Validation of the use of doubly labeled water for estimating metabolic rate in the green turtle (Chelonia mydas L.): A word of caution

Journal of Experimental Biology

Volumn 212, Issue 16, 2009, Pages 2635-2644

  Jones, T Todd ^a   Hastings, Mervin D ^a,b   Bostrom, Brian L ^a   Andrews, Russel D ^c   Jones, David R ^a  

^a UNIVERSITY OF BRITISH COLUMBIA   (Canada)

^b Government of the British Virgin Islands ^*  (Virgin Islands (British))

^c UNIVERSITY OF ALASKA FAIRBANKS   (United States)

Author keywords

Deuterium; Doubly labeled water; Fasting; Marine turtle; Metabolic rate; Oxygen consumption; Oxygen 18; RER; Respirometry; Total body water; Validation; Water turnover

Indexed keywords

DEUTERIUM; OXYGEN; SEA WATER;

ANIMAL; ARTICLE; BASAL METABOLIC RATE; BODY COMPOSITION; BODY WATER; ECOSYSTEM; ELECTROLYTE BALANCE; METABOLISM; OXYGEN CONSUMPTION; PHYSIOLOGY; REPRODUCIBILITY; TURTLE;

ANIMALS; BASAL METABOLISM; BODY COMPOSITION; BODY WATER; DEUTERIUM; ECOSYSTEM; OXYGEN CONSUMPTION; OXYGEN ISOTOPES; REPRODUCIBILITY OF RESULTS; SEAWATER; TURTLES; WATER-ELECTROLYTE BALANCE;

CHELONIA MYDAS; CHELONIIDAE; REPTILIA; TESTUDINES;

EID: 68249123721     PISSN: 00220949     EISSN: None     Source Type: Journal    
DOI: 10.1242/jeb.029330     Document Type: Article

References (49)

