Fusobacterium nucleatum enters normal human oral fibroblasts in vitro

Journal of Periodontology

Volumn 80, Issue 7, 2009, Pages 1174-1183

  Dabija Wolter, D J ^a   Cimpan, Mihaela Roxana ^a   Costea, Daniela E ^a   Johannessen, Anne Christine ^a   Sørnes, Steinar ^a   Neppelberg, Evelyn ^a   Al Haroni, Mohammed ^a   Skaug, Nils ^b   Bakken, Vidar ^a,c  

^a UNIVERSITY OF BERGEN   (Norway)

^b UNIVERSITY OF BERGEN   (Norway)

^c HAUKELAND UNIVERSITY HOSPITAL   (Norway)

Author keywords

Fibroblasts; Host parasite interactions; Oral; Pathology

Indexed keywords

ARTICLE; BACTERIUM ADHERENCE; CELL CULTURE; COCULTURE; CYTOLOGY; FIBROBLAST; FUSOBACTERIACEAE INFECTION; FUSOBACTERIUM NUCLEATUM; GINGIVA; HOST PATHOGEN INTERACTION; HUMAN; MICROBIOLOGY; PATHOGENICITY; PHYSIOLOGY; REFERENCE VALUE; SPECIES DIFFERENCE; VIRULENCE;

BACTERIAL ADHESION; CELLS, CULTURED; COCULTURE TECHNIQUES; FIBROBLASTS; FUSOBACTERIACEAE INFECTIONS; FUSOBACTERIUM NUCLEATUM; GINGIVA; HOST-PATHOGEN INTERACTIONS; HUMANS; REFERENCE VALUES; SPECIES SPECIFICITY; VIRULENCE;

EID: 68049135496     PISSN: 00223492     EISSN: None     Source Type: Journal    
DOI: 10.1902/jop.2009.090051     Document Type: Article

References (47)

