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Volumn 131, Issue 13, 2009, Pages 4953-4961

Morpholino monolayers: Preparation and label-free DNA analysis by surface hybridization

Author keywords

[No Author keywords available]

Indexed keywords

ACID MOLECULES; ANALYTE; APPLIED POTENTIALS; CAPACITIVE RESPONSE; DNA ANALYSIS; DNA PROBE; ELECTROCHEMICAL METHODS; GOLD SUPPORT; HYBRIDIZATION REACTIONS; INTERFACIAL CAPACITANCE; INTERFACIAL CHARGE; LABEL FREE; LABEL-FREE MONITORING; LIFE SCIENCE RESEARCH; LIMITS OF QUANTIFICATION; LINEAR RELATIONSHIPS; MILLIMETER RANGE; MOBILE IONS; MOLECULAR MECHANISM; POISSON-BOLTZMANN; QUANTITATIVE COMPARISON; SECONDARY STRUCTURES; SINGLE-STRANDED DNA; SURFACE CAPACITANCE; SURFACE ELECTRIC FIELDS; TARGET COVERAGE; TARGET RECOGNITION; TARGET SPECIES;

EID: 67949097240     PISSN: 00027863     EISSN: None     Source Type: Journal    
DOI: 10.1021/ja810051q     Document Type: Article
Times cited : (55)

References (79)
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    • Backbone charges on DNA strands can also contribute to ionic screening; however, this contribution is expected to be relatively minor due to constraints imposed by their connectivity and the higher mass (lower mobility) of the polymer backbone
    • Backbone charges on DNA strands can also contribute to ionic screening; however, this contribution is expected to be relatively minor due to constraints imposed by their connectivity and the higher mass (lower mobility) of the polymer backbone.
  • 56
    • 25744449978 scopus 로고    scopus 로고
    • tMCP was estimated from mass density of MCP, d ) 1.07 g cm-3, molar mass of MCP, m ) 92 g mol-1, and surface coverage of alkanethiol monolayers on gold, σ 4.7 × 1014 molecules cm-2 (Strong, L.; Whitesides, G. M. Langmuir 1988, 4, 546-558). tMCP ) σm/(dNA).
    • tMCP was estimated from mass density of MCP, d ) 1.07 g cm-3, molar mass of MCP, m ) 92 g mol-1, and surface coverage of alkanethiol monolayers on gold, σ ) 4.7 × 1014 molecules cm-2 (Strong, L.; Whitesides, G. M. Langmuir 1988, 4, 546-558). tMCP ) σm/(dNA).
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    • Gene Tools, personal communication
    • Moulton, J., Gene Tools, personal communication.
    • Moulton, J.1
  • 58
    • 84869558826 scopus 로고    scopus 로고
    • tP (4.9 × 1012 chains cm-2)(1 × 10-14 cm2 nm-2)(20 nt chain-1) (0.53 nm3 nt-1) ) 0.52 nm.
    • tP ) (4.9 × 1012 chains cm-2)(1 × 10-14 cm2 nm-2)(20 nt chain-1) (0.53 nm3 nt-1) ) 0.52 nm.
  • 59
    • 67949097750 scopus 로고    scopus 로고
    • A possible explanation for suppression of multivalent phosphate anions, as suggested by the model, is that the dielectric strength inside a probe layer is too low to stabilize multiply ionized phosphate species
    • A possible explanation for suppression of multivalent phosphate anions, as suggested by the model, is that the dielectric strength inside a probe layer is too low to stabilize multiply ionized phosphate species.
  • 60
    • 84869590052 scopus 로고    scopus 로고
    • In the model, electrostatic and partitioning penalty energies (eq 5) combine such that a displacement in VDC can be compensated through adjustment of the βj coefficients. This degree of freedom was fixed by assuming βNa, 0 for the morpholino monolayer
    • Na+) 0 for the morpholino monolayer.
  • 61
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    • Although a three-layer model consisting of an MCP layer, unhybridized probes, and a solution-side layer of more solvated morpholino-DNA hybrids might be more realistic, we continue to use two layers, as this was sufficient to reproduce experiment. Addition of a third, wellsolvated and thus high-capacitance layer would, in any case, exert a minor effect, given that smaller capacitances dominate when arranged in series
    • Although a three-layer model consisting of an MCP layer, unhybridized probes, and a solution-side layer of more solvated morpholino-DNA hybrids might be more realistic, we continue to use two layers, as this was sufficient to reproduce experiment. Addition of a third, wellsolvated and thus high-capacitance layer would, in any case, exert a minor effect, given that smaller capacitances dominate when arranged in series.
  • 63
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    • VDC is assumed to not affect the dielectric constant.
    • VDC is assumed to not affect the dielectric constant.
  • 74


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.