Identification and Characterisation of a Copper-inducible Metallothionein Gene from Cockle, Anadara granosa

Sains Malaysiana

Volumn 38, Issue 3, 2009, Pages 287-294

  Wong, Kok Kee ^a   Mat Daud, Noor Arniwati ^a   Othman, Roohaida ^a   Wan, Kiew Lian ^a   Zubir, Din ^b   Surif, Salmuah ^a  

^a UNIVERSITI KEBANGSAAN MALAYSIA   (Malaysia)

^b SCHOOL OF PHYSICS   (Malaysia)

Author keywords

Cockle; Copper; Metallothionein; Subtractive hybridisation

Indexed keywords

EID: 67749110275     PISSN: 01266039     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (37)

1. Altschul, S.F., Madden, T.L., Schärfer, A.A., Zhang, J., Zhang, Z., Miller, W. & Lipman, D.J. 1997. Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucl. Acids Res. 25: 3389-3402. (Pubitemid 27359211)
2. Barsyte, D., White, K.N. & Lovejoy, DA. 1999. Cloning and characterization of metallothionein cDNAs in the mussel Mytilus edulis L. digestive gland. Comp. Biol. Physiol. 122C: 287-296. (Pubitemid 29070795)
3. Bebianno. M.J. &Machado,L.M. 1997. Concentrations of metals and metallothioneins in Mytilus galloprovincialis along the south coast of Portugal. Mar. Pollut Bull. 34: 666-671.
4. Blasco, J. & Puppo, J. 1999. Effect of heavy metals (Cu, Cd & Pb) on aspartate and alanine aminotransferase in Ruditapes philippinarum (Mollusca: Bivalvia). Comp. Biol. Physiol. 122C: 253-263. (Pubitemid 29070791)
5. Cajaraville, M.P., Bebianno, M.J., Blasco, J., Porte, C., Sarasquete, C. & Viarengo, A. 2000. The use of biomarkers to assess the impact of pollution in coastal environments of the Iberian Peninsula: a practical approach. Sci. Total Environ. 247: 295-311. (Pubitemid 30145681)
6. Chan, M.K., Othman, R., Zubir, D. & Salmijah, S. 2002. Induction of a putative metallothionein gene in the blood cockle, Anadara granosa, exposed to cadmium. Comp. Biol. Physiol. 131C: 123-132. (Pubitemid 34185374)
7. Chomczynski, P. & Sacchi, N. 1987. Single step method of RNA isolation by acid guanidinium thiocyanate-phenolchloroform. Anal. Biochem. 162: 156-159. (Pubitemid 17064313)
8. Clayton, M.E., Steinmann, R. & Fent, K. 2000. Different expression patterns of heat shock proteins hsp 60 and hsp 70 in zebra mussels (Dreissenapolymorpha) exposed to copper and tributyltin. Aqua. Toxicol. 47: 213-226.
9. Correia, A.D., Livingstone, D.R. & Costa, M.H. 2002. Effects of water-borne copper on metallothonein and lipid peroxidation in the marine amphipod Gammarus locusta. Mar. Environ. Res. 54: 357-360. (Pubitemid 35286449)
10. Czupryn, M. & Palchuk, K. 1990. Determination of metals in metallothionein preparation by atomic absorption spectroscopy. Methods Enzymol. 205: 415-418.
11. de Astudillo, L.R., Yen, I.C., Agard, J., Bekele, I. & Hubbard, R. 2002. Heavy metals in green mussel (Perna viridis) and oysters (Crassostrea sp.) from Trinidad and Venezuela. Arch. Environ. Contam. Toxicol. 42: 410-415. (Pubitemid 34454403)
12. Department of Environment (DOE), Ministry of Science, Technology & the Environment Malaysia, 2002. Laporan Alam Sekitar.
13. Eduardo, M., Elena, B.K., Axaceli, D.A., Dulce, M.LA., Olimpia, C., Nilda, G. & Antonio, A. 2005. Cloning, tissue expression and metal inducibility of an ubiquitous metallothionein from Panulirus argus. Gene. 361: 140-148. (Pubitemid 41506942)
14. Frohman, M.A., Dush, M.K. & Martin, G.R. 1988. Rapid production of full-length cDNAs from rare transcripts: amplification using a single gene-specific oligonucleotide primer. Proc. Natl. Acad. Sci. USA. 85: 8998-9002. (Pubitemid 19001832)
15. George, S.G. 1992. Biochemical and cytological assessments of metal toxicity in marine animals. In Heavy metals in the marine environment. New York, Plenum Press, edited by W.R, Furness, P.S. Rainbow, pp. 123-142.
16. Glickman, L.T. 1991. Animals as sentinels of environmental health hazards. Washington: National Academy Press.
