Comparative toxicity of preservatives on immortalized corneal and conjunctival epithelial cells

Journal of Ocular Pharmacology and Therapeutics

Volumn 25, Issue 2, 2009, Pages 113-119

  Epstein, Seth P ^a   Ahdoot, Michael ^a   Marcus, Edward ^a   Asbell, Penny A ^a  

^a MOUNT SINAI SCHOOL OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

2 PROPANOL; BENZALKONIUM CHLORIDE; CHLORBUTOL; EDETIC ACID; FORMALDEHYDE; METHYL PARABEN; PERBORATE SODIUM; PRESERVATIVE; THIOMERSAL; UNCLASSIFIED DRUG; 4 HYDROXYBENZOIC ACID ESTER; BENZALKONIUM; BORIC ACID; EYE DROPS;

ARTICLE; CELL VIABILITY; COMPARATIVE STUDY; CONJUNCTIVA EPITHELIUM; CONTROLLED STUDY; CORNEA CELL; CORNEA EPITHELIUM; CYTOTOXICITY; HUMAN; HUMAN CELL; HUMAN CELL CULTURE; OPTICAL DENSITY; TISSUE CULTURE; CELL LINE; CELL SURVIVAL; CONJUNCTIVA; CYTOLOGY; DRUG EFFECT; DRUG SCREENING; EPITHELIUM CELL;

BENZALKONIUM COMPOUNDS; BORATES; CELL LINE; CELL SURVIVAL; CHLOROBUTANOL; CONJUNCTIVA; DRUG EVALUATION, PRECLINICAL; EPITHELIAL CELLS; EPITHELIUM, CORNEAL; HUMANS; OPHTHALMIC SOLUTIONS; PARABENS; PRESERVATIVES, PHARMACEUTICAL; THIMEROSAL;

EID: 68149132922     PISSN: 10807683     EISSN: None     Source Type: Journal    
DOI: 10.1089/jop.2008.0098     Document Type: Article

References (28)

