Freezing equine semen: The effect of combinations of semen extenders and glycerol on post-thaw motility

Australian Veterinary Journal

Volumn 87, Issue 7, 2009, Pages 275-279

  Scherzer, J ^a   Fayrer Hosken, R A ^a   Aceves, M ^a   Hurley, D J ^a   Ray, L E ^b   Jones, L ^b   Heusner, G L ^b  

^a UNIVERSITY OF GEORGIA   (United States)

^b UNIVERSITY OF GEORGIA   (United States)

Author keywords

Cryopreservation; Extender; Glycerol; Horses; Semen

Indexed keywords

CRYOPROTECTIVE AGENT; GLYCEROL;

ANIMAL; ANIMAL DISEASE; ARTICLE; COMPARATIVE STUDY; CRYOPRESERVATION; HORSE; MALE; METHODOLOGY; PHYSIOLOGY; RANDOMIZATION; SPERM; SPERM PRESERVATION; SPERMATOZOON MOTILITY;

ANIMALS; CRYOPRESERVATION; CRYOPROTECTIVE AGENTS; GLYCEROL; HORSES; MALE; RANDOM ALLOCATION; SEMEN; SEMEN PRESERVATION; SPERM MOTILITY;

EQUIDAE;

EID: 67949094332     PISSN: 00050423     EISSN: None     Source Type: Journal    
DOI: 10.1111/j.1751-0813.2009.00452.x     Document Type: Article

References (22)

1. Vidament M, Dupere AM, Julienne P et al. Equine frozen semen: Freezability and fertility field results. Theriogenology 1997 48 : 907 917.
2. Kirk ES, Squires EL, Graham JK. Comparison of in vitro laboratory analyses with the fertility of cryopreserved stallion spermatozoa. Theriogenology 2005 64 : 1422 439.
3. Alvarenga MA, Papa FO, Landim-Alvarenga FC, Medeiros ASL. Amides as cryoprotectants for freezing stallion semen: A review. Anim Reprod Sci 2005 89 : 105 113.
4. Samper JC, Morris CA. Current methods for stallion semen cryopreservation: A survey. Theriogenology 1998 49 : 895 903.
5. Barker CAV. Pregnancy in a mare resulting from frozen epididymal spermatozoa. Can J Comp Med 1957 21 : 47 50.
6. Smith AU, Polge C. Survival of spermatozoa at low temperatures. Nature 1950 166 : 668 669.
7. Squires EL, Keith SL, Graham JK. Evaluation of alternative cryoprotectants for preserving stallion spermatozoa. Theriogenology 2004 62 : 1056 1065.
8. Devireddy RV, Swanlund DJ, Olin T et al. Cryopreservation of equine sperm: optimal cooling rates in the presence and absence of cryoprotective agents determined using differential scanning calorimetry. Biol Reprod 2002 66 : 222 231.
9. Mazur P. Freezing of living cells: mechanisms and implications. Am J Physiol Cell Physiol 1984 247 : C125 142.
10. Fuller BJ. Cryoprotectants: the essential antifreezes to protect life in the frozen state. Cryo Lett 2004 25 : 375 388.
11. Jasko DJ, Hathaway JA, Schaltenbrand VL, Simper WD, Squires EL. Effect of seminal plasma and egg yolk on motion characteristics of cooled stallion spermatozoa. Theriogenology 1992 37 : 1241 1252.
12. Alghamdi AS, Troedsson MHT, Xue JL, Crabo BG. Effect of seminal plasma concentration and various extenders on postthaw motility and glass wool-Sephadex filtration of cryopreserved stallion semen. Am J Vet Res 2002 63 : 880 885.
13. Batellier F, Vidament M, Fauquant J et al. Advances in cooled semen technology. Anim Reprod Sci 2001 68 : 181 190.
14. Braun J, Hochi S, Oguri N, Sato K, Torres-Boggino F. Effect of different protein supplements on motility and plasma membrane integrity of frozen-thawed stallion spermatozoa. Cryobiology 1995 32 : 487 492.
15. Cochran JD, Amann RP, Froman DP, Pickett BW. Effects of centrifugation, glycerol level, cooling to 5°C, freezing rate and thawing rate on the post-thaw motility of equine sperm. Theriogenology 1984 22 : 25 38.
16. Mantovani R, Rora A, Falomo ME, Bailoni L, Vincenti L. Comparison between glycerol and ethylene glycol for the cryopreservation of equine spermatozoa: semen quality assessment with standard analyses and with the hypoosmotic swelling test. Reprod Nutr Dev 2002 42 : 217 226.
17. Aurich JE, Kuhne A, Hoppe H, Aurich C. Seminal plasma affects membrane integrity and motility of equine spermatozoa after cryopreservation. Theriogenology 1996 46 : 791 797.
18. Brinsko SP, Varner DD, Love CC et al. Effect of feeding a DHA-enriched nutriceutical on the quality of fresh, cooled and frozen stallion semen. Theriogenology 2005 63 : 1519 1527.
19. Fayrer-Hosken R, Abreu-Barbosa C, Heusner G, Jones L. Cryopreservation of Stallion Spermatozoa with INRA96 and Glycerol. Journal of Equine Veterinary Science 2009 28 : 672 676.
20. Wessel MT, Ball BA. Step-wise dilution for removal of glycerol from fresh and cryopreserved equine spermatozoa. Anim Reprod Sci 2004 84 : 147 156.
21. Pillet E, Batellier F, Duchamp G, et al. High fertility rates with stallion sperm cryopreserved in INRA96 based extender are not predicted by in vitro parameters. Animal Reproduction Science 2009 107 : 339 340.
22. Pillet E, Batellier F, Duchamp G, et al. Freezing stallion semen in INRA96-based extender improves fertility rates in comparison with INRA82. Dairy Science and Technology 2008 88 : 257 265.