1. Anderson, N. L., Hetherington, T. E. and Williams, J. B. (2003). Validation of the doubly labeled water method under low and high humidity to estimate metabolic rate and water flux in a tropical snake (Boiga irregularis). J. Appl. Physiol. 95, 184-191.
2. Aquarone, M., Born, E. W. and Speakman, J. R. (2006). Field metabolic rates of walrus (Odobenus rosmarus) measured by the doubly-labelled water method. Aquat. Mamm. 32, 363-369.
3. Arnould, J. P. Y., Boyd, I. L. and Speakman, J. R. (1996). The relationship between foraging behaviour and energy expenditure in Antarctic Fur seals. J. Zool. (Lond.) 239, 769-782.
4. Bjorndal, K. A. (1996). Foraging ecology and nutrition of sea turtles. In The Biology of Sea Turtles (ed. P. L. Lutz and J. Musick), pp. 199-231. Boca Raton, FL: CRC Press.
5. Booth, D. T. (2002). The doubly-labeled water technique is impractical for measurement of field metabolic rate in freshwater turtles. Herpetol. Rev. 33, 105-107.
6. Butler, P. J., Green, J. A., Boyd, I. L. and Speakman, J. R. (2004). Measuring metabolic rate in the field: the pros and cons of the doubly labelled water and heart rate methods. Fund Ecol. 18, 168-183.
7. Christian, K., Green, B. and Kennett, R. (1996). Some physiological consequences of estivation by freshwater crocodiles, Crocodylus johnstoni. J. Herpetol. 30, 1-9.
8. Costa, D., Croxal, J. and Duck, C. (1989). Foraging energetics of Antarctic fur seals in relation to changes in prey availability. Ecology 70, 596-606.
9. Coulson, R. A. and Hernandez, T. (1964). Biochemistry of the Alligator: a Study of Metabolism in Slow Motion. Baton Rouge, LA: Louisiana State University Press.
10. 18O labeled H2O: comparisons between calorimeter and isotope values. In Human Energy Metabolism: Physical Activity and Energy Expenditure Measurements in Epidemiological Research Based upon Direct and Indirect Calorimetry, vol. 5 (ed. A. J. H. van Es), pp. 126-268. The Hague: CIP-gegevens Koninklijke Bibliotheek.
11. Davenport, J. (1998). Sustaining endothermy on a diet of cold jelly: energetics of the leatherback turtle Dermochelys coriacea. Br. Herpetol. Soc. Bull. 62, 4-8.
12. Drent, J., van Marken Lichtenbelt, W. D. and Wikelski, M. (1999). Effects of foraging mode on the energetics of the marine iguana, Amblyrhyncus cristalus. Funct. Ecol. 13, 493-499.
13. 2 measuring systems. J. Appl. Physiol. 51, 772-776.
14. Fossette, S., Corbel, H., Gaspar, P., LeMaho, Y. and Georges, J. (2008). An alternative technique for the long-term satellite tracking of leatherback turtles. Endanger. Species Res. 4, 33-41.
15. Grigg, G. C. (1978). Metabolic rate, Q10 and respiratory quotient (RQ) in Crocodylus porosus, and some generalizations about low RQ in reptiles. Physiol. Zool. 51, 354-360.
16. Jones, T. T., Reina, R. D., Darveau, C.-A. and Lutz, P. L. (2007). Ontogeny of energetics in leatherback (Dermochelys coriacea) and olive ridley (Lepidochelys olivacea) sea turtle hatchlings. Comp. Biochem. Physiol. 147A, 313-322.
17. Khalil, F. (1947). Excretion in reptiles 1, Non-protein nitrogen constituents of the urine of the sea turtle, Chelonia mydas (L.). J. Biol. Chem. 171, 611-616.
18. Kleiber, M. (1961 ). The Fire of Life: An Introduction to Animal Energetics. New York: John Wiley.
19. Kraemer, J. E. and Bennett, S. H. (1981). Utilization of posthatching yolk in loggerhead sea turtles, Caretta caretta. Copeia 1981, 406-411.
20. Krol, E. and Speakman, J. R. (1999). Isotope dilution spaces of mice injected simultaneously with deuterium, tritium and oxygen-18. J. Exp. Biol. 202, 2839-2849.
21. Lifson, N. and McClintock, R. (1966). Theory of use of the turnover rates of body water for measuring energy and material balance. J. Theor. Biol. 12, 46-74.
22. Lutcavage, M. and Lutz, P. L. (1986). Metabolic rate and food energy requirements of the leatherback sea turtle, Dermochelys coriaceia. Copeia 1986, 796-798.
23. Lydersen, C., Griffiths, D., Gjertz, I. and Wiig, O. (1992). A tritiated water experiment on a male Atlantic walrus (Odobemus rosmarus rosmarus). Mar. Mamm. Sci. 8, 418-420.
24. Nagy, K. A. (1980). CO2 production in animals: analysis of potential errors in the doubly labeled water method. Am. J. Physiol. 238, R466-R473.
25. Nagy, K. A. (1983a). The Doubly Labeled Water (3HH180) Method: A Guide to Its Use. Los Angeles, CA: University of California Publ. No. 12-1417.
