.
3. Glei, M., Hovhannisyan, G., and Pool-Zobel, B.L. 2009. Use of Comet-FISH in the study of DNA damage and repair: Review. Mutat. Res. 681: 33-43.
4. Horvathova, E., Dusinska, M., Shaposhnikov, S., and Collins, A.R. 2004. DNA damage and repair measured in different genomic regions using the comet assay with fluorescent in situ hybridization. Mutagenesis 19: 269-276.
5. Knöbel, Y., Glei, M., Weise, A., Osswald, K., Schäferhenrich, A., Richter, K.K., Claussen, U., and Pool-Zobel, B.L. 2006. Uranyl nitrilotriacetate, a stabilized salt of uranium, is genotoxic in nontransformed human colon cells and in the human colon adenoma cell line LT97. Toxicol. Sci. 93: 286-297.
6. Knöbel, Y., Weise, A., Glei, M., Sendt, W., Claussen, U., and Pool-Zobel, B.L. 2007. Ferric iron is genotoxic in nontransformed and preneoplastic human colon cells. Food Chem. Toxicol. 45: 804-811.
7. McKelvey-Martin, V.J, Ho, E.T., McKeown, S.R., Johnston, S.R., McCarthy, P.J., Rajab, N.F., and Downes, C.S. 1998. Emerging applications of the single cell gel electrophoresis (Comet) assay. I. Management of invasive transitional cell human bladder carcinoma. II. Fluorescent in situ hybridization Comets for the identification of damaged and repaired DNA sequences in individual cells. Mutagenesis 13: 1-8.
8. Ooi, F.A. 2001. Oncogene amplification detection by fluorescence in situ hybridization (FISH). Acta Histochem. Cytochem. 34: 391-397.
9. Rapp, A., Bock, C., Dittmar, H., and Greulich, K.O. 2000. UV-A breakage sensitivity of human chromosomes as measured by COMET-FISH depends on gene density and not on the chromosome size. J. Photochem. Photobiol. B 56: 109-117.
10. Rapp, A., Hausmann, M., and Greulich, K.O. 2005. The comet-FISH technique: A tool for detection of specific DNA damage and repair. Methods Mol. Biol. 291: 107-119.
11. Schäferhenrich, A., Beyer-Sehlmeyer, G., Festag, G., Kuechler, A., Haag, N., Weise, A., Liehr, T., Claussen, U., Marian, B., Sendt, W., et al. 2003. Human adenoma cells are highly susceptible to the genotoxic action of 4-hydroxy-2-nonenal. Mutat. Res. 526: 19-32.
12. Wollowski, I., Ji, S.T., Bakalinsky, A.T., Neudecker, C., and Pool-Zobel, B.L. 1999. Bacteria used for the production of yogurt inactivate carcinogens and prevent DNA damage in the colon of rats. J. Nutr. 129: 77-82.