Effect of different concentrations of ascorbic acid on motility, membrane integrity and chromatin status of frozen-thawed canine spermatozoa within six hours of storage at 37°C

Reproduction in Domestic Animals

Volumn 44, Issue SUPPL. 2, 2009, Pages 354-358

  Eulenberger, K ^a,b   Schafer Somi S ^a   Aurich, C ^a  

^a UNIVERSITY OF VETERINARY MEDICINE   (Austria)

^b Eu Centre for Artificial Insemination and Embryo Transfer   (Austria)

Author keywords

[No Author keywords available]

Indexed keywords

ASCORBIC ACID; CRYOPROTECTIVE AGENT;

ANIMAL; ANIMAL DISEASE; CELL MEMBRANE; CHROMATIN; CONFERENCE PAPER; CRYOPRESERVATION; DOG; DRUG EFFECT; LABORATORY DIAGNOSIS; MALE; PHYSIOLOGY; SPERMATOZOON; SPERMATOZOON MOTILITY;

ANIMALS; ASCORBIC ACID; CELL MEMBRANE; CHROMATIN; CRYOPRESERVATION; CRYOPROTECTIVE AGENTS; DOGS; MALE; SPECIMEN HANDLING; SPERM MOTILITY; SPERMATOZOA;

EID: 67651202719     PISSN: 09366768     EISSN: 14390531     Source Type: Journal    
DOI: 10.1111/j.1439-0531.2009.01417.x     Document Type: Conference Paper

References (17)

1. Aurich JE, Schönherr U, Hoppe H, Aurich C, 1997: Effects of antioxidants on motility and membrane integrity of chilled-stored stallion semen. Theriogenology 48, 185 192.
2. Ceylan A, Serin I, 2007: Influence of ascorbic acid addition to the extender on dog sperm motility, viability and acrosomal integrity during cooled storage. Rev Méd Vét 158, 7, 384 387.
3. Dalvit GC, Cetica PD, Beconi MT, 1997: Effect of α-tocopherol and ascorbic acid on bovine in vitro fertilization. Theriogenology 49, 619 627.
4. Evenson D, Jost L, 2000: Sperm chromatin structure assay is useful for fertility assessment. Methods Cell Sci 22, 169 189.
5. Hatamoto LK, Bapista Sobrinho CA, Nichi M, Barnabe VH, Barnabe RC, Cortada CNM, 2006: Effects of dexamethasone treatment (to mimic stress) and vitamin E oral supplementation on the spermiogram and on seminal plasma spontaneous lipid peroxidation and antioxidant enzyme activities in dogs. Theriogenology 66, 1610 1614.
6. Linde-Forsberg C, 2001: International veterinary information services. Ithaca, NY. Document No. A1209.0501. (15sid), 2001. B. Uppsala Equex - 2 System. Available at: http://www.ivis.org.
7. Lopes S, Jurisicova A, Sun RF, 1998: Reactive oxygen species; potential cause for DNA fragmentation in human spermatozoa. Hum Reprod 13, 896 900.
8. Menezo Y, 2007: Antioxidant to reduce sperm DNA fragmentation: an unexpected adverse effect. Reprod Biomed Online 14, 418 421.
9. Michael A, Alexopoulos C, Pontiki E, Hadjipavlou-Litina D, Saratsis P, Boscos C, 2007: Effect of antioxidant supplementation on semen quality and reactive oxygen species of frozen-thawed canine spermatozoa. Theriogenology 68, 204 212.
10. Michael AJ, Alexopoulos C, Pontiki EA, Hadjipavlou-Litina DJ, Saratsis P, Ververidis HN, Boscos CM, 2008: Effect of antioxidant supplementation in semen extenders on semen quality and reactive oxygen species of chilled canine spermatozoa. Anim Reprod Sci 112, 119 135.
11. Olson PN, 1984: Disorders of the canine prostate gland. In: Proceedings of the Annual Meeting of the Society for Theriogenology, Denver, September 26-28. American College of Therlogenologists (Eds), Nashville, pp. 46 59.
12. Petrunkina AM, Gropper B, Günzel-Apel AR, Töpfer-Petersen E, 2004: Functional significance of the cell volume for detecting sperm membrane changes and predicting freezability in dog semen. Reproduction 128, 829 842.
13. Rota A, Rota A, Martini M, Milani C, Romagnoli S, 2005: Evaluation of dog semen after slow (biological freezer) or rapid (nitrogen vapours) freezing. Reprod Nutr 45, 29 37.
14. Schäfer-Somi S, Aurich C, 2007: Use of a new computer-assisted sperm-analyzer for the assessment of motility and viability of dog spermatozoa and evaluation of four different semen extenders for predilution. Anim Reprod Sci 102, 3 4.
15. Schäfer-Somi S, Kluger S, Knapp E, Klein D, Aurich C, 2006: Effects of semen extender and semen processing on motility and membrane integrity of frozen-thawed dog spermatozoa. Theriogenology 66, 173 182.
16. Seager SWJ, Fletcher WS, 1972: Collection, storage and insemination of canine semen. Lab Anim Sci 22, 177 182.
17. Yousef MI, Abdallah GA, Kamel KI, 2003: Effect of ascorbic acid and vitamin E supplementation on semen quality and biochemical parameters of male rabbits. Anim Reprod Sci 76, 99 111.