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Backbone charges on DNA strands can also contribute to ionic screening; however, this contribution is expected to be relatively minor due to constraints imposed by their connectivity and the higher mass (lower mobility) of the polymer backbone
-
Backbone charges on DNA strands can also contribute to ionic screening; however, this contribution is expected to be relatively minor due to constraints imposed by their connectivity and the higher mass (lower mobility) of the polymer backbone.
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tMCP was estimated from mass density of MCP, d ) 1.07 g cm-3, molar mass of MCP, m ) 92 g mol-1, and surface coverage of alkanethiol monolayers on gold, σ 4.7 × 1014 molecules cm-2 (Strong, L.; Whitesides, G. M. Langmuir 1988, 4, 546-558). tMCP ) σm/(dNA).
-
tMCP was estimated from mass density of MCP, d ) 1.07 g cm-3, molar mass of MCP, m ) 92 g mol-1, and surface coverage of alkanethiol monolayers on gold, σ ) 4.7 × 1014 molecules cm-2 (Strong, L.; Whitesides, G. M. Langmuir 1988, 4, 546-558). tMCP ) σm/(dNA).
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tP (4.9 × 1012 chains cm-2)(1 × 10-14 cm2 nm-2)(20 nt chain-1) (0.53 nm3 nt-1) ) 0.52 nm.
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tP ) (4.9 × 1012 chains cm-2)(1 × 10-14 cm2 nm-2)(20 nt chain-1) (0.53 nm3 nt-1) ) 0.52 nm.
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67949097750
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A possible explanation for suppression of multivalent phosphate anions, as suggested by the model, is that the dielectric strength inside a probe layer is too low to stabilize multiply ionized phosphate species
-
A possible explanation for suppression of multivalent phosphate anions, as suggested by the model, is that the dielectric strength inside a probe layer is too low to stabilize multiply ionized phosphate species.
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In the model, electrostatic and partitioning penalty energies (eq 5) combine such that a displacement in VDC can be compensated through adjustment of the βj coefficients. This degree of freedom was fixed by assuming βNa, 0 for the morpholino monolayer
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Na+) 0 for the morpholino monolayer.
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Although a three-layer model consisting of an MCP layer, unhybridized probes, and a solution-side layer of more solvated morpholino-DNA hybrids might be more realistic, we continue to use two layers, as this was sufficient to reproduce experiment. Addition of a third, wellsolvated and thus high-capacitance layer would, in any case, exert a minor effect, given that smaller capacitances dominate when arranged in series
-
Although a three-layer model consisting of an MCP layer, unhybridized probes, and a solution-side layer of more solvated morpholino-DNA hybrids might be more realistic, we continue to use two layers, as this was sufficient to reproduce experiment. Addition of a third, wellsolvated and thus high-capacitance layer would, in any case, exert a minor effect, given that smaller capacitances dominate when arranged in series.
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VDC is assumed to not affect the dielectric constant.
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VDC is assumed to not affect the dielectric constant.
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