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2O). One set was then treated with a 10 molar excess ethanolic solution of DEPC for 30 min at 27 °C, while the other set acting as the control was treated just with ethanol. The reaction was terminated using excess Tris-HC1 buffer (pH 8.3). The optical density of the DEPC-containing tubes was measured at around 240 nm using respective control tubes containing ethanol on a Shimatzu 1601 spectrophotometer.
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2O). One set was then treated with a 10 molar excess ethanolic solution of DEPC for 30 min at 27 °C, while the other set acting as the control was treated just with ethanol. The reaction was terminated using excess Tris-HC1 buffer (pH 8.3). The optical density of the DEPC-containing tubes was measured at around 240 nm using respective control tubes containing ethanol on a Shimatzu 1601 spectrophotometer.
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68149157767
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2] (pH 7.4) were treated with various concentrations of ethanolic solutions of DEPC (0-50 mM) for 30 min at 27 °C. The reaction was terminated using excess Tris-HCl buffer (pH 8.3). Spectra were measured between 400 and 800 nm against water containing equal of ethanol on a Jasco V630 spectrophotometer.
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2] (pH 7.4) were treated with various concentrations of ethanolic solutions of DEPC (0-50 mM) for 30 min at 27 °C. The reaction was terminated using excess Tris-HCl buffer (pH 8.3). Spectra were measured between 400 and 800 nm against water containing equal volumes of ethanol on a Jasco V630 spectrophotometer.
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68149161594
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MS experiments were carried out on Applied Biosystem's API 4000Q trap system coupled with an Agilent 1200 series liquid Chromatograph provided with Analyst 1.4.2 software. A mobile phase was methanol and water with 0.1% formic acid. The electrospray ionization was used in positive polarity. The source parameters are ion spray voltage 5.5k, nebulizer gas 20 psi, heater gas 55 psi, and source temperature 350 °C. A C18 column (55 × 2 × 3 μm) was used with a flow rate of 0.3 mL/min.
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MS experiments were carried out on Applied Biosystem's API 4000Q trap system coupled with an Agilent 1200 series liquid Chromatograph provided with Analyst 1.4.2 software. A mobile phase was methanol and water with 0.1% formic acid. The electrospray ionization was used in positive polarity. The source parameters are ion spray voltage 5.5k, nebulizer gas 20 psi, heater gas 55 psi, and source temperature 350 °C. A C18 column (55 × 2 × 3 μm) was used with a flow rate of 0.3 mL/min.
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