1. Bolstad AI, Jensen HB, Bakken V. Taxonomy, biology, and periodontal aspects of Fusobacterium nucleatum. Clin Microbiol Rev 1996;9:55-71.
2. Gmu R, Munson MA, Wade WG. Genotypic and phenotypic characterization of fusobacteria from Chinese and European patients with inflammatory periodontal diseases. Syst Appl Microbiol 2006;29: 120-130.
3. Gharbia SE, Shah HN, Lawson PA, Haapasalo M. Distribution and frequency of Fusobacterium nucleatum subspecies in the human oral cavity. Oral Microbiol Immunol 1990;5:324-327.
4. Moore WE, Moore LV. The bacteria of periodontal diseases. Periodontol 2000 1994;5:66-77.
5. Feng Z, Weinberg A. Role of bacteria in health and disease of periodontal tissues. Periodontol 2000 2006; 40:50-76.
6. Kinder Haake S, Hutchins Meyer D, Fives-Taylor PM, Schenkein H. Periodontal diseases. In: Lamont RJ, Burne RA, Lantz MS, LeBlanc DJ, eds. Oral Microbiology and Immunology. Washington, DC: ASM Press; 2006:253-279.
7. Johnson JD, Chen R, Lenton PA, Zhang G, Hinrichs JE, Rudney JD. Persistence of extracrevicular bacterial reservoirs after treatment of aggressive periodon-titis. J Periodontol 2008;79:2305-2312.
8. Rudney JD, Chen R. The vital status of human buccal epithelial cells and the bacteria associated with them. Arch Oral Biol 2006;51:291-298.
9. Rudney JD, Chen R, Sedgewick GJ. Intracellular Ac-tinobacillus actinomycetemcomitans and Porphyromo-nas gingivalis in buccal epithelial cells collected from human subjects. Infect Immun 2001;69:2700-2707.
10. Rudney JD, Chen R, Sedgewick GJ. Actinobacillus actinomycetemcomitans, Porphyromonas gingivalis, and Tannerella forsythensis are components of a polymicrobial intracellular flora within human buccal cells. J Dent Res 2005;84:59-63.
11. Rudney JD, Chen R, Zhang G. Streptococci dominate the diverse flora within buccal cells. J Dent Res 2005; 84:1165-1171.
12. Colombo AV, Silva CM, Haffajee A, Colombo AP. Identification of oral bacteria associated with crevicu-lar epithelial cells from chronic periodontitis lesions. J Med Microbiol 2006;55:609-615.
13. Han YW, Shi W, Huang GT, et al. Interactions between periodontal bacteria and human oral epithelial cells: Fusobacterium nucleatum adheres to and invades epithelial cells. Infect Immun 2000;68:3140-3146.
14. Hasegawa Y, Mans JJ, Mao S, et al. Gingival epithelial cell transcriptional responses to commensal and opportunistic oral microbial species. Infect Immun 2007; 75:2540-2547.
15. Ji S, Shin JE, Kim YS, Oh JE, Min BM, Choi Y. Toll-like receptor 2 and NALP2 mediate induction of human beta-defensins by Fusobacterium nucleatum in gingival epithelial cells. Infect Immun 2009;77:1044-1052.
16. Han YW, Redline RW, Li M, Yin L, Hill GB, McCormick TS. Fusobacterium nucleatum induces premature and term stillbirths in pregnant mice: Implication of oral bacteria in preterm birth. Infect Immun 2004;72:2272-2279.
17. Saito A, Inagaki S, Kimizuka R, et al. Fusobacterium nucleatum enhances invasion of human gingival epithelial and aortic endothelial cells by Porphyromonas gingivalis. FEMS Immunol Med Microbiol 2008;54: 349-355.
18. Edwards AM, Grossman TJ, Rudney JD. Fusobacte-rium nucleatum transports noninvasive Streptococcus cristatus into human epithelial cells. Infect Immun 2006;74:654-662.
19. Diaz PI, Zilm PS, Rogers AH. Fusobacterium nucleatum supports the growth of Porphyromonas gingivalis in oxygenated and carbon-dioxide-depleted environments. Microbiology 2002;148:467-472.
20. Eick S, Pfister W. Efficacy of antibiotics against periodontopathogenic bacteria within epithelial cells: An in vitro study. J Periodontol 2004;75:1327-1334. (Pubitemid 39517370)
21. Gursoy UK, Kononen E, Uitto VJ. Intracellular replication of fusobacteria requires new actin filament formation of epithelial cells. APMIS 2008;116:1063-1070.
22. Madianos PN, Papapanou PN, Nannmark U, Dahlén G, Sandros J. Porphyromonas gingivalis FDC381 multiplies and persists within human oral epithelial cells in vitro. Infect Immun 1996;64:660-664.
23. Metzger Z, Blasbalg J, Dotan M, Weiss EI. Enhanced attachment of Porphyromonas gingivalis to human fibroblasts mediated by Fusobacterium nucleatum. J Endod 2009;35:82-85.
24. Ozaki M, Miyake Y, Shirakawa M, Takemoto T, Okamoto H, Suginaka H. Binding specificity of Fuso-bacterium nucleatum to human erythrocytes, poly-morphonuclear leukocytes, fibroblasts, and HeLa cells. J Periodontal Res 1990;25:129-134.
