GFP-tagged proteins visualized by freeze-fracture immuno-electron microscopy: A new tool in cellular and molecular medicine

Journal of Cellular and Molecular Medicine

Volumn 13, Issue 7, 2009, Pages 1381-1390

  Robenek, Horst ^a   Buers, Insa ^a   Hofnagel, Oliver ^a   Lorkowski, Stefan ^a   Severs, Nicholas J ^b  

^a UNIVERSITY OF MÜNSTER   (Germany)

^b NATIONAL HEART AND LUNG INSTITUTE   (United Kingdom)

Author keywords

Electron microscopy; Freeze fracture electron microscopy; GFP; Immunocytochemistry; Niemann Pick disease; PAT family proteins

Indexed keywords

CD4 ANTIGEN; GREEN FLUORESCENT PROTEIN; HYBRID PROTEIN; PERILIPIN; PHOSPHOPROTEIN;

ARTICLE; FLUORESCENT ANTIBODY TECHNIQUE; FREEZE FRACTURE; HUMAN; IMMUNOELECTRON MICROSCOPY; MEDICAL TECHNOLOGY; METABOLISM; METHODOLOGY; REPRODUCIBILITY; WESTERN BLOTTING;

ANTIGENS, CD4; BIOMEDICAL TECHNOLOGY; BLOTTING, WESTERN; FLUORESCENT ANTIBODY TECHNIQUE; FREEZE FRACTURING; GREEN FLUORESCENT PROTEINS; HUMANS; MICROSCOPY, IMMUNOELECTRON; PHOSPHOPROTEINS; RECOMBINANT FUSION PROTEINS; REPRODUCIBILITY OF RESULTS;

EID: 68149179047     PISSN: 15821838     EISSN: None     Source Type: Journal    
DOI: 10.1111/j.1582-4934.2008.00407.x     Document Type: Article

References (22)

1. Chalfie M, Tu Y, Euskirchen G, et al. Green fluorescent protein as a marker for gene expression. Science. 1984 263 : 802 5.
2. Prior IA, Harding A, Yan J, et al. GTP-dependent segregation of H-ras from lipid rafts is required for biological activity. Nat Cell Biol. 2001 3 : 368 75.
3. Misteli T, Spector DL. Applications of the green fluorescent protein in cell biology and biotechnology. Nat Biotech. 1997 15 : 961 64.
4. Hanson MR, Köhler, RH. GFP imaging: methodology and application to investigate cellular compartmentation in plants. J Exp Bot. 2001 52 : 529 39.
5. van Roessel P, Brand AH. Imaging into the future: visualizing gene expression and protein interactions with fluorescent proteins. Nat Cell Biol. 2002 4 : E15 20.
6. Chudakov DM, Lukyanov S, Lukyanov KA. Fluorescent proteins as a toolkit for in vivo imaging. Trends Biotech. 2005 23 : 605 13.
7. Verkusha VV, Lukyanov KA. The molecular properties and applications of Anthozoa fluorescent proteins and chromoproteins. Nat Biotech. 2004 22 : 289 96.
8. Terskikh A, Fradkov A, Ermakova G, et al. "Fluorescent timer": Protein that changes color with time. Science. 2000 290 : 1585 8.
9. Forster A, Pannell R, Drynan LF, et al. The invertor knock-in conditional chromosomal translocation mimic. Nature Meth. 2005 2 : 27 30.
10. Ward BM, Moss B. Visualization of intracellular movement of vaccinia virus virions containing a green fluorescent protein-B5R membrane protein chimera. J Virol. 2001 75 : 4802 13.
11. Thomsen P, Roepstorff K, Stahlhut M, van Deurs B. Caveolae are highly immobile plasma membrane microdomains, which are not involved in constitutive endocytic trafficking. Mol Biol Cell. 2002 13 : 238 50.
12. Martin S, Driessen K, Nixon SJ, et al. Regulated localization of Rab18 to lipid droplets. J Biol Chem. 2005 280 : 42325 35.
13. Follet-Gueye ML, Pagny S, Faye L, et al. An improved chemical fixation method suitable for immunogold localization of green fluorescent protein in the golgi apparatus of tobacco bright yellow (BY-2) cells. J Histochem Cytochem. 2003 51 : 931 40.
14. Luby-Phelps K, Ning G, Fogerty J, Besharse JC. Visualization of identified GFP-expressing cells by light and electron microscopy. J Histochem Cytochem. 2003 51 : 271 4.
15. Paupard, MC, Miller A, Grant B, et al. Immuno-EM localization of GFP-tagged yolk proteins in C. elegans using microwave fixation. J Histochem Cytochem. 2001 49 : 949 56.
16. Fujimoto K. Freeze-fracture replica electron microscopy combined with SDS digestion for cytochemical labeling of integral membrane proteins. Application to the immunogold labeling of intercellular junctional complexes. J Cell Sci. 1995 108 : 3443 9.
17. Robenek H, Hofnagel O, Buers I, et al. Butyrophilin controls milk fat globule secretion. Proc Natl Acad Sci USA. 2006 103 : 10385 90.
18. Robenek H, Hofnagel O, Buers I, et al. Adipophilin-enriched domains in the ER membrane are sites of lipid droplet biogenesis. J Cell Sci. 2006 119 : 4215 24.
19. Robenek H, Lorkowski S, Schnoor M, Troyer D. Spatial integration of TIP47 and adipophilin in macrophage lipid bodies. J Biol Chem. 2005 280 : 5789 94.
20. Robenek H, Robenek MJ, Buers I, et al. Lipid droplets gain PAT family proteins by interaction with specialized plasma membrane domains. J Biol Chem. 2005 280 : 26330 8.
21. Severs NJ. Freeze-fracture electron microscopy. Nat Protocols. 2007 2 : 547 76.
22. Iwashima Y, Eto M, Hata A, et al. Advanced glycation end products-induced gene expression of scavenger receptors in cultured human monocyte-derived macrophages. Biochem Biophys Res Commun. 200 277 : 529 39.