Immunoassays are not created equal

Journal of Thrombosis and Haemostasis

Volumn 7, Issue 8, 2009, Pages 1256-1259

  Warkentin, Ted E ^a,b   Linkins, L A ^a  

^a MCMASTER UNIVERSITY   (Canada)

^b HAMILTON REGIONAL LABORATORY MEDICINE PROGRAM   (Canada)

Author keywords

[No Author keywords available]

Indexed keywords

IMMUNOGLOBULIN; PLATELET ACTIVATING ANTIBODY; THROMBOCYTE ANTIBODY; THROMBOCYTE FACTOR 4; UNCLASSIFIED DRUG;

AGGLUTINATION TEST; DIAGNOSTIC VALUE; ENZYME LINKED IMMUNOSORBENT ASSAY; HEPARIN INDUCED THROMBOCYTOPENIA; HUMAN; IMMUNOASSAY; INTERMETHOD COMPARISON; NOTE; PARTICLE GEL IMMUNOASSAY; PRIORITY JOURNAL; PROBABILITY; QUANTITATIVE ANALYSIS; SENSITIVITY AND SPECIFICITY; SEROTONIN RELEASE ASSAY; THROMBOCYTE ACTIVATION; THROMBOCYTE COUNT;

HEMOSTASIS; HEPARIN; HUMANS; IMMUNOASSAY; THROMBOCYTOPENIA;

EID: 67949112765     PISSN: 15387933     EISSN: 15387836     Source Type: Journal    
DOI: 10.1111/j.1538-7836.2009.03489.x     Document Type: Note

References (23)

1. Warkentin TE, Chong BH, Greinacher A. Heparin-induced thrombocytopenia: towards consensus. Thromb Haemost 1998; 79: 1-7.
2. Greinacher A, Pötzsch B, Amiral J, Dummel V, Eichner A, Mueller-Eckhardt C. Heparin-associated thrombocytopenia: Isolation of the antibody and characterization of a multimolecular PF4-heparin complex as the major antigen. Thromb Haemost 1994; 71: 247-51.
3. Bakchoul T, Giptner A, Bein G, Santoso S, Sachs UJH. Prospective evaluation of immunoassays for the diagnosis of heparin-induced thrombocytopenia. J Thromb Haemost. 2009; 7: 1260-5.
4. Lo GK, Sigouin CS, Warkentin TE. What is the potential for overdiagnosis of heparin-induced thrombocytopenia? Am J Hematol 2007; 82: 1037-43.
5. Warkentin TE, Sheppard JI. Testing for heparin-induced thrombocytopenia antibodies. Transfus Med Rev 2006; 20: 259-72.
6. Amiral J, Bridey F, Dreyfus M, Vissoc AM, Fressinaud E, Wolf M, Meyer D. Platelet factor 4 complexes to heparin is the target for antibodies generated in heparin-induced thrombocytopenia. Thromb Haemost 1992; 68: 95-6.
7. Visentin GP, Moghaddam M, Beery SE, McFarland JG, Aster RH. Heparin is not required for detection of antibodies associated with heparin-induced thrombocytopenia/thrombosis. J Lab Clin Med 2001; 138: 22-31.
8. Amiral J, Vissac AM. Role of heparin-dependent antigens in immune heparin-induced thrombocytopenia. In: Warkentin TE, Greinacher A, eds. Heparin-Induced Thrombocytopenia, 4th edn. New York: Informa Healthcare USA, Inc., 2007: 131-47.
9. Kelton JG, Sheridan D, Santos A, Smith J, Steeves K, Smith C, Brown C, Murphy WG. Heparin-induced thrombocytopenia: Laboratory studies. Blood 1988; 72: 925-30.
10. Lindhoff-Last E, Gerdsen F, Ackermann H, Bauersachs R. Determination of heparin-platelet factor 4-IgG antibodies improves diagnosis of heparin-induced thrombocytopenia. Br J Haematol 2001; 113: 886-90.
11. Meyer O, Aslan T, Koster A, Kiesewetter H, Salama A. Report of a patient with heparin-induced thrombocytopenia type II associated with IgA antibodies only. Clin Appl Thromb Hemost 2006; 12: 373-5.
12. Meyer O, Salama A, Pittet N, Schwind P. Rapid detection of heparin-induced platelet antibodies with particle gel immunoassay (ID-HPF4). Lancet 1999; 354: 1525-6.
13. Pouplard C, Gueret P, Fouassier M, Ternisien C, Trossaert M, Régina S, Gruel Y. Prospective evaluation of the '4Ts' score and particle gel immunoassay specific to hearin/PF4 for the diagnosis of heparin-induced thrombocytopenia. J Thromb Haemost 2007; 5: 1373-9.
14. Bryant A, Low J, Austin S, Joseph JE. Timely diagnosis and management of heparin-induced thrombocytopenia in a frequent request, low incidence single centre using clinical 4T's score and particle gel immunoassay. Br J Haematol 2008; 143: 721-6.
15. Warkentin TE, Sheppard JI, Raschke R, Greinacher A. Performance characteristics of a rapid assay for anti-PF4/heparin antibodies, the particle immunofiltration assay. J Thromb Haemost 2007; 5: 2308-10.
16. Eichler P, Raschke R, Lubenow N, Meyer O, Schwind P, Greinacher A. The new ID-heparin/PF4 antibody test for rapid detection of heparin-induced antibodies in comparison with functional and antigenic assays. Br J Haematol 2002; 116: 887-91.
17. Lo GK, Juhl D, Warkentin TE, Sigouin CS, Eichler P, Greinacher A. Evaluation of pretest clinical score (4 T's) for the diagnosis of heparin-induced thrombocytopenia in two clinical settings. J Thromb Haemost 2006; 4: 759-65.
18. Greinacher A, Michels I, Kiefel V, Mueller-Eckhard C. A rapid and sensitive test for diagnosing heparin-induced thrombocytopenia. Thromb Haemost 1991; 66: 734-6.
19. Greinacher A, Juhl D, Strobel U, Wessel A, Lubenow N, Selleng K, Eichler P, Warkentin TE. Heparin-induced thrombocytopenia: A prospective study on the incidence, platelet-activating capacity and clinical significance of anti-PF4/heparin antibodies of the IgG, IgM, and IgA classes. J Thromb Haemost 2007; 5: 1666-73.
20. Warkentin TE, Sheppard JI, Moore JC, Sigouin CS, Kelton JG. Quantitative interpretation of optical density measurements using PF4-dependent enzyme-immunoassays. J Thromb Haemost 2008; 6: 1304-12.
21. Warkentin TE, Hayward CPM, Smith CA, Kelly PM, Kelton JG. Determinants of donor platelet variability when testing for heparin-induced thrombocytopenia. J Lab Clin Med 1992; 120: 371-9.
22. Sheridan D, Carter C, Kelton JG. A diagnostic test for heparin-induced thrombocytopenia. Blood 1986; 67: 27-30.
23. Eichler P, Budde U, Haas S, Kroll H, Loreth RM, Meyer O, Pachmann U, Pötzsch B, Schabel A, Albrecht D, Greinacher A. First workshop for detection of heparin-induced antibodies: Validation of the heparin-induced platelet-activation test (HIPA) in comparison with a PF4/ heparin ELISA. Thromb Haemost 1999; 81: 625-9.