Blockade of epithelial sodium channels improves sperm motility in asthenospermia patients

International Journal of Andrology

Volumn 32, Issue 4, 2009, Pages 330-336

  Kong, Xiang Bin ^a   Ma, Hua Gang ^b   Li, Hong Gang ^a   Xiong, Cheng Liang ^a  

^a HUAZHONG UNIVERSITY OF SCIENCE AND TECHNOLOGY   (China)

^b YANTAI YUHUANGDING HOSPITAL   (China)

Author keywords

Asthenospermia; EIPA; Epithelial Na+ channel; Motility; Sperm

Indexed keywords

AMILORIDE DERIVATIVE; EPITHELIAL SODIUM CHANNEL; EPITHELIAL SODIUM CHANNEL ALPHA; EPITHELIAL SODIUM CHANNEL DELTA; UNCLASSIFIED DRUG;

ANIMAL TISSUE; ARTICLE; ASTHENOSPERMIA; COMPUTER ANALYSIS; CONTROLLED STUDY; HUMAN; HUMAN TISSUE; IMMUNOBLOTTING; IMMUNOHISTOCHEMISTRY; INHIBITION KINETICS; LEYDIG CELL; MALE; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN LOCALIZATION; RAT; SERTOLI CELL; SPERMATID; SPERMATOCYTE; SPERMATOGONIUM; SPERMATOZOON MIDPIECE; SPERMATOZOON MOTILITY; SPERMATOZOON TAIL; TESTIS;

AMILORIDE; ANIMALS; ASTHENOZOOSPERMIA; BLOTTING, WESTERN; CASE-CONTROL STUDIES; EPITHELIAL SODIUM CHANNEL; FERTILITY AGENTS, MALE; HUMANS; IMMUNOHISTOCHEMISTRY; MALE; RATS; RATS, SPRAGUE-DAWLEY; SODIUM CHANNEL BLOCKERS; SPERM MOTILITY; SPERMATOZOA; TESTIS;

EID: 67651244657     PISSN: 01056263     EISSN: 13652605     Source Type: Journal    
DOI: 10.1111/j.1365-2605.2008.00864.x     Document Type: Article

References (19)

1. Alvarez de la Rosa, D., Canessa, C. M., Fyfe, G. K. Zhang, P. (2000) Structure and regulation of amiloride-sensitive sodium channels. Annual Review of Physiology 62, 573 594.
2. Ambrosini, A., Zolese, G., Ambrosi, S., Bertoli, E., Mantero, F., Boscaro, M. Balercia, G. (2005) Idiopathic infertility: effect of palmitoylethanolamide (a homologue of anandamide) on hyperactivated sperm cell motility and Ca21 influx. Journal of Andrology 26, 429 436.
3. Canessa, C. M., Schild, L., Buell, G., Thorens, B., Gautschi, I., Horisberger, J. D. Rossier, B. C. (1994) Amiloride-sensitive epithelial Na+ channel is made of three homologous subunits. Nature 367, 463 467.
4. Chen, M. J. Bongso, A. (1999) Comparative evaluation of two density gradient preparations for sperm separation for medically assisted conception. Human Reproduction 14, 759 764.
5. Demarco, I. A., Espinosa, F., Edwards, J., Sosnik, J., De La Vega-Beltran, J. L., Hockensmith, J. W., Kopf, G. S., Darszon, A. Visconti, P. E. (2003) Involvement of a Na+\HCO-3 cotransporter in mouse sperm capacitation. Journal of Biological Chemistry 278, 7001 7009.
6. Djelidi, S., Fay, M., Cluzeaud, F., Escoubet, B., Eugene, E., Capurro, C., Bonvalet, J. P., Farman, N. Blot-Chabaud, M. (1997) Transcriptional regulation of sodium transport by vasopressin in renal cells. Journal of Biological Chemistry 272, 32919 32924.
7. Hernandez-Gonzalez, E. O., Sosnik, J., Edwards, J., Acevedo, J. J., Mendoza-Lujambio, I., Lopez-Gonzalez, I., Demarco, I., Wertheimer, E., Darszon, A. Visconti, P. E. (2006) Sodium and epithelial sodium channels participate in the regulation of the capacitation-associated hyperpolarization in mouse sperm. Journal of Biological Chemistry 281, 5623 5633.
8. Kalab, P., Visconti, P., Leclerc, P. Kopf, G. S. (1994) p95, the major phosphotyrosine-containing protein in mouse spermatozoa, is a hexokinase with unique properties. Journal of Biological Chemistry 269, 3810 3817.
9. Kellenberger, S. Schild, L. (2002) Epithelial sodium channel\degenerin family of ion channels: a variety of functions for a shared structure. Physiological Reviews 82, 735 767.
10. Laemmli, U. K. (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227, 680 685.
11. Li, H. G., Ding, X. F., Liao, A. H., Kong, X. B. Xiong, C. L. (2007) Expression of CatSper family transcripts in the mouse testis during post-natal development and human ejaculated spermatozoa: relationship to sperm motility. Molecular Human Reproduction 13, 299 306.
12. Morisawa, M. (1994) Cell signaling mechanisms for sperm motility. Zoological Science 11, 647 662.
13. Saha, C., Eckert, G. J., Ambrosius, W. T., Chun, T. Y., Wagner, M. A., Zhao, Q. Pratt, J. H. (2005) Improvement in blood pressure with inhibition of the epithelial sodium channel in blacks with hypertension. Hypertension 46, 481 487.
14. Shigaev, A., Asher, C., Latter, H., Garty, H. Reuveny, E. (2000) Regulation of sgk by aldosterone and its effects on the epithelial Na(+) channel. American Journal of Physiology Renal Physiology 278, F613 F619.
15. Sigman, M., Glass, S., Campagnone, J. Pryor, J. L. (2006) Carnitine for the treatment of idiopathic asthenospermia: a randomized, double-blind, placebo-controlled trial. Fertility and Sterility 85, 1409 1414.
16. Teiwes, J. Toto, R. D. (2007) Epithelial sodium channel inhibition in cardiovascular disease. A potential role for amiloride. American Journal of Hypertension 20, 109 117.
17. Woollhead, A. M. Baines, D. L. (2006) Forskolin-induced cell shrinkage and apical translocation of functional enhanced green fluorescent protein-human alphaENaC in H441 lung epithelial cell monolayers. Journal of Biological Chemistry 281, 5158 5168.
18. Yamamura, H., Ugawa, S., Ueda, T., Nagao, M. Shimada, S. (2004) Capsazepine is a novel activator of the delta subunit of the human epithelial Na+ channel. Journal of Biological Chemistry 279, 44483 44489.
19. Zhou, Y., Huang, H., Chang, H. H., Du, J., Wu, J. F., Wang, C. Y. Wang, M. H. (2006) Induction of renal 20-hydroxyeicosatetraenoic acid by clofibrate attenuates high-fat diet-induced hypertension in rats. Journal of Pharmacology and Experimental Therapeutics 317, 11 18.