25. Kuru L, Parkar MH, Griffiths GS, Newman HN, Olsen I. Flow cytometry analysis of gingival and periodontal ligament cells. J Dent Res 1998;77:555-564.
26. Giannopoulou C, Cimasoni G. Functional characteristics of gingival and periodontal ligament fibroblasts. J Dent Res 1996;75:895-902.
27. Somerman MJ, Archer SY, Imm GR, Foster RA. A comparative study of human periodontal ligament cells and gingival fibroblasts in vitro. J Dent Res 1988; 67:66-70.
28. Hatakeyama J, Tamai R, Sugiyama A, Akashi S, Sugawara S, Takada H. Contrasting responses of human gingival and periodontal ligament fibroblasts to bacterial cell-surface components through the CD14/Toll-like receptor system. Oral Microbiol Immunol 2003;18:14-23.
29. Pathirana RD, O'Brien-Simpson NM, Visvanathan K, Hamilton JA, Reynolds EC. Flow cytometric analysis of adherence of Porphyromonas gingivalis to oral epithelial cells. Infect Immun 2007;75:2484-2492.
30. Agerer F, Waeckerle S, Hauck CR. Microscopic quantification of bacterial invasion by a novel antibody-independent staining method. J Microbiol Methods 2004;59:23-32.
31. Costea DE, Dimba EO, Loro LL, Johannessen AC, Vintermyr OK. Proliferation and differentiation in orga-notypic serum free cultures of normal human oral mucosa. In: Varma AK, Kowalski LP, Herter NT, eds. 8th International Congress on Oral Cancer, vol. 8. Rio de Janeiro: Rajkamal Electric Press; 2002:108-113.
32. Harper LJ, Piper K, Common J, Fortune F, Mackenzie IC. Stem cell patterns in cell lines derived from head and neck squamous cell carcinoma. J Oral Pathol Med 2007;36:594-603.
33. Jensen PH, Cressey LI, Gjertsen BT, et al. Cleaved intracellular plasminogen activator inhibitor 2 in human myeloleukaemia cells is a marker of apoptosis. Br J Cancer 1994;70:834-840.
34. Pils S, Schmitter T, Neske F, Hauck CR. Quantification of bacterial invasion into adherent cells by flow cyto-metry. J Microbiol Methods 2006;65:301-310.
35. Mosiman VL, Patterson BK, Canterero L, Goolsby CL. Reducing cellular autofluorescence in flow cytometry: An in situ method. Cytometry 1997;30:151-156.
36. Pi SH, Lee SK, Hwang YS, Choi MG, Kim EC. Differential expression of periodontal ligament-specific markers and osteogenic differentiation in human pap- illoma virus 16-immortalized human gingival fibro-blasts and periodontal ligament cells. J Periodontal Res 2007;42:104-113.
37. Locke M, Hyland PL, Irwin CR, Mackenzie IC. Modulation of gingival epithelial phenotypes by interactions with regionally defined populations of fibroblasts. J Periodontal Res 2008;43:279-289.
38. Liu H, Redline RW, Han YW. Fusobacterium nucleatum induces fetal death in mice via stimulation of TLR4-mediated placental inflammatory response. J Immunol 2007;179:2501-2508.
39. Poltorak A, Ricciardi-Castagnoli P, Citterio S, Beutler B. Physical contact between lipopolysaccharide and toll-like receptor 4 revealed by genetic complementation. Proc Natl Acad Sci USA 2000;97:2163-2167.
40. Takeda K, Akira S. Microbial recognition by Toll-like receptors. J Dermatol Sci 2004;34:73-82.
41. Kikkert R, Laine ML, Aarden LA, van Winkelhoff AJ. Activation of toll-like receptors 2 and 4 by gram-negative periodontal bacteria. Oral Microbiol Immunol 2007;22:145-151.
42. Wilson M, McNab R, Henderson B. Bacterial invasion as a virulence mechanism. In: Wilson M, McNab R, Henderson B, eds. Bacterial Disease Mechanisms: An Introduction to Cellular Microbiology. Cambridge, U.K.: Cambridge University Press; 2002:405-465.
43. Brook I. Recovery of anaerobic bacteria from clinical specimens in 12 years at two military hospitals. J Clin Microbiol 1988;26:1181-1188.
44. Civen R, Jousimies-Somer H, Marina M, Borenstein L, Shah H, Finegold SM. A retrospective review of cases of anaerobic empyema and update of bacteriology. Clin Infect Dis 1995;20(Suppl. 2):S224-S229.
45. Kajiya T, Uemura T, Kajiya M, et al. Pyogenic liver abscess related to dental disease in an immunocom-petent host. Intern Med 2008;47:675-678.
46. Kozarov EV, Dorn BR, Shelburne CE, Dunn WA Jr., Progulske-Fox A. Human atherosclerotic plaque contains viable invasive Actinobacillus actinomycetem-comitans and Porphyromonas gingivalis. Arterioscler Thromb Vasc Biol 2005;25:e17-e18.
47. Hill GB. Preterm birth: Associations with genital and possibly oral microflora. Ann Periodontol 1998;3:222-232.