Whole-mount in situ hybridization of bichir (Polypterus) embryos

Cold Spring Harbor Protocols

Volumn 4, Issue 5, 2009, Pages

  Takeuchi, Masaki ^a   Okabe, Masataka ^a,b   Aizawa, Shinichi ^a  

^a Center for Developmental Biology   (Japan)

^b JIKEI UNIVERSITY SCHOOL OF MEDICINE   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

METHANOL; PROTEINASE K;

ANTIGEN ANTIBODY REACTION; ARTICLE; BICHIR; CULTURE MEDIUM; EMBRYO DEVELOPMENT; FISH; IN SITU HYBRIDIZATION; NONHUMAN; TISSUE FIXATION;

POLYPTERUS;

EID: 68349099923     PISSN: None     EISSN: 15596095     Source Type: Journal    
DOI: 10.1101/pdb.prot5158     Document Type: Article

References (5)

1. Harland, R.M. 1991. In situ hybridization: An improved whole-mount method for Xenopus embryos. Methods Cell Biol. 36: 685-695.
2. Monsoro-Burq, A.H. 2007. A rapid protocol for whole-mount in situ hybridization on Xenopus embryos. Cold Spring Harb. Protoc. doi: 10.1101/pdb.prot4809.
3. Sive, H.L., Grainger, R.M., and Harland, R.M. 2007. Synthesis and purification of digoxigenin-labeled RNA probes for in situ hybridization. Cold Spring Harb. Protoc. doi: 10.1101/ pdb.prot4778.
4. Takeuchi, M., Okabe, M., and Aizawa, S. 2009a. The genus Polypterus(bichirs): A fish group diverged at the stem of ray-finned fishes (Actinopterygii). Cold Spring Harb. Protoc. (this issue), doi: 10.1101/pdb.emo117.
5. Takeuchi, M., Okabe, M., and Aizawa, S. 2009b. Microinjection of bichir (Polypterus) embryos. Cold Spring Harb. Protoc. (this issue), doi: 10.1101/pdb.prot5157.