1. Noecker, R. Effects of common ophthalmic preservatives on ocular health. Adv. Ther.18:205-215, 2001. (Pubitemid 33131188)
2. Spickett, C. Studies of Cellular Responses to Purite and Other Preservatives. Strathclyde, UK: University of Strathclyde, 2001.
3. Asbell, P.A. Tear Substitutes: Past, Present and Future. Fifth 5th International Symposium on Ocular Pharmacology and Therapeutics (ISOPT), Monte Carlo, Monaco, March 11-14, 2004.
4. Noecker, R., Herrygers, L., and Anwaruddin, R. Corneal and conjunctival changes caused by commonly used glaucoma medications. Cornea. 23:490-496, 2004. (Pubitemid 38857008)
5. Noecker, R. Protecting the ocular surface in glaucoma and keratoconjunctivitis sicca. A Supplement to Refractive Eyecare® for Ophthalmologists. 8:1-10, 2004.
6. Soni, M.G., Taylor, S.L., Greenberg, N.A., et al. Evaluation of the health aspects of methyl paraben: a review of the publish literature. Food Chem. Toxicol. 40:1335-1373, 2002.
7. Debbasch, C., Brignole, F., Pisella, P.J., et al. Quaternary ammoniums and other preservatives' contribution in oxidative stress and apoptosis on Chang conjunctival cells. Invest. Ophthalmol. Vis. Sci. 42:642-652, 2001. (Pubitemid 32206903)
8. Hazardous Substances Data Bank. Thimerosal. September 10, 2004. http://toxnet.nlm.nih.gov/cgi-bin/sis/search/f?./temp/∼ YLMMa2:1. Retrieved January 9, 2005.
9. Van Horn, D.L., Edelhauser, H.F., Prodanovich, G., et al. Effect of the ophthalmic preservative thimerosal on rabbit and human corneal endothelium. Invest. Ophthalmol. Vis. Sci. 16:273-280, 1977. (Pubitemid 8086396)
10. Lazarus, H.M., Imperia, P.S., Botti, R.E., et al. An in vitro method which assesses corneal epithelial toxicity due to antineoplastic, preservative and antimicrobial agents. Lens Eye Toxic. Res. 6:59-85, 1989.
11. Tripathi, B.J., Tripathi, R.C., and Kolli, S.P. Cytotoxicity of ophthalmic preservatives on human corneal epithelium. Lens Eye Toxic. Res. 9:361-375, 1992.
12. Chetoni, P., Burgalassi, S., Monti, D., et al. Ocular toxicity of some corneal penetration enhancers evaluated by electrophysiology measurements on isolated rabbit corneas. Toxicol. In Vitro. 17:497-504, 2003. (Pubitemid 36776791)
13. Burgalassi, S., Chetoni, P., Monti, D., et al. Cytotoxicity of potential ocular permeation enhancers evaluated on rabbit and human corneal epithelial cell lines. Toxicol. Lett. 122:1-8, 2001. (Pubitemid 32524053)
14. Takahashi, N. Quantitative cytotoxicity of preservatives evaluated in cell culture with Chang's human conjunctival cells-effect of temperature on cytotoxicity. Jpn. J. Ophthalmol. 26:234-238, 1982. (Pubitemid 12080619)
15. Takahashi, N. Cytotoxicity of mercurial preservatives in cell culture. Ophthalmic Res. 14:63-69, 1982. (Pubitemid 12183798)
16. Offord, E.A., Sharif, N.A., Mace, K., et al. Immortalized human corneal epithelial cells for ocular toxicity and inflammation studies. Invest. Ophthalmol. Vis. Sci. 40:1091-1101, 1999. (Pubitemid 29211446)
17. Walker, T.L., and Kahn, C.R. Human Corneal Epithelial Cells with Extended Lifespan. U.S. Patent 5,672,498 dated September 30, 1997.
18. Dixon, W.J.Z., and Massey, F.J. Introduction to Statistical Analysis. 3rd ed. New York: McGraw-Hill, 1969:223.
19. Mossman, T. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J. Immunol. Methods. 65:55-63, December 16, 1983.
20. Hoel, P.H., Port, S.C., and Stone, C.J. Introduction to Statistical Theory, Vol.2, Chapter 3. Boston: Houghton Mifflin, 1971:52-110.
21. Asbell, P.A., and Potapova, N. Effects of topical antiglaucoma medications on the ocular surface. Ocul. Surf. 3:27-40, 2005. (Pubitemid 40347578)
22. De Saint Jean, M., Brignole, F., Bringuier, A.F., et al. Effects of benzalkonium chloride on growth and survival of Chang conjunctival cells. Invest. Ophthalmol. Vis. Sci. 40:619-630, 1999. (Pubitemid 29109440)
23. Marple, B., Roland, P., and Benninger, M. Safety review of benzalkonium chloride used as a preservative in intranasal solutions: an overview of confl icting data and opinions. Otolaryngol. Head Neck Surg. 130:131-141, 2004. (Pubitemid 38113927)
24. Graf, P., Hallen, H., and Juto, J.E. Benzalkonium chloride in a decongestant nasal spray aggravates rhinitis medicamentosa in healthy volunteers. Clin. Exp. Allergy. 25:395-400, 1995.
25. Hallen, H., and Graf, P. Benzalkonium chloride in nasal decongestive sprays has a long-lasting adverse effect on the nasal mucosa of healthy volunteers. Clin. Exp. Allergy. 25:401-405, 1995.
26. Labbé, A., Pauly, A., Liang, H., et al. Comparison of toxicological profiles of benzalkonium chloride and polyquaternium-1: an experimental study. J. Ocul. Pharmacol. Ther. 22:267-278, 2006. (Pubitemid 44689667)
27. Huhtala, A., Alajuuma, P., Burgalassi, S., et al. A collaborative evaluation of the cytotoxicity of two surfactants by using the human corneal epithelial cell line and the WST-1 test. J. Ocul. Pharmacol. Ther. 19:11-21, 2003. doi:10.1089/108076803762718079. (Pubitemid 36249876)
28. Brasnu, E., Brignole-Baudouin, F., Riancho, L., Warnet, J.M., Baudouin, C. Comparative study on the cytotoxic effects of benzalkonium chloride on the Wong-Kilbourne derivative of Chang conjunctival and IOBA-NHC cell lines. Mol. Vis. 14:394-402, 2008.