17. Jing, G., Li, Y., Xie, L.P. & Zhang, R.Q. 2006. Metal accumulation and enzyme activities in gills and digestive gland of pearl oyster (Pinctada fucata) exposed to copper. Comp. Biol. Physiol. 144C: 184-190. (Pubitemid 44602581)
18. Kägi.J.H.R.&Schäffer.A. 1988.Biochemistry of metallothionein. Biochem. 27: 8515-8909.
19. Kägi, J.H.R. & Valee, B.L. 1960. Metallothionein. A cadmium and zinc containing protein from equine renal cortex. J. Biol. Chem. 235: 3460-3465.
20. Klaassen,C.D.&Liu,J.1998. Inductionofmetallothioneinasanadaptive mechanismaffectingthemagnitudeandprogressionoftoxicologicalinjury.Environ. HealthPerspect.106:297-300.(Pubitemid28196054)
21. Liang, L.N., He, B., Jiang, G.B., Chen, D.Y. & Yao, Z.W. 2004. Evaluation of molluscs as biomonitors to investigate heavy metal contaminations along Chinese Bohai, Sea. Sci. Total Environ. 324: 105-113. (Pubitemid 38481869)
22. Linde, A.R., Sanchez-Galan, S., Klein, D., Garcia-Vasquez, E. & Summer, K.H. 1999. Metallothionein and heavy metals in brown trout (Salmo trutta) and European eel (Anguilla anguilla): A comparative study. Ecotoxicol. Environ. Safety 44: 168-173. (Pubitemid 29496355)
23. Malaysian Food Act & Regulations. 1996. Laws of Malaysia. 7th Ed. pp. 219. Kuala Lumpur: MDC Publishers Printers Sdn Bhd.
24. McCarthy, J.F. & Shugart, L. R. 1990. Biomarkers of environmental contamination. Florida, Lewis Publishers, Boca Raton, pp. 457.
25. Mat, I., Maah, M. J. & Johari, A. 1994. Trace metal geochemical associations in sediments from culture-bed of Anadara granosa. Mar. Pollut. Bull. 28: 319-323.
26. Nasci, C., Da Ros, L., Nesto, N., Sperni, L., Passarini, F. & Pavoni, B. 2002. Biochemical and histochemical responses to environmental contaminations in clam, Tapesphilippinarum, transplanted to different polluted areas of Venice Lagoon, Italy. Mar. Environ. Res. 50: 425-430. (Pubitemid 30811332)
27. Nemer, M., Wilkinson, D.G., Travaglini, E.C., Sternberg, E J. & Butt, T.R. 1985. Sea urchin metallothionein sequence: key to an evolutionary diversity. Proc. Natl. Acad. Sci. U.S.A. 82:4992-4994.
28. 2+: Analysis by metal summation, SH determination and spectrofluorometry. Comp. Biochem. Physiol. 126C: 113-122.
29. Rand, G.M. & Petrocelli, S. R. 1985. Introduction to fundamentals of aquatic toxicology. Hemisphere Co., Washington, pp. 1-30.
30. Ringwood, A.H. & Brouwer, M. 1993. Expression of constitutive and metal-inducible metallothioneins in oyster embryos (Crassostrea virginica). Comp. Biochem. Physiol. 106B: 523-529.
31. Thompson,J.D.,Higgins,D.G.&Gibson,T.J.1994.CLUSTALW: Improvingthesensitivityofprogressive multiplesequencealignmentthroughsequenceweighting,position- specificgappenaltiesandweightmatrixchoice.Nucl.AcidsRes.22:4673-4680. (Pubitemid24354800)
32. Thomson, J. D., Pirie, B.J.S. & George, S.G. 1985. Cellular metal distribution in Pacific oyster, Crassostrea gigas (Thun.) determined by quantitative" X-ray microphone analysis. J. Exp. Mar. Biol. Ecol. 85: 47-45.
33. 4 in carp. J. Environ. Sci. Health. 31: 627-635.
34. Unger, M.E., Chen, T.T., Murphy, C.M., Vestling, M.M., Fenselau, C. & Roesjadi, G. 1991. Primary structure of molluscan metallothioneins deduced from PCR-amplified cDNA and mass spectrometry of purified proteins. Biochim. Biophys.Acta. 1074: 371-377.
35. Vasak. M. & Hasler, D.W. 2000. Metallothioneins: new functional and structural insights. Curr. Opin. Chem. Biol. 4: 177-183. (Pubitemid 30189309)
36. WHO. 1982. Toxicological evaluation of certain food additives and contaminants. Joint FAOAVHO Expert Committee on Food Additives, WHO Food Additives Series no 17, World Health Organization, Geneva, pp. 28-35.
37. Zorita, I., Ortiz-Zarragoitia, M., Soto, M. & Cajaraville. M.P. 2006. Biomarkers in mussels from a copper site gradient (Visnes, Norway): An integrated biochemical, histochemical and histological study. Aqua. Toxicol. 78S: S109-S116.