26. Nagy, K. A. (1983b). Ecological energetics. In Lizard Ecology: Studies of a Model Organism (ed. R. B. Huey, E. R. Pianka and T. W. Schoener), pp. 24-54. Cambridge, MA: Harvard University Press.
27. Nagy, K. A. (1989). Doubly-labeled water studies of vertebrate physiological ecology. In Stable Isotopes in Ecolgical Research (ed. P. W. Rundel, J. R. Ehleringer and K. A. Nagy), pp. 270-287. New York: Springer-Verlag.
28. Nagy, K. A. and Knight, M. H. (1989). Comparative field energetics of a kalahari skink (Mabuya striata) and gecko (Pachydactylus bibroni). Copeia 1989, 13-17.
29. Nagy, K. A. and Shoemaker, V. H. (1984). Field energetics and food consumption of the Galapagos marine iguana, Amblyrhyncus cristatus. Physiol. Zool. 57, 281-290.
30. Nagy, K. A., Girard, I. A. and Brown, T. K. (1999). Energetics of free-ranging mammals, reptiles, and birds. Annu. Rev. Nutr. 19, 247-277.
31. Ortiz, R. M., Patterson, R. M., Wade, C. E. and Byers, F. M. (2000). Effects of acute fresh water exposure on water flux rates and osmotic responses in Kemp's ridley sea turtles (Lepidochelys kempi). Comp. Biochem. Physiol. 127A, 81-87.
32. Reilly, J. J. and Fedak, M. A. (1991). Rates of water turnover and energy expenditure of free-living male common seals (Phoca vitulina). J. Zool. (Lond.) 223, 431-468.
33. Reina, R. D. (2000). Salt gland blood flow in the hatchling green turtle, Chelonia mydas. J. Comp. Physiol. B 170, 573-580.
34. Reina, R. D., Jones, T. T. and Spotila, J. R. (2002). Salt and water regulation by the leatherback sea turtle Dermochelys coriacea. J. Exp. Biol. 205, 1853-1860.
35. Roe, J. H., Georges, A. and Green, B. (2008). Energy and water flux during terrestrial estivation and overland movement in a freshwater turtle. Physiol. Biochem. Zool. 81, 570-583.
36. Schmidt-Nielsen, K. and Skadhauge, E. (1967). Function of the excretory system of the crocodile (Crocodylus acutus). Am. J. Physiol. 212, 973-980.
37. Schoeller, D. A. (1988). Measurement of energy expenditure in free-living humans by using doubly labeled water. J. Nutr. 118, 1278-1289.
38. Schoeller, D. A., Ravussin, E., Schutz, Y., Acheson, K. J., Baertschi, P. and Jéquier, E. (1986). Energy expenditure by doubly labeled water: validation in humans and proposed calculation. Am. J. Physiol. 250, R823-R830.
39. Southwood, A. L., Andrews, R. D., Paladino, F. V. and Jones, D. R. (2005). Effects of swimming and diving behavior on body temperatures of Pacific leatherbacks in tropical seas. Physiol. Biochem. Zool. 78, 285-297.
40. Southwood, A. L., Reina, R. D., Jones, V. S., Speakman, J. R. and Jones, D. R. (2006). Seasonal metabolism of juvenile green turtles (Chelonia mydas) at Heron Island, Australia. Can. J. Zool. 84, 125-135.
41. Sparling, C. E., Thompson, D., Fedak, M. A., Gallon, S. M. and Speakman, J. R. (2008). Estimating the field metabolic rate of pinnipeds: doubly-labelled water gets the seal of approval. Fund Ecol. 22, 245-254.
42. Speakman, J. R. (1993). How should we calculate CO2 production in DLW studies of animals? Fund Ecol. 7, 746-750.
43. Speakman, J. R. (1997). Doubly Labelled Water: Theory and Practice. London: Chapman and Hall.
44. Speakman, J. R. and Racey, P. A. (1987). The energetics of pregnancy and lactation in the brown long-eared bat, Plecotus auritus. In Recent Advances in the Study of Bats (ed M. B. Fenton, P. A. Racey and J. M. V. Rayner), pp. 368-393. Cambridge: Cambridge University Press.
45. 2 production from doubly labelled water in humans. Am. J. Physiol. 264, E912-E917.
46. Thorson, T. B. (1968). Body fluid partitioning in reptilia. Copeia 3, 592-601.
47. Trullas, S., Spotila, J. R. and Paladino, F. V. (2006). Energetics during hatchling dispersal of the olive ridley turtle Lepidochelys olivacea using doubly labeled water. Physiol. Biochem. Zool. 79, 389-399.
48. Wallace, B. P. and Jones, T. T. (2008). What makes marine turtles go: a review of metabolic rates and their consequences. J. Exp. Mar. Biol. Ecol. 356, 8-24.
49. Wallace, B. P., Williams, C. L., Paladino, F. V., Morreale, S. J., Lindstrom, R. T. and Spotila, J. R. (2005). Bioenergetics and diving activity of interesting leatherback turtles Dermochelys coriacea at Parque Nacional Marino Las Baulas, Costa Rica. J. Exp. Biol. 208, 